Detailed information of TA system TA030571

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): unclassified/Polyketide_cyc2(toxin)
• Location: 885756..886409
• Replicon: chromosome
• Accession: NZ_AP012555
• Organism: Mycobacterium avium subsp. hominissuis TH135

Toxin (Protein)

• Gene name: novel[antitoxin]
• Uniprot ID: -
• Locus tag: MAH_RS04380
• Protein ID: WP_003872880.1
• Coordinates: 885948..886409 (+)
• Length: 154 a.a.

Antitoxin (Protein)

• Gene name: novel[toxin]
• Uniprot ID: A0A808C2V3
• Locus tag: MAH_RS04375
• Protein ID: WP_023869662.1
• Coordinates: 885756..885944 (+)
• Length: 63 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MAH_RS04365	881738..883324	+	1587	WP_023869664.1	serine hydrolase	-
MAH_RS04370	883321..885693	+	2373	WP_023869663.1	cation-translocating P-type ATPase	-
MAH_RS04375	885756..885944	+	189	WP_023869662.1	antitoxin	Antitoxin
MAH_RS04380	885948..886409	+	462	WP_003872880.1	SRPBCC family protein	Toxin
MAH_RS04385	886478..886912	+	435	WP_023869661.1	hypothetical protein	-
MAH_RS04390	886917..887393	-	477	WP_023869660.1	VOC family protein	-
MAH_RS04395	888358..889941	+	1584	WP_003872878.1	DUF4185 domain-containing protein	-
MAH_RS04400	889988..890374	+	387	WP_023869659.1	hypothetical protein	-
MAH_RS23700	890409..890627	-	219	WP_023869658.1	hypothetical protein	-
MAH_RS23705	890818..891392	-	575	Protein_881	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Polyketide cyclase/dehydrase (2-142)

Antitoxin

• Antitoxin Rv0909/MT0933 (4-51)

Sequences

Toxin

Protein

• Length: 154 a.a.
• Molecular weight: 16626.19 Da
• Isoelectric Point: 9.5654

>T30571 WP_003872880.1 NZ_AP012555:885948-886409 [Mycobacterium avium subsp. hominissuis TH135]
VAKLSGSIDVPLPPEVAWQHASDLSRYKEWLTIHRVWRSTLPDQIDKGTVVESIVEVKGMLNRIKWTVVRYKPPEGMTLN
GDGVGGVKVKLMAKVQPKADGSVVSFDVHLGGPALFGPIGMIVAAALRGDIDQSLENFVTVFARSDPSTNGHR

Nucleotide

• Length: 462 bp

>T30571 NZ_AP012555:885948-886409 [Mycobacterium avium subsp. hominissuis TH135]
GTGGCCAAGCTATCCGGATCCATCGATGTTCCGCTGCCGCCCGAGGTGGCCTGGCAGCATGCCTCCGACCTGTCCCGGTA
CAAGGAGTGGCTGACCATTCACCGGGTGTGGCGCAGCACCTTGCCCGACCAGATCGACAAGGGCACCGTCGTCGAGTCGA
TCGTCGAGGTCAAGGGCATGCTCAACCGGATCAAATGGACGGTGGTGCGCTACAAGCCACCGGAGGGCATGACGCTCAAC
GGCGACGGCGTCGGCGGGGTCAAGGTCAAGCTGATGGCCAAGGTGCAGCCCAAGGCGGACGGTTCCGTCGTCAGCTTCGA
CGTGCACCTCGGCGGTCCGGCGCTGTTCGGGCCCATCGGCATGATCGTCGCGGCCGCGCTGCGCGGCGACATCGACCAGT
CGCTGGAGAACTTCGTCACGGTGTTCGCCCGATCGGATCCGAGCACCAACGGTCATCGCTGA

Antitoxin

Protein

• Length: 63 a.a.
• Molecular weight: 6729.36 Da
• Isoelectric Point: 4.4702

>AT30571 WP_023869662.1 NZ_AP012555:885756-885944 [Mycobacterium avium subsp. hominissuis TH135]
MGFLDKAKDLLSQNADKVEIAIDKAGEFVDDKTQGKYSDTIHQVQEQAKKATGSTETGDQQG

Nucleotide

• Length: 189 bp

>AT30571 NZ_AP012555:885756-885944 [Mycobacterium avium subsp. hominissuis TH135]
ATGGGATTCCTGGACAAGGCCAAGGACCTGTTGTCGCAGAACGCCGACAAGGTCGAGATTGCCATCGACAAGGCCGGCGA
GTTCGTCGACGACAAGACGCAGGGCAAGTACTCCGACACCATCCACCAGGTGCAGGAGCAGGCCAAGAAGGCCACCGGCA
GCACCGAGACCGGCGACCAGCAGGGCTGA

Structures

Antitoxin

• AlphaFold DB: A0A808C2V3