Detailed information of TA system TA030627

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicA-HicB
• Location: 295651..296264
• Replicon: chromosome
• Accession: NZ_AP014522
• Organism: Pseudomonas protegens Cab57

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: A0A7U9LBG9
• Locus tag: PPC_RS01330
• Protein ID: WP_041115175.1
• Coordinates: 295651..295833 (+)
• Length: 61 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: PPC_RS01335
• Protein ID: WP_171820684.1
• Coordinates: 295830..296264 (+)
• Length: 145 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
PPC_RS01310	291390..293351	+	1962	WP_161466434.1	choline transporter BetT	-
PPC_RS01315	293361..293618	-	258	WP_041115171.1	hypothetical protein	-
PPC_RS01320	293671..294411	-	741	WP_019094920.1	SDR family oxidoreductase	-
PPC_RS01325	294543..295433	+	891	WP_011058633.1	LysR family transcriptional regulator	-
PPC_RS01330	295651..295833	+	183	WP_041115175.1	type II toxin-antitoxin system HicA family toxin	Toxin
PPC_RS01335	295830..296264	+	435	WP_171820684.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
PPC_RS01340	296277..297323	-	1047	WP_041115177.1	LacI family DNA-binding transcriptional regulator	-
PPC_RS01345	297529..299787	+	2259	WP_041115179.1	TonB-dependent receptor	-
PPC_RS01350	299931..301259	+	1329	WP_041115181.1	LLM class flavin-dependent oxidoreductase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (3-59)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (14-138)

Sequences

Toxin

Protein

• Length: 61 a.a.
• Molecular weight: 6866.97 Da
• Isoelectric Point: 10.8547

>T30627 WP_041115175.1 NZ_AP014522:295651-295833 [Pseudomonas protegens Cab57]
MRSREMISRIEADGWYLVAVKGSHHQYKHPFKPGRVTIRHPDTDLPKGTLNSILKQAGLK

Nucleotide

• Length: 183 bp

>T30627 NZ_AP014522:295651-295833 [Pseudomonas protegens Cab57]
ATGCGCAGTCGGGAAATGATCAGCAGGATCGAGGCGGATGGTTGGTACTTGGTGGCCGTCAAGGGCAGTCACCATCAGTA
CAAGCATCCTTTCAAGCCGGGCCGCGTGACCATCAGGCACCCGGATACGGATCTGCCCAAAGGCACGCTCAACAGCATCT
TGAAACAGGCGGGCCTGAAATGA

Antitoxin

Protein

• Length: 145 a.a.
• Molecular weight: 16003.82 Da
• Isoelectric Point: 4.3449

>AT30627 WP_171820684.1 NZ_AP014522:295830-296264 [Pseudomonas protegens Cab57]
MSLFLNEESNDMKFPVVLHKDADSEYGVTVPDVPGCHSAGPTVSLAFENIKEALSLHYEGLVADGEPLPQVHEIDDHLDN
PDYAGGVWGVVEFDVTPYFGKSVRFNATLPEQLLERIDQRVERDQRYRSRSGFLAAAALRELSA

Nucleotide

• Length: 435 bp

>AT30627 NZ_AP014522:295830-296264 [Pseudomonas protegens Cab57]
ATGAGCCTGTTTCTGAACGAGGAGTCCAACGACATGAAATTCCCGGTGGTTCTGCACAAGGATGCCGACTCCGAATATGG
GGTGACAGTTCCGGATGTACCGGGCTGCCATTCTGCCGGCCCGACGGTTTCCTTGGCGTTCGAGAACATCAAGGAAGCGC
TGTCCCTGCACTACGAGGGCCTGGTGGCGGACGGCGAGCCCTTGCCCCAGGTGCATGAGATTGACGATCACCTGGACAAC
CCCGACTACGCCGGTGGCGTATGGGGCGTGGTGGAGTTCGACGTGACGCCCTACTTCGGCAAGTCGGTGCGCTTCAACGC
CACCCTGCCGGAGCAATTGCTGGAGCGCATCGACCAGAGGGTCGAACGCGATCAGCGCTACCGTTCGCGTTCCGGGTTCC
TCGCCGCTGCGGCGCTGCGCGAGCTCTCTGCCTGA

Structures

Toxin

• AlphaFold DB: A0A7U9LBG9