Detailed information of TA system TA030622

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relE-PumB/upstrm_HI1419-dnstrm_HI1420
• Location: 1765722..1766319
• Replicon: chromosome
• Accession: NZ_AP014520
• Organism: Aggregatibacter actinomycetemcomitans NUM4039 strain NUM 4039

Toxin (Protein)

• Gene name: relE
• Uniprot ID: A0A142FXE8
• Locus tag: AANUM_RS08770
• Protein ID: WP_005595366.1
• Coordinates: 1765722..1766024 (+)
• Length: 101 a.a.

Antitoxin (Protein)

• Gene name: PumB
• Uniprot ID: G4A710
• Locus tag: AANUM_RS08775
• Protein ID: WP_005556438.1
• Coordinates: 1766026..1766319 (+)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AANUM_RS08750 (AANUM_1769)	1761032..1761780	-	749	Protein_1751	hypothetical protein	-
AANUM_RS13160 (AANUM_1770)	1761784..1762182	-	399	WP_005550363.1	hypothetical protein	-
AANUM_RS12345 (AANUM_1771)	1762131..1762319	-	189	WP_236757821.1	glycoside hydrolase family 19	-
AANUM_RS13165 (AANUM_1772)	1762362..1763357	-	996	WP_060828926.1	hypothetical protein	-
AANUM_RS08765 (AANUM_1773)	1763623..1765569	-	1947	WP_005538140.1	ABC transporter ATP-binding protein	-
AANUM_RS08770 (AANUM_1774)	1765722..1766024	+	303	WP_005595366.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
AANUM_RS08775 (AANUM_1775)	1766026..1766319	+	294	WP_005556438.1	putative addiction module antidote protein	Antitoxin
AANUM_RS08780 (AANUM_1776)	1766356..1767708	-	1353	WP_005538151.1	anti-phage deoxyguanosine triphosphatase	-
AANUM_RS08785	1767710..1768402	-	693	WP_005538152.1	CidB/LrgB family autolysis modulator	-
AANUM_RS12355	1768409..1768813	-	405	Protein_1760	CidA/LrgA family protein	-
AANUM_RS08795 (AANUM_1778)	1769388..1770389	+	1002	WP_005538156.1	site-specific integrase	-
AANUM_RS13170 (AANUM_1779)	1770498..1770677	-	180	WP_005538158.1	helix-turn-helix domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	1765722..1774939	9217

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (1-97)

Antitoxin

• Cro/C1-type helix-turn-helix domain (47-89)

Sequences

Toxin

Protein

• Length: 101 a.a.
• Molecular weight: 11413.93 Da
• Isoelectric Point: 5.8716

>T30622 WP_005595366.1 NZ_AP014520:1765722-1766024 [Aggregatibacter actinomycetemcomitans NUM4039]
MYIIEETEEFSNWLENLTDELAIVNIVARIERAKLGNFGDHKSVGNGVYEMRITKGKGYRIYYARKGGITYLLILGGDKS
SQEQDIQNAKALWQQIKNGE

Nucleotide

• Length: 303 bp

>T30622 NZ_AP014520:1765722-1766024 [Aggregatibacter actinomycetemcomitans NUM4039]
ATGTATATAATTGAGGAAACGGAGGAATTCTCCAACTGGTTAGAGAATCTAACCGACGAACTTGCGATTGTGAATATTGT
TGCTCGAATCGAAAGGGCAAAGCTCGGCAATTTCGGTGATCATAAATCGGTGGGTAATGGCGTATATGAAATGCGAATTA
CAAAAGGGAAAGGCTATAGGATCTACTATGCCAGAAAAGGGGGTATTACTTATCTATTGATTCTTGGTGGCGATAAGTCA
AGCCAAGAACAAGATATTCAGAATGCAAAAGCACTATGGCAACAGATTAAAAACGGAGAATAA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 10694.39 Da
• Isoelectric Point: 6.3545

>AT30622 WP_005556438.1 NZ_AP014520:1766026-1766319 [Aggregatibacter actinomycetemcomitans NUM4039]
MTVTTREFDAARYLKNEKMMAMYLETVLEEGTTAEFIQALNTVARAKGMSELAEKTGIGRESLYKTLSSEKPRFDTILKI
ISALGLSLSINAKDAVA

Nucleotide

• Length: 294 bp

>AT30622 NZ_AP014520:1766026-1766319 [Aggregatibacter actinomycetemcomitans NUM4039]
ATGACAGTAACAACCCGAGAATTTGATGCAGCGCGTTATTTGAAAAATGAAAAAATGATGGCAATGTATCTTGAAACAGT
TTTAGAAGAAGGCACAACGGCTGAGTTTATACAAGCTCTGAATACGGTCGCAAGAGCAAAGGGCATGTCGGAACTGGCGG
AAAAAACCGGCATCGGACGTGAAAGTTTGTACAAAACCCTATCAAGTGAAAAACCACGTTTTGACACGATTCTAAAAATT
ATTTCAGCATTGGGGCTTAGCCTTTCTATTAATGCTAAAGATGCAGTAGCCTGA

Structures

Toxin

• AlphaFold DB: A0A142FXE8

Antitoxin

• AlphaFold DB: A0A142FXE9