Detailed information of TA system TA030645

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): phd-doc/Doc-GNAT
• Location: 428913..430006
• Replicon: chromosome
• Accession: NZ_AP014525
• Organism: Vibrio cholerae MS6

Toxin (Protein)

• Gene name: doc
• Uniprot ID: Q9KMA2
• Locus tag: MS6_RS15820
• Protein ID: WP_000351248.1
• Coordinates: 429605..430006 (+)
• Length: 134 a.a.

Antitoxin (Protein)

• Gene name: phd
• Uniprot ID: -
• Locus tag: MS6_RS15815
• Protein ID: WP_001047177.1
• Coordinates: 428913..429605 (+)
• Length: 231 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MS6_RS19755	425140..425253	+	114	WP_001889158.1	hypothetical protein	-
MS6_RS19760	425202..425429	+	228	WP_001894457.1	DUF1289 domain-containing protein	-
MS6_RS15785	425770..426102	+	333	WP_000843587.1	type II toxin-antitoxin system RelE/ParE family toxin	-
MS6_RS15790	426089..426403	+	315	WP_000071008.1	DNA-binding transcriptional regulator	-
MS6_RS15795	426540..426974	+	435	WP_000256036.1	GNAT family N-acetyltransferase	-
MS6_RS20445	427142..427297	+	156	WP_000751734.1	hypothetical protein	-
MS6_RS15805	427422..428402	-	981	WP_041051966.1	IS5-like element ISVch5 family transposase	-
MS6_RS15810	428470..428776	+	307	Protein_465	CatB-related O-acetyltransferase	-
MS6_RS15815	428913..429605	+	693	WP_001047177.1	GNAT family N-acetyltransferase	Antitoxin
MS6_RS15820	429605..430006	+	402	WP_000351248.1	type II toxin-antitoxin system death-on-curing family toxin	Toxin
MS6_RS20245	429976..430119	+	144	WP_071908341.1	DUF3265 domain-containing protein	-
MS6_RS15825	430194..430607	+	414	WP_000049417.1	VOC family protein	-
MS6_RS15830	430821..431072	+	252	WP_001250179.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-
MS6_RS15835	431062..431349	+	288	WP_000221354.1	type II toxin-antitoxin system RelE/ParE family toxin	-
MS6_RS15840	431477..431932	+	456	WP_001245327.1	GNAT family N-acetyltransferase	-
MS6_RS15845	432254..432406	+	153	WP_001884520.1	DUF645 family protein	-
MS6_RS15850	432602..433270	+	669	WP_000043871.1	hypothetical protein	-
MS6_RS15855	433422..433709	+	288	WP_000426470.1	hypothetical protein	-
MS6_RS15860	433850..434221	+	372	WP_001164080.1	nucleotide pyrophosphohydrolase	-
MS6_RS20450	434288..434713	+	426	WP_001882332.1	DUF1778 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	qnrVC4	-	297729..445581	147852
	-	inside	Integron	qnrVC4	-	302809..445205	142396
	-	inside	IScluster/Tn	-	-	423729..428402	4673

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Death on curing protein (1-132)

Antitoxin

• GNAT domain (36-153)

Sequences

Toxin

Protein

• Length: 134 a.a.
• Molecular weight: 14608.71 Da
• Isoelectric Point: 4.0128

>T30645 WP_000351248.1 NZ_AP014525:429605-430006 [Vibrio cholerae MS6]
MDIICFPFERVIEINAFILKTEPGMKGAVDIPKLQGALGRIDNAIVYEGLDDVFEIAAKYTACIAVSHALPDANKRTGLA
VALEYLSLNDFELTQENDLLADAVRDLVIGIINETDFADILYAQYAKEQNSAL

Nucleotide

• Length: 402 bp

>T30645 NZ_AP014525:429605-430006 [Vibrio cholerae MS6]
ATGGATATCATCTGTTTTCCTTTTGAACGAGTGATCGAGATTAATGCTTTCATCCTAAAAACAGAACCAGGAATGAAAGG
AGCCGTTGATATTCCCAAGCTTCAGGGGGCATTAGGTCGAATCGATAATGCCATCGTTTATGAAGGACTGGATGATGTTT
TTGAAATCGCTGCTAAATACACTGCTTGTATAGCGGTTTCACATGCGCTACCTGATGCGAACAAACGCACAGGTTTGGCT
GTGGCACTTGAATATTTATCACTTAACGATTTTGAGCTTACGCAGGAAAACGATTTACTTGCGGATGCAGTTCGAGATCT
TGTTATCGGCATTATCAACGAAACAGATTTTGCCGACATTCTCTACGCACAATACGCAAAAGAACAAAATTCAGCTCTCT
AA

Antitoxin

Protein

• Length: 231 a.a.
• Molecular weight: 26306.64 Da
• Isoelectric Point: 8.6819

>AT30645 WP_001047177.1 NZ_AP014525:428913-429605 [Vibrio cholerae MS6]
MNLEEFQESDFDLLIKWIDSDELNYLWGGPAYVFPLTYEQIHSHCSKAEVFPYLLKVKGRHAGFVELYKVTDEQYRICRV
FISNAYRGQGLSKSMLMLLIDKARLDFSATKLSLGVFEQNTVARKCYESLGFEVVMVVVIEFGGMRCQPLRRALCFLSRF
GAIIGLTFIDSRCDMNRKVEAYGVDAVERPKIKASKKLDLTGDAGRQIVKSETKLALRTHQKTFTKLADM

Nucleotide

• Length: 693 bp

>AT30645 NZ_AP014525:428913-429605 [Vibrio cholerae MS6]
ATGAACTTAGAAGAGTTTCAAGAATCGGATTTTGACCTGCTTATAAAATGGATTGACTCAGATGAGTTGAACTATTTATG
GGGTGGCCCTGCGTATGTTTTCCCGCTGACTTATGAACAGATCCATTCCCACTGTAGCAAAGCGGAAGTATTTCCCTATC
TGCTAAAAGTGAAAGGTAGACATGCCGGGTTTGTTGAGTTGTACAAAGTCACCGATGAGCAATATCGGATTTGCCGTGTC
TTTATTTCAAATGCTTATCGTGGGCAAGGGCTATCAAAATCGATGTTGATGTTATTAATCGATAAAGCCCGATTAGATTT
TTCTGCAACCAAGCTTAGCTTAGGCGTTTTTGAACAGAACACAGTTGCTAGAAAATGCTATGAGTCATTAGGTTTTGAAG
TGGTTATGGTTGTTGTGATTGAGTTTGGCGGTATGCGTTGTCAGCCCCTTAGGCGGGCGTTATGTTTTTTATCACGTTTT
GGGGCTATAATCGGCCTAACCTTTATTGATAGTAGGTGTGATATGAATCGAAAAGTTGAAGCTTACGGCGTTGATGCTGT
TGAAAGACCGAAAATTAAGGCTAGTAAAAAGCTTGATTTGACTGGTGATGCTGGTCGTCAAATTGTGAAGTCTGAAACTA
AACTTGCTTTGCGTACTCATCAGAAGACATTCACTAAATTGGCTGATATGTAA

Structures

Toxin

• AlphaFold DB: A0A0Q0PN34