Detailed information of TA system TA030642

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /BrnT(toxin)
• Location: 387086..387584
• Replicon: chromosome
• Accession: NZ_AP014525
• Organism: Vibrio cholerae MS6

Toxin (Protein)

• Gene name: -
• Uniprot ID: Q9KMK7
• Locus tag: MS6_RS15490
• Protein ID: WP_000589156.1
• Coordinates: 387086..387352 (+)
• Length: 89 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: Q9KMK6
• Locus tag: MS6_RS15495
• Protein ID: WP_000643598.1
• Coordinates: 387339..387584 (+)
• Length: 82 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MS6_RS15455	382318..382854	+	537	WP_000644491.1	nucleotidyltransferase family protein	-
MS6_RS20400	382973..383134	+	162	WP_000481782.1	hypothetical protein	-
MS6_RS19575	383323..383433	+	111	WP_071908335.1	DUF3265 domain-containing protein	-
MS6_RS15460	383485..383838	+	354	WP_000384364.1	hypothetical protein	-
MS6_RS15465	384050..384292	+	243	WP_000107461.1	hypothetical protein	-
MS6_RS15470	384544..384822	+	279	WP_000280324.1	DUF3709 domain-containing protein	-
MS6_RS20405	385197..385340	+	144	Protein_391	DUF645 family protein	-
MS6_RS20410	385394..385432	+	39	WP_082798268.1	hypothetical protein	-
MS6_RS15480	385644..386111	+	468	WP_001289288.1	OsmC family protein	-
MS6_RS15485	386239..386901	+	663	WP_000960654.1	hypothetical protein	-
MS6_RS15490	387086..387352	+	267	WP_000589156.1	BrnT family toxin	Toxin
MS6_RS15495	387339..387584	+	246	WP_000643598.1	hypothetical protein	Antitoxin
MS6_RS15500	387680..388474	+	795	WP_001911581.1	hypothetical protein	-
MS6_RS15505	388632..389636	+	1005	WP_000964920.1	LD-carboxypeptidase	-
MS6_RS15510	389924..390106	+	183	WP_000923182.1	DUF645 family protein	-
MS6_RS15515	390303..390728	+	426	WP_000403005.1	GNAT family N-acetyltransferase	-
MS6_RS15520	390868..391458	+	591	WP_000021480.1	hypothetical protein	-
MS6_RS15525	391612..391971	+	360	WP_001071529.1	VOC family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	qnrVC4	-	297729..445581	147852
	-	inside	Integron	qnrVC4	-	302809..445205	142396

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (6-81)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 89 a.a.
• Molecular weight: 10092.34 Da
• Isoelectric Point: 7.3171

>T30642 WP_000589156.1 NZ_AP014525:387086-387352 [Vibrio cholerae MS6]
MIKFEFDENKSRSNLEKHGIDFHTAQGLWNDSDLIEIPANTSDEPRYLVVGLLNGKHWSGVITYRGTNIRIISVRRSRKA
EVSLYESA

Nucleotide

• Length: 267 bp

>T30642 NZ_AP014525:387086-387352 [Vibrio cholerae MS6]
ATGATTAAATTTGAATTTGATGAAAACAAAAGCAGAAGTAATCTCGAAAAGCACGGTATTGATTTTCATACAGCTCAAGG
ATTGTGGAATGATTCAGATCTTATCGAGATCCCTGCTAACACGAGTGATGAGCCCAGATATTTGGTTGTCGGCTTACTAA
ACGGTAAGCATTGGTCAGGAGTAATTACCTACCGAGGTACGAATATCAGGATCATCTCAGTTCGGCGTTCACGGAAAGCG
GAGGTAAGCCTTTATGAAAGCGCATGA

Antitoxin

Protein

• Length: 82 a.a.
• Molecular weight: 9478.77 Da
• Isoelectric Point: 6.4832

>AT30642 WP_000643598.1 NZ_AP014525:387339-387584 [Vibrio cholerae MS6]
MKAHEFDAKFESDDDDIVMDLDLSQAKRPMHKQKRVNVDFPAWMLESLDREASRIGVTRQSIIKIWLAERLESVSHHSSV
R

Nucleotide

• Length: 246 bp

>AT30642 NZ_AP014525:387339-387584 [Vibrio cholerae MS6]
ATGAAAGCGCATGAATTTGATGCCAAGTTTGAAAGTGACGACGATGATATCGTAATGGACTTGGATCTATCGCAAGCTAA
AAGACCGATGCATAAGCAAAAACGAGTCAATGTAGATTTTCCAGCCTGGATGCTTGAGTCCTTGGACAGAGAAGCGAGTC
GTATTGGTGTAACACGTCAATCAATCATTAAAATTTGGCTGGCAGAAAGGTTAGAGAGCGTGTCTCATCATTCAAGTGTG
AGATAA

Structures

Toxin

• AlphaFold DB: A0A271VMP7

Antitoxin

• AlphaFold DB: A0A271VME2