Detailed information of TA system TA030604

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazF-pemI/MazF(toxin)
• Location: 482676..483294
• Replicon: chromosome
• Accession: NZ_AP013294
• Organism: Bacillus sp. OxB-1

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: A0A0A8JGU4
• Locus tag: OXB_RS02570
• Protein ID: WP_041071628.1
• Coordinates: 482676..483026 (-)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: pemI
• Uniprot ID: -
• Locus tag: OXB_RS02575
• Protein ID: WP_041076189.1
• Coordinates: 483031..483294 (-)
• Length: 88 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
OXB_RS02530	477681..478277	-	597	WP_052483840.1	SpoIIE family protein phosphatase	-
OXB_RS02535	478277..479062	-	786	WP_041071617.1	RNA polymerase sigma factor SigB	-
OXB_RS02540	479037..479510	-	474	WP_041071619.1	anti-sigma B factor RsbW	-
OXB_RS02545	479497..479835	-	339	WP_041071621.1	anti-sigma factor antagonist	-
OXB_RS02550	479889..480923	-	1035	WP_041071623.1	PP2C family protein-serine/threonine phosphatase	-
OXB_RS02555	480938..481339	-	402	WP_041071625.1	anti-sigma regulatory factor	-
OXB_RS02560	481342..481707	-	366	WP_173425985.1	STAS domain-containing protein	-
OXB_RS02565	481704..482531	-	828	WP_041071627.1	STAS domain-containing protein	-
OXB_RS02570	482676..483026	-	351	WP_041071628.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
OXB_RS02575	483031..483294	-	264	WP_041076189.1	hypothetical protein	Antitoxin
OXB_RS02580	483455..484600	-	1146	WP_041071630.1	alanine racemase	-
OXB_RS02585	484818..485816	-	999	WP_041071632.1	outer membrane lipoprotein carrier protein LolA	-
OXB_RS02590	485892..486251	-	360	WP_041071634.1	holo-ACP synthase	-
OXB_RS02595	486321..486944	+	624	WP_041071635.1	rhomboid family intramembrane serine protease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (4-111)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12880.85 Da
• Isoelectric Point: 5.1663

>T30604 WP_041071628.1 NZ_AP013294:c483026-482676 [Bacillus sp. OxB-1]
MAIKRGDVFFADLSPVVGSEQGGTRPVLVIQNDIGNRFSPTVIIAAITAQIQKAKLPTHVEIDAARYGFERDSVILLEQV
RTIDKSRLTDKITQLDDHLMEKVDEALEISFGLVKF

Nucleotide

• Length: 351 bp

>T30604 NZ_AP013294:c483026-482676 [Bacillus sp. OxB-1]
TTGGCAATTAAACGTGGAGACGTCTTTTTTGCAGATCTGTCGCCTGTCGTCGGTTCAGAACAGGGAGGGACGAGACCGGT
CCTGGTCATCCAAAATGACATAGGAAACCGGTTCAGTCCGACAGTGATCATTGCAGCAATTACTGCGCAAATCCAAAAAG
CGAAATTGCCTACACATGTTGAAATCGATGCGGCGCGTTATGGTTTCGAGCGGGATTCGGTTATTCTGCTCGAACAGGTC
CGCACGATCGACAAGTCGAGGCTGACTGATAAAATTACGCAGCTAGATGATCATTTGATGGAAAAGGTCGATGAAGCGTT
GGAGATAAGCTTTGGCCTCGTCAAATTTTGA

Antitoxin

Protein

• Length: 88 a.a.
• Molecular weight: 10183.61 Da
• Isoelectric Point: 5.1750

>AT30604 WP_041076189.1 NZ_AP013294:c483294-483031 [Bacillus sp. OxB-1]
MKEVIVKIPKRMLNENEHTVVHQEPERGDFVYISTRRYVSDYEADTIREEMMKGYVEMSQINLKIAAECLHVEFEAQHTV
ERLVSGG

Nucleotide

• Length: 264 bp

>AT30604 NZ_AP013294:c483294-483031 [Bacillus sp. OxB-1]
ATGAAGGAAGTCATTGTTAAGATTCCGAAACGGATGCTGAATGAAAATGAACATACGGTCGTCCATCAGGAGCCGGAACG
TGGTGATTTCGTTTATATTTCGACAAGGCGATATGTGTCTGACTACGAAGCGGACACGATCCGAGAAGAAATGATGAAGG
GCTATGTCGAAATGTCGCAAATCAATCTGAAAATTGCCGCGGAATGCTTGCATGTTGAGTTTGAAGCCCAGCATACGGTG
GAACGTCTCGTAAGCGGAGGATGA

Structures

Toxin

• AlphaFold DB: A0A0A8JGU4