Detailed information of TA system TA030643

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-YafN
• Location: 414546..415074
• Replicon: chromosome
• Accession: NZ_AP014525
• Organism: Vibrio cholerae MS6

Toxin (Protein)

• Gene name: relE
• Uniprot ID: H9L4R3
• Locus tag: MS6_RS15700
• Protein ID: WP_000221352.1
• Coordinates: 414546..414836 (-)
• Length: 97 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: H9L4T4
• Locus tag: MS6_RS15705
• Protein ID: WP_000213183.1
• Coordinates: 414826..415074 (-)
• Length: 83 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MS6_RS20415	410103..410228	+	126	WP_001944767.1	DUF645 family protein	-
MS6_RS20420	410244..410282	+	39	WP_106019119.1	hypothetical protein	-
MS6_RS15675	410495..411499	+	1005	WP_000964925.1	LD-carboxypeptidase	-
MS6_RS15680	411652..412098	+	447	WP_000128977.1	hypothetical protein	-
MS6_RS15685	412218..413147	+	930	WP_000335802.1	hypothetical protein	-
MS6_RS20220	413144..413254	+	111	WP_071917832.1	DUF3265 domain-containing protein	-
MS6_RS15690	413282..413758	+	477	WP_001047180.1	GNAT family N-acetyltransferase	-
MS6_RS15695	413895..414410	+	516	WP_001201532.1	lipocalin family protein	-
MS6_RS15700	414546..414836	-	291	WP_000221352.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
MS6_RS15705	414826..415074	-	249	WP_000213183.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
MS6_RS15720	415645..416298	+	654	WP_000036957.1	site-specific DNA-methyltransferase	-
MS6_RS15725	416570..416995	+	426	WP_000415748.1	hypothetical protein	-
MS6_RS15730	417203..417598	+	396	WP_000649527.1	DUF3465 domain-containing protein	-
MS6_RS15735	417749..418279	+	531	WP_000415563.1	hypothetical protein	-
MS6_RS15740	418417..418917	+	501	WP_000789723.1	hypothetical protein	-
MS6_RS15745	419061..419918	+	858	WP_001279526.1	DUF2806 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	qnrVC4	-	297729..445581	147852
	-	inside	Integron	qnrVC4	-	302809..445205	142396

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-86)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 97 a.a.
• Molecular weight: 11247.23 Da
• Isoelectric Point: 10.4678

>T30643 WP_000221352.1 NZ_AP014525:c414836-414546 [Vibrio cholerae MS6]
MTYKLEFKKSALKEWKKLAVPLQQQFKKKLIERLENPHVPSAKLSGAENIYKIKLRQSGYRLVYQVENDIIVVTVLAVGK
RERSEVYTKALQRLDD

Nucleotide

• Length: 291 bp

>T30643 NZ_AP014525:c414836-414546 [Vibrio cholerae MS6]
ATGACCTATAAGCTTGAGTTCAAAAAGAGTGCTTTGAAGGAATGGAAAAAGTTAGCCGTTCCACTCCAACAGCAGTTCAA
GAAGAAACTAATAGAACGCCTTGAAAATCCACATGTTCCATCAGCCAAACTGTCTGGCGCTGAGAACATTTATAAAATAA
AACTTCGGCAGTCTGGTTATCGCTTAGTCTATCAAGTTGAAAATGACATCATCGTAGTTACTGTTTTAGCTGTCGGTAAA
CGAGAACGCAGCGAAGTTTACACTAAAGCGCTGCAACGGCTAGACGACTGA

Antitoxin

Protein

• Length: 83 a.a.
• Molecular weight: 8937.28 Da
• Isoelectric Point: 4.1079

>AT30643 WP_000213183.1 NZ_AP014525:c415074-414826 [Vibrio cholerae MS6]
MTTRILADVAASITEFKANPMKVATSAFGAPVAVLNRNEPAFYCVPASTYEIMMDKLEDLELLAIAKERLSEDSVSVNID
DL

Nucleotide

• Length: 249 bp

>AT30643 NZ_AP014525:c415074-414826 [Vibrio cholerae MS6]
ATGACCACTAGAATCCTAGCTGATGTTGCAGCAAGCATTACCGAATTTAAAGCAAACCCAATGAAAGTTGCAACTAGTGC
CTTCGGTGCTCCTGTTGCTGTACTCAACCGAAATGAGCCGGCTTTCTACTGCGTCCCTGCAAGCACCTACGAGATCATGA
TGGATAAGCTTGAAGATCTTGAGCTACTGGCTATTGCCAAAGAACGCTTAAGTGAAGACAGCGTATCGGTAAATATCGAT
GACCTATAA

Structures

Toxin

• AlphaFold DB: H9L4R3

Antitoxin

• AlphaFold DB: A0A366A3W8