Detailed information of TA system TA030635

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-YafN
• Location: 333363..333928
• Replicon: chromosome
• Accession: NZ_AP014525
• Organism: Vibrio cholerae MS6

Toxin (Protein)

• Gene name: relE
• Uniprot ID: D7HCW8
• Locus tag: MS6_RS15100
• Protein ID: WP_000869999.1
• Coordinates: 333641..333928 (+)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: D0IIK3
• Locus tag: MS6_RS15095
• Protein ID: WP_000086649.1
• Coordinates: 333363..333644 (+)
• Length: 94 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MS6_RS15060	328377..328835	+	459	WP_041051874.1	hypothetical protein	-
MS6_RS15065	328978..329595	+	618	WP_041051877.1	hypothetical protein	-
MS6_RS18845	329737..330138	+	402	WP_052469078.1	hypothetical protein	-
MS6_RS15075	330275..330853	+	579	WP_033930519.1	DJ-1/PfpI family protein	-
MS6_RS15080	331057..331446	+	390	WP_000491261.1	VOC family protein	-
MS6_RS15085	331792..332448	+	657	WP_000361703.1	QnrVC family quinolone resistance pentapeptide repeat protein	-
MS6_RS15090	332626..333162	+	537	WP_041051883.1	GNAT family N-acetyltransferase	-
MS6_RS15095	333363..333644	+	282	WP_000086649.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
MS6_RS15100	333641..333928	+	288	WP_000869999.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
MS6_RS15105	334086..334847	+	762	WP_001205114.1	hypothetical protein	-
MS6_RS20070	334912..335004	+	93	WP_108254096.1	iron transporter	-
MS6_RS15110	334974..335489	+	516	WP_000090012.1	hypothetical protein	-
MS6_RS20380	335840..335935	+	96	WP_001907607.1	DUF645 family protein	-
MS6_RS15115	336140..337039	+	900	WP_001887493.1	hypothetical protein	-
MS6_RS15125	337229..337615	+	387	WP_041051893.1	hypothetical protein	-
MS6_RS15130	337903..338085	+	183	WP_000923182.1	DUF645 family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	qnrVC4	-	297729..445581	147852
	-	inside	Integron	qnrVC4	-	302809..445205	142396

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (3-88)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (9-74)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 10997.74 Da
• Isoelectric Point: 6.0710

>T30635 WP_000869999.1 NZ_AP014525:333641-333928 [Vibrio cholerae MS6]
MKVVWSPLALQKLGDAAEFIALDNPSAAEKWVNEVFDKTELLGSMPEMGRMVPEMPHTNYREIIFGHYRIIYSLSHEIRV
LTVRNCRQLLTEHDV

Nucleotide

• Length: 288 bp

>T30635 NZ_AP014525:333641-333928 [Vibrio cholerae MS6]
ATGAAAGTAGTTTGGTCACCTCTAGCGTTACAAAAACTGGGTGATGCCGCAGAGTTTATTGCTTTGGATAACCCATCAGC
TGCAGAAAAGTGGGTGAATGAAGTATTCGACAAAACGGAATTGCTCGGCTCAATGCCAGAAATGGGCCGCATGGTTCCTG
AAATGCCTCATACGAACTACCGTGAAATAATTTTTGGTCATTACCGCATTATTTATAGTTTGAGCCACGAAATCCGCGTT
CTAACAGTTCGTAACTGTCGTCAATTGCTCACGGAACATGATGTGTGA

Antitoxin

Protein

• Length: 94 a.a.
• Molecular weight: 10361.93 Da
• Isoelectric Point: 5.1548

>AT30635 WP_000086649.1 NZ_AP014525:333363-333644 [Vibrio cholerae MS6]
MSRIHLDQDIQPLSEFRAGVASFIKQINETRRPLVITQRGKGVAVVLDVAEYEAMQEKIELLEEMRTAEAQLAAGLGISN
EDARSQVLGRIIK

Nucleotide

• Length: 282 bp

>AT30635 NZ_AP014525:333363-333644 [Vibrio cholerae MS6]
ATGAGCCGTATTCACCTTGATCAAGATATTCAGCCTTTGTCTGAGTTCCGTGCTGGCGTTGCATCATTTATCAAACAGAT
CAATGAGACTCGTCGACCATTGGTTATTACACAACGAGGTAAAGGTGTAGCAGTTGTTCTTGACGTTGCGGAGTATGAAG
CAATGCAAGAGAAAATCGAATTACTCGAAGAAATGCGTACTGCAGAAGCTCAATTGGCTGCAGGTTTAGGTATATCAAAC
GAAGATGCTCGTTCACAAGTTCTGGGGCGCATCATCAAATGA

Structures

Toxin

• AlphaFold DB: A0A0H3AEF1

Antitoxin

• AlphaFold DB: A0A0K9UIW4