Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) spoIISABC/SpoIISA-SpoIISB
Location 1323680..1324596 Replicon chromosome
Accession NZ_AP012496
Organism Bacillus subtilis BEST7003

Toxin (Protein)


Gene name spoIISA Uniprot ID O34853
Locus tag BEST7003_RS06730 Protein ID WP_003244695.1
Coordinates 1323850..1324596 (-) Length 249 a.a.

Antitoxin (Protein)


Gene name spoIISB Uniprot ID G4EXD8
Locus tag BEST7003_RS06725 Protein ID WP_003232646.1
Coordinates 1323680..1323850 (-) Length 57 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BEST7003_RS06695 1320543..1320872 + 330 WP_003232660.1 phage-like element PBSX protein XkdW -
BEST7003_RS06700 1320869..1321033 + 165 WP_003232658.1 XkdX family protein -
BEST7003_RS06705 1321077..1321916 + 840 WP_003245597.1 phage-like element PBSX protein XepA -
BEST7003_RS06710 1321969..1322238 + 270 WP_003232655.1 hemolysin XhlA family protein -
BEST7003_RS06715 1322251..1322514 + 264 WP_003232653.1 phage holin -
BEST7003_RS06720 1322527..1323420 + 894 WP_003245230.1 N-acetylmuramoyl-L-alanine amidase -
BEST7003_RS21710 1323457..1323594 - 138 WP_003232648.1 three component toxin-antitoxin-antitoxin system antitoxin SpoIISC -
BEST7003_RS06725 1323680..1323850 - 171 WP_003232646.1 three component toxin-antitoxin-antitoxin system antitoxin SpoIISB Antitoxin
BEST7003_RS06730 1323850..1324596 - 747 WP_003244695.1 toxin-antitoxin-antitoxin system toxin SpoIISA Toxin
BEST7003_RS06735 1324706..1325707 - 1002 WP_003232642.1 inorganic phosphate transporter -
BEST7003_RS06740 1325720..1326337 - 618 WP_003218470.1 DUF47 domain-containing protein -
BEST7003_RS06745 1326613..1327929 - 1317 WP_003244819.1 serine/threonine exchanger -
BEST7003_RS06750 1328318..1329268 + 951 WP_003245086.1 ring-cleaving dioxygenase -
BEST7003_RS21715 1329369..1329515 + 147 WP_003244977.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin SpoIISA, type II toxin-antitoxin system

(3-238)

Antitoxin

Antitoxin SpoIISB

(1-56)


Sequences

Toxin        


Download         Length: 249 a.a.        Molecular weight: 29061.50 Da        Isoelectric Point: 4.6191

>T30552 WP_003244695.1 NZ_AP012496:c1324596-1323850 [Bacillus subtilis BEST7003]
MVLFFQIMVWCIVAGLGLYVYATWRFEAKVKEKMSAIRKTWYLLFVLGAMVYWTYEPTSLFTHWERYLIVAVSFALIDAF
IFLSAYVKKLAGSELETDTREILEENNEMLHMYLNRLKTYQYLLKNEPIHVYYGSIDAYAEGIDKLLKTYADKMNLTASL
CHYSTQADKDRLTEHMDDPADVQTRLDRKDVYYDQYGKVVLIPFTIETQNYVIKLTSDSIVTEFDYLLFTSLTSIYDLVL
PIEEEGEG

Download         Length: 747 bp

>T30552 NZ_AP012496:c1324596-1323850 [Bacillus subtilis BEST7003]
ATGGTTTTATTCTTTCAGATCATGGTCTGGTGCATCGTGGCCGGACTGGGGTTATACGTGTATGCCACGTGGCGTTTCGA
AGCGAAGGTCAAAGAAAAAATGTCCGCCATTCGGAAAACTTGGTATTTGCTGTTTGTTCTGGGCGCTATGGTATACTGGA
CATATGAGCCCACTTCCCTATTTACCCACTGGGAACGGTATCTCATTGTCGCAGTCAGTTTTGCTTTGATTGATGCTTTT
ATCTTCTTAAGTGCATATGTCAAAAAACTGGCCGGCAGCGAGCTTGAAACAGACACAAGAGAAATTCTTGAAGAAAACAA
CGAAATGCTCCACATGTATCTCAATCGGCTGAAAACATACCAATACCTATTGAAAAACGAACCGATCCATGTTTATTATG
GAAGTATAGATGCTTATGCTGAAGGTATTGATAAGCTGCTGAAAACCTATGCTGATAAAATGAACTTAACGGCTTCTCTT
TGCCACTATTCGACACAGGCTGATAAAGACCGGTTAACCGAGCATATGGATGATCCGGCAGATGTACAAACACGGCTCGA
TCGAAAGGATGTTTATTACGACCAATACGGAAAAGTGGTTCTCATCCCTTTTACCATCGAGACACAGAACTATGTCATCA
AGCTGACGTCTGACAGCATTGTCACGGAATTTGATTATTTGCTATTTACGTCATTAACGAGCATATATGATTTGGTGCTG
CCAATTGAGGAGGAAGGTGAAGGATAA

Antitoxin


Download         Length: 57 a.a.        Molecular weight: 6654.72 Da        Isoelectric Point: 11.0203

>AT30552 WP_003232646.1 NZ_AP012496:c1323850-1323680 [Bacillus subtilis BEST7003]
MERAFQNRCEPRAAKPFKILKKRSTTSVASYQVSPHTARIFKENERLIDEYKRKKA

Download         Length: 171 bp

>AT30552 NZ_AP012496:c1323850-1323680 [Bacillus subtilis BEST7003]
ATGGAACGTGCGTTTCAAAACAGATGCGAGCCCAGAGCGGCGAAGCCGTTTAAAATCCTGAAAAAACGTTCAACCACCAG
TGTCGCAAGCTATCAAGTCAGTCCGCATACAGCAAGAATCTTCAAAGAAAACGAACGGCTGATTGACGAGTATAAACGAA
AAAAAGCATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 3O6Q

Antitoxin

Source ID Structure
PDB 3O6Q
AlphaFold DB G4EXD8

References