Detailed information of TA system TA030572

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VapB
• Location: 1112534..1113098
• Replicon: chromosome
• Accession: NZ_AP012555
• Organism: Mycobacterium avium subsp. hominissuis TH135

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: A0A2A2ZLT9
• Locus tag: MAH_RS05425
• Protein ID: WP_023868517.1
• Coordinates: 1112727..1113098 (+)
• Length: 124 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: MAH_RS23740
• Protein ID: WP_011724021.1
• Coordinates: 1112534..1112740 (+)
• Length: 69 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MAH_RS05405	1107788..1108786	+	999	WP_023868514.1	4-hydroxy-3-methylbut-2-enyl diphosphate reductase	-
MAH_RS05410	1108810..1110018	-	1209	WP_179145049.1	hypothetical protein	-
MAH_RS05415	1110149..1111222	+	1074	WP_044543554.1	redox-regulated ATPase YchF	-
MAH_RS05420	1111422..1112366	+	945	WP_044543555.1	HNH endonuclease	-
MAH_RS23740	1112534..1112740	+	207	WP_011724021.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
MAH_RS05425	1112727..1113098	+	372	WP_023868517.1	type II toxin-antitoxin system VapC family toxin	Toxin
MAH_RS05430	1113198..1113638	+	441	WP_003875392.1	nitroreductase family deazaflavin-dependent oxidoreductase	-
MAH_RS05435	1113704..1114441	-	738	WP_044543556.1	SDR family oxidoreductase	-
MAH_RS05440	1114516..1115121	+	606	WP_095975041.1	TetR/AcrR family transcriptional regulator; helix-turn-helix transcriptional regulator	-
MAH_RS05445	1115223..1115621	+	399	WP_044543557.1	VOC family protein	-
MAH_RS23745	1115690..1116073	+	384	WP_011724026.1	hypothetical protein	-
MAH_RS05450	1116143..1116496	+	354	WP_023865350.1	hypothetical protein	-
MAH_RS05455	1116653..1117261	+	609	WP_080772802.1	glycoside hydrolase	-
MAH_RS05460	1117627..1117950	+	324	WP_044543559.1	antibiotic biosynthesis monooxygenase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	1105620..1113638	8018

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-112)

Antitoxin

• Bacterial antitoxin of type II TA system, VapB (1-42)

Sequences

Toxin

Protein

• Length: 124 a.a.
• Molecular weight: 13754.08 Da
• Isoelectric Point: 6.6032

>T30572 WP_023868517.1 NZ_AP012555:1112727-1113098 [Mycobacterium avium subsp. hominissuis TH135]
MILVDTSVWIDHLHVADKRLIEFLTNDAIGCHQMVIEELALGAIRRRADLLRLLSNLRAFPLLTHAEVLHLVESHRLWGR
GLSAIDVHLLGSVALVDGARLWTRDKNLKAAGWDVGIAIVDEL

Nucleotide

• Length: 372 bp

>T30572 NZ_AP012555:1112727-1113098 [Mycobacterium avium subsp. hominissuis TH135]
ATGATTCTCGTTGACACGTCGGTATGGATCGATCATCTGCACGTCGCCGACAAGCGACTCATAGAGTTCCTGACCAACGA
CGCAATCGGTTGCCACCAAATGGTGATCGAGGAGTTGGCATTGGGTGCGATCAGGCGGCGCGCCGATCTATTGCGCCTGC
TGTCCAATCTTCGGGCGTTCCCGTTGCTGACGCATGCGGAGGTCCTTCATCTCGTCGAAAGCCACCGCTTGTGGGGCCGC
GGACTGAGTGCGATCGACGTCCATCTTCTTGGGTCGGTTGCTCTGGTCGACGGTGCACGGCTATGGACCAGGGACAAGAA
TTTAAAAGCGGCGGGCTGGGACGTGGGTATCGCGATCGTCGACGAGCTCTGA

Antitoxin

Protein

• Length: 69 a.a.
• Molecular weight: 7261.25 Da
• Isoelectric Point: 11.0634

>AT30572 WP_011724021.1 NZ_AP012555:1112534-1112740 [Mycobacterium avium subsp. hominissuis TH135]
MRTTVTIDDALLAEAAELTGVTESVALLRQGLQTLVRVESARRLAALGGTDRKASVGPRRRTPADDSR

Nucleotide

• Length: 207 bp

>AT30572 NZ_AP012555:1112534-1112740 [Mycobacterium avium subsp. hominissuis TH135]
ATGAGGACGACGGTGACGATTGACGACGCCTTATTGGCAGAGGCGGCCGAACTGACCGGGGTGACAGAGAGCGTGGCGCT
CCTCCGTCAGGGACTGCAGACACTCGTTCGGGTAGAGAGCGCCCGTCGGCTGGCGGCGCTCGGCGGCACGGATCGGAAGG
CATCGGTCGGCCCTCGCCGGAGGACACCCGCCGATGATTCTCGTTGA

Structures

Toxin

• AlphaFold DB: A0A2A2ZLT9