Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) /GNAT-DUF1778
Location 369717..370483 Replicon chromosome
Accession NZ_AP014525
Organism Vibrio cholerae MS6

Toxin (Protein)


Gene name - Uniprot ID Q9K2P7
Locus tag MS6_RS15355 Protein ID WP_000982260.1
Coordinates 369717..370214 (-) Length 166 a.a.

Antitoxin (Protein)


Gene name - Uniprot ID Q9K2J6
Locus tag MS6_RS15360 Protein ID WP_000246253.1
Coordinates 370211..370483 (-) Length 91 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MS6_RS19485 365040..365162 + 123 WP_080314583.1 DUF3265 domain-containing protein -
MS6_RS15310 365169..365873 + 705 WP_000087610.1 HNH endonuclease -
MS6_RS15315 366031..366369 + 339 WP_000713853.1 hypothetical protein -
MS6_RS15320 366523..366840 - 318 WP_001180243.1 type II toxin-antitoxin system RelE/ParE family toxin -
MS6_RS15325 366859..367101 - 243 WP_000557292.1 type II toxin-antitoxin system Phd/YefM family antitoxin -
MS6_RS15330 367345..367731 + 387 WP_001015867.1 NUDIX hydrolase -
MS6_RS19490 367788..367901 + 114 WP_001900214.1 hypothetical protein -
MS6_RS15335 367850..368131 + 282 WP_001894429.1 DUF1289 domain-containing protein -
MS6_RS15345 368389..368925 + 537 WP_000469482.1 GNAT family N-acetyltransferase -
MS6_RS15350 369052..369567 + 516 WP_001201509.1 lipocalin family protein -
MS6_RS15355 369717..370214 - 498 WP_000982260.1 GNAT family N-acetyltransferase Toxin
MS6_RS15360 370211..370483 - 273 WP_000246253.1 DUF1778 domain-containing protein Antitoxin
MS6_RS19510 370876..371001 + 126 WP_001900195.1 DUF645 family protein -
MS6_RS20130 371017..371055 + 39 WP_106019118.1 hypothetical protein -
MS6_RS15375 371251..371697 + 447 WP_000006157.1 hypothetical protein -
MS6_RS15380 371845..372114 - 270 WP_000277238.1 type II toxin-antitoxin system YafQ family toxin -
MS6_RS15385 372107..372385 - 279 WP_001258569.1 type II toxin-antitoxin system RelB/DinJ family antitoxin -
MS6_RS15390 372635..372892 + 258 WP_000861987.1 type II toxin-antitoxin system Phd/YefM family antitoxin -
MS6_RS15395 372880..373182 + 303 WP_000229318.1 type II toxin-antitoxin system RelE/ParE family toxin -
MS6_RS19515 373471..373695 + 225 WP_001894423.1 DUF3709 domain-containing protein -
MS6_RS19520 374139..374258 + 120 WP_071919600.1 DUF645 family protein -
MS6_RS20145 374262..374315 + 54 Protein_369 DUF645 family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island qnrVC4 - 297729..445581 147852
- inside Integron qnrVC4 - 302809..445205 142396


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(86-152)

Antitoxin

Vibrio phage ICP1, Orf50

(7-86)


Sequences

Toxin        


Download         Length: 166 a.a.        Molecular weight: 18527.18 Da        Isoelectric Point: 8.7753

>T30638 WP_000982260.1 NZ_AP014525:c370214-369717 [Vibrio cholerae MS6]
MMNTVLLDKDKHDRNRFNCGTEALNNYLKVMASQQAKKDNTRTFVLEDDNNSAYIIGFYTLTMTPIDLKALPDKLQKKHQ
SSTSGGLIARLAVDDRYKGKGFGEWLLIDALRKLLAASDSVAFPVVIVDAKDGAKHFYERYGFQAFQDAENKLFITIADI
RASLG

Download         Length: 498 bp

>T30638 NZ_AP014525:c370214-369717 [Vibrio cholerae MS6]
ATGATGAATACGGTACTTCTCGATAAAGATAAGCACGATAGGAACCGTTTCAATTGTGGCACTGAAGCACTCAACAATTA
CTTAAAAGTAATGGCGAGTCAGCAAGCCAAAAAAGACAACACCAGAACCTTCGTTTTGGAAGATGACAATAACAGTGCTT
ATATCATCGGCTTTTACACTCTGACTATGACGCCCATTGATTTGAAAGCCTTGCCCGACAAGTTACAAAAGAAGCACCAA
TCCTCAACCTCTGGAGGGCTCATTGCTCGTTTAGCTGTCGACGATAGATACAAAGGAAAAGGTTTTGGTGAGTGGCTACT
CATTGACGCTCTCAGAAAGCTACTCGCAGCCAGTGACAGTGTAGCGTTCCCTGTTGTCATTGTTGATGCCAAAGATGGTG
CAAAACATTTCTATGAGCGCTATGGGTTTCAAGCGTTTCAAGATGCCGAAAACAAACTCTTCATCACCATTGCTGATATC
AGAGCAAGCTTAGGCTAG

Antitoxin


Download         Length: 91 a.a.        Molecular weight: 9683.22 Da        Isoelectric Point: 6.2988

>AT30638 WP_000246253.1 NZ_AP014525:c370483-370211 [Vibrio cholerae MS6]
MATTLPRITARVDVDTQDLLAKAAALAGMSSINSFVLNAAIEKAKQVIEREQALKLSQADAVLLMEALDNPAVVNAKLKL
ASERYESKTQ

Download         Length: 273 bp

>AT30638 NZ_AP014525:c370483-370211 [Vibrio cholerae MS6]
ATGGCAACGACTTTGCCTCGCATCACCGCTAGAGTTGATGTCGATACTCAAGATTTACTCGCTAAGGCTGCTGCTCTTGC
TGGTATGTCTAGTATTAACTCATTTGTTTTGAATGCAGCAATCGAAAAAGCAAAACAAGTCATTGAACGTGAGCAAGCTC
TAAAGCTTAGCCAAGCTGATGCCGTATTGCTGATGGAAGCTCTTGATAATCCAGCTGTAGTAAATGCAAAGCTCAAATTG
GCATCCGAACGTTATGAGAGCAAAACTCAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0Q0KGB1

Antitoxin

Source ID Structure
PDB 1Y9B
AlphaFold DB A0A0F2IC79

References