Detailed information of TA system TA030636

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): parDE/ParE-RHH
• Location: 347623..348161
• Replicon: chromosome
• Accession: NZ_AP014525
• Organism: Vibrio cholerae MS6

Toxin (Protein)

• Gene name: parE
• Uniprot ID: A0A0K9UM73
• Locus tag: MS6_RS15200
• Protein ID: WP_000802138.1
• Coordinates: 347623..347922 (-)
• Length: 100 a.a.

Antitoxin (Protein)

• Gene name: parD
• Uniprot ID: A0A271VK51
• Locus tag: MS6_RS15205
• Protein ID: WP_001107720.1
• Coordinates: 347919..348161 (-)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MS6_RS15165	342893..343330	+	438	WP_001894912.1	hypothetical protein	-
MS6_RS15170	343480..343731	+	252	WP_001128284.1	hypothetical protein	-
MS6_RS15175	343916..344431	+	516	WP_001894921.1	lipocalin family protein	-
MS6_RS15180	344591..345214	+	624	WP_000247072.1	DUF1349 domain-containing protein	-
MS6_RS15185	345371..345805	+	435	WP_000453680.1	GNAT family N-acetyltransferase	-
MS6_RS15190	345998..346909	+	912	WP_000174843.1	hypothetical protein	-
MS6_RS15195	347054..347506	+	453	WP_000863110.1	GNAT family N-acetyltransferase	-
MS6_RS15200	347623..347922	-	300	WP_000802138.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
MS6_RS15205	347919..348161	-	243	WP_001107720.1	type II toxin-antitoxin system ParD family antitoxin	Antitoxin
MS6_RS15210	348428..349141	+	714	WP_000123660.1	Fic family protein	-
MS6_RS15215	349329..349844	+	516	WP_000284463.1	hypothetical protein	-
MS6_RS15220	349963..350811	+	849	WP_000472050.1	hypothetical protein	-
MS6_RS20090	350796..350924	+	129	WP_088126095.1	DUF3265 domain-containing protein	-
MS6_RS19410	351341..351457	+	117	WP_080314581.1	DUF3265 domain-containing protein	-
MS6_RS15235	351733..352200	+	468	WP_016711073.1	hypothetical protein	-
MS6_RS15240	352639..352818	+	180	WP_001894916.1	DUF645 family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	qnrVC4	-	297729..445581	147852
	-	inside	Integron	qnrVC4	-	302809..445205	142396

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (6-92)

Antitoxin

• Antitoxin ParD (1-79)

Sequences

Toxin

Protein

• Length: 100 a.a.
• Molecular weight: 11617.34 Da
• Isoelectric Point: 9.8184

>T30636 WP_000802138.1 NZ_AP014525:c347922-347623 [Vibrio cholerae MS6]
MKPFNLTVAAKADLRDIALFTQRRWGKEQRNVYLKQFDDSFWLLAENPDIGKSCDEIREGYRKYPQGSHVIFYQQIGSQQ
IRVIRILHKSMDVNPIFGA

Nucleotide

• Length: 300 bp

>T30636 NZ_AP014525:c347922-347623 [Vibrio cholerae MS6]
ATGAAACCATTTAATCTTACTGTCGCCGCTAAAGCCGATTTACGGGATATTGCTTTATTCACTCAACGACGCTGGGGAAA
AGAGCAGCGAAATGTTTATTTAAAGCAATTCGATGATTCCTTTTGGCTTTTAGCGGAAAATCCCGACATCGGTAAATCAT
GCGATGAAATTCGAGAGGGATACAGAAAATATCCCCAAGGGAGCCACGTCATCTTTTATCAGCAAATCGGCAGCCAACAA
ATCAGGGTGATCCGAATTCTTCATAAGAGCATGGATGTGAACCCAATATTTGGCGCATAA

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 8950.82 Da
• Isoelectric Point: 4.1677

>AT30636 WP_001107720.1 NZ_AP014525:c348161-347919 [Vibrio cholerae MS6]
MAKNTSITLGEHFDGFITSQIQSGRYGSASEVIRSALRLLENQETKLQSLRQLLVEGEQSGDADYDLDSFINELDSENIR

Nucleotide

• Length: 243 bp

>AT30636 NZ_AP014525:c348161-347919 [Vibrio cholerae MS6]
ATGGCTAAAAACACAAGTATCACTCTTGGTGAACACTTCGATGGCTTTATTACAAGCCAAATACAAAGTGGACGTTACGG
CTCAGCAAGTGAAGTCATTCGCTCTGCGCTACGTCTACTCGAAAACCAAGAAACCAAACTACAGTCACTCCGTCAATTAC
TTGTTGAAGGTGAGCAAAGTGGTGATGCTGATTATGACCTTGATAGCTTCATCAATGAACTCGATAGTGAAAACATTCGA
TGA

Structures

Toxin

• AlphaFold DB: A0A0K9UM73

Antitoxin

• AlphaFold DB: A0A271VK51