Detailed information of TA system TA030564

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ccdAB/CcdA(antitoxin)
• Location: 1235034..1235590
• Replicon: chromosome
• Accession: NZ_AP012547
• Organism: Sulfuritalea hydrogenivorans sk43H strain DSM 22779

Toxin (Protein)

• Gene name: ccdB
• Uniprot ID: W0SDZ6
• Locus tag: SUTH_RS05940
• Protein ID: WP_041097833.1
• Coordinates: 1235034..1235348 (-)
• Length: 105 a.a.

Antitoxin (Protein)

• Gene name: ccdA
• Uniprot ID: W0SD66
• Locus tag: SUTH_RS05945
• Protein ID: WP_041097835.1
• Coordinates: 1235348..1235590 (-)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
SUTH_RS05920	1231671..1232216	+	546	WP_041097827.1	ubiquinol-cytochrome c reductase iron-sulfur subunit	-
SUTH_RS05925	1232161..1233075	-	915	WP_084207274.1	DMT family transporter	-
SUTH_RS05930	1233089..1233349	-	261	WP_041097829.1	hypothetical protein	-
SUTH_RS05935	1233353..1235017	-	1665	WP_041097831.1	energy-dependent translational throttle protein EttA	-
SUTH_RS05940	1235034..1235348	-	315	WP_041097833.1	CcdB family protein	Toxin
SUTH_RS05945	1235348..1235590	-	243	WP_041097835.1	type II toxin-antitoxin system CcdA family antitoxin	Antitoxin
SUTH_RS18330	1235702..1236652	+	951	WP_052473317.1	hypothetical protein	-
SUTH_RS05955	1236653..1236922	-	270	WP_041097837.1	biotin/lipoyl-binding protein	-
SUTH_RS05960	1236931..1238376	-	1446	WP_084207275.1	tRNA 5-hydroxyuridine modification protein YegQ	-
SUTH_RS05965	1238271..1238954	-	684	WP_041097841.1	glutathione S-transferase family protein	-
SUTH_RS05970	1239189..1239713	-	525	WP_041097843.1	glycine cleavage system protein R	-
SUTH_RS05975	1239753..1240133	-	381	WP_041097845.1	type II toxin-antitoxin system VapC family toxin	-
SUTH_RS05980	1240133..1240345	-	213	WP_041097847.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin CcdB (5-103)

Antitoxin

• Post-segregation antitoxin CcdA (6-76)

Sequences

Toxin

Protein

• Length: 105 a.a.
• Molecular weight: 11316.08 Da
• Isoelectric Point: 4.4667

>T30564 WP_041097833.1 NZ_AP012547:c1235348-1235034 [Sulfuritalea hydrogenivorans sk43H]
MAHLDVFRNPDATTASVIPYVVDVQSELLADLPTHVVIPLAYPEAIETPILRLNPKVGVGDTALVVLTQDMASVSNRLLR
HPVANLSPQRDEILAALDFLFTGF

Nucleotide

• Length: 315 bp

>T30564 NZ_AP012547:c1235348-1235034 [Sulfuritalea hydrogenivorans sk43H]
ATGGCTCACCTCGACGTCTTTCGCAATCCGGATGCCACGACGGCAAGCGTCATCCCCTACGTAGTCGACGTCCAGAGCGA
ACTGCTCGCTGACCTGCCTACCCACGTCGTCATCCCCTTGGCCTATCCTGAAGCCATCGAGACCCCGATCCTGCGCCTCA
ACCCGAAAGTCGGCGTCGGCGACACGGCGTTGGTCGTATTGACACAGGACATGGCCTCGGTGTCAAACCGCCTGCTGCGA
CACCCCGTTGCCAACCTTTCCCCCCAGCGCGACGAGATCCTTGCCGCGCTGGATTTCCTTTTCACCGGTTTCTGA

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 9234.59 Da
• Isoelectric Point: 9.8330

>AT30564 WP_041097835.1 NZ_AP012547:c1235590-1235348 [Sulfuritalea hydrogenivorans sk43H]
MSAHPKKAANLTVRADLLEEARARKINLSQTLEVALSVELKKCRETEWLEQNKEAIAAYGRFVEKHGVFSDRFRNFMREG

Nucleotide

• Length: 243 bp

>AT30564 NZ_AP012547:c1235590-1235348 [Sulfuritalea hydrogenivorans sk43H]
ATGAGCGCCCATCCCAAGAAAGCCGCCAACCTCACCGTCCGCGCCGATCTGCTTGAAGAAGCCCGCGCGCGCAAGATCAA
CCTCTCGCAGACGCTCGAAGTCGCGCTTTCCGTCGAGCTGAAGAAGTGTCGCGAAACCGAGTGGCTGGAGCAGAACAAGG
AGGCCATTGCCGCCTACGGGCGTTTCGTCGAGAAGCACGGCGTGTTCTCCGACCGCTTCCGCAACTTCATGCGTGAGGGT
TGA

Structures

Toxin

• AlphaFold DB: W0SDZ6

Antitoxin

• AlphaFold DB: W0SD66