Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemIK/MazF(toxin)
Location 2648854..2649505 Replicon chromosome
Accession NZ_AP012541
Organism Lacticaseibacillus paracasei subsp. paracasei JCM 8130

Toxin (Protein)


Gene name pemK Uniprot ID K6S8L5
Locus tag LBPC_RS12985 Protein ID WP_003567661.1
Coordinates 2648854..2649237 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID K6RKI5
Locus tag LBPC_RS12990 Protein ID WP_003567665.1
Coordinates 2649257..2649505 (-) Length 83 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LBPC_RS12980 2648149..2648670 - 522 WP_003662697.1 QueT transporter family protein -
LBPC_RS12985 2648854..2649237 - 384 WP_003567661.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
LBPC_RS12990 2649257..2649505 - 249 WP_003567665.1 antitoxin Antitoxin
LBPC_RS12995 2649573..2650709 - 1137 WP_003662696.1 alanine racemase -
LBPC_RS13000 2650696..2651070 - 375 WP_003567669.1 holo-ACP synthase -
LBPC_RS13005 2651240..2652748 - 1509 WP_003567670.1 DEAD/DEAH box helicase -
LBPC_RS17090 2652874..2653017 - 144 WP_003567673.1 hypothetical protein -
LBPC_RS13015 2653048..2654436 - 1389 WP_003662693.1 UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D- alanine ligase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(6-112)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 13773.03 Da        Isoelectric Point: 10.9764

>T30561 WP_003567661.1 NZ_AP012541:c2649237-2648854 [Lacticaseibacillus paracasei subsp. paracasei JCM 8130]
MDVSVKRGDVFFADLSPVVGSEQGGNRPVLIIQNNVGNHYSPTVIVAAITSKIQKPKMPTHVGLRAKQDGVERNSVILLE
QIRTIDKQRLQARVTALSSTKMAAVDRALAISVGLVSLPKPKTYNKN

Download         Length: 384 bp

>T30561 NZ_AP012541:c2649237-2648854 [Lacticaseibacillus paracasei subsp. paracasei JCM 8130]
ATGGATGTGTCCGTAAAACGCGGCGATGTTTTTTTCGCCGATCTGTCTCCGGTTGTCGGCTCCGAACAAGGCGGCAACCG
GCCAGTACTTATTATTCAAAACAACGTTGGCAACCATTACAGTCCCACAGTGATTGTGGCAGCCATTACCTCCAAGATTC
AAAAACCCAAGATGCCGACGCATGTCGGGTTACGGGCCAAGCAAGATGGCGTTGAACGCAACTCGGTCATTTTACTTGAG
CAAATTCGCACGATTGACAAGCAACGGCTACAAGCCCGCGTGACAGCTCTGTCAAGCACCAAAATGGCAGCAGTCGACCG
CGCGTTGGCGATTTCAGTGGGTCTGGTCAGTTTGCCCAAGCCCAAAACATATAATAAGAATTAA

Antitoxin


Download         Length: 83 a.a.        Molecular weight: 8938.08 Da        Isoelectric Point: 4.4610

>AT30561 WP_003567665.1 NZ_AP012541:c2649505-2649257 [Lacticaseibacillus paracasei subsp. paracasei JCM 8130]
MKKQTSVAVTFDDAIKARLDAYCELNDIDAQAVITEAVATFLKSQAPEINQLISGYVAMGQINTEISHAFSACESEAYAH
IR

Download         Length: 249 bp

>AT30561 NZ_AP012541:c2649505-2649257 [Lacticaseibacillus paracasei subsp. paracasei JCM 8130]
ATGAAAAAGCAAACGTCAGTAGCGGTAACGTTTGATGATGCCATTAAGGCGCGGTTGGATGCCTATTGTGAATTGAATGA
CATTGACGCTCAGGCTGTGATTACGGAGGCAGTGGCGACATTTTTGAAGTCACAGGCTCCCGAAATCAACCAATTGATTT
CTGGCTATGTAGCAATGGGCCAGATCAATACAGAGATCAGTCATGCTTTCTCTGCCTGCGAGAGCGAGGCATACGCCCAT
ATTCGATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB K6S8L5

Antitoxin

Source ID Structure
AlphaFold DB A0A0E2BNY2

References