Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 518401..519037 Replicon chromosome
Accession NZ_AP012496
Organism Bacillus subtilis BEST7003

Toxin (Protein)


Gene name mazF Uniprot ID G4NU33
Locus tag BEST7003_RS02580 Protein ID WP_003156187.1
Coordinates 518687..519037 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID G4NU32
Locus tag BEST7003_RS02575 Protein ID WP_003225183.1
Coordinates 518401..518682 (+) Length 94 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BEST7003_RS02555 514761..515360 - 600 WP_003225175.1 rhomboid family intramembrane serine protease -
BEST7003_RS02560 515455..515820 + 366 WP_003225177.1 holo-ACP synthase -
BEST7003_RS02565 515985..517001 + 1017 WP_080273356.1 outer membrane lipoprotein carrier protein LolA -
BEST7003_RS02570 517116..518285 + 1170 WP_003234284.1 alanine racemase -
BEST7003_RS02575 518401..518682 + 282 WP_003225183.1 type II toxin-antitoxin system antitoxin EndoAI Antitoxin
BEST7003_RS02580 518687..519037 + 351 WP_003156187.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
BEST7003_RS02585 519152..519976 + 825 WP_009966610.1 RsbT co-antagonist protein RsbRA -
BEST7003_RS02590 519981..520346 + 366 WP_003225190.1 RsbT antagonist protein RsbS -
BEST7003_RS02595 520350..520751 + 402 WP_003246640.1 serine/threonine-protein kinase RsbT -
BEST7003_RS02600 520763..521770 + 1008 WP_003234295.1 phosphoserine phosphatase RsbU -
BEST7003_RS02605 521832..522161 + 330 WP_003234298.1 anti-sigma factor antagonist RsbV -
BEST7003_RS02610 522158..522640 + 483 WP_003234299.1 anti-sigma B factor RsbW -
BEST7003_RS02615 522606..523394 + 789 WP_003246715.1 RNA polymerase sigma factor SigB -
BEST7003_RS02620 523394..523993 + 600 WP_003246608.1 phosphoserine phosphatase RsbX -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin

Ribbon-helix-helix protein, CopG

(12-45)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12977.98 Da        Isoelectric Point: 4.8781

>T30551 WP_003156187.1 NZ_AP012496:518687-519037 [Bacillus subtilis BEST7003]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Download         Length: 351 bp

>T30551 NZ_AP012496:518687-519037 [Bacillus subtilis BEST7003]
TTGATTGTGAAACGCGGCGATGTTTATTTTGCTGATTTATCTCCTGTTGTTGGCTCAGAGCAAGGCGGGGTGCGCCCGGT
TTTAGTGATCCAAAATGACATCGGAAATCGCTTCAGCCCAACTGCTATTGTTGCAGCCATAACAGCACAAATACAGAAAG
CGAAATTACCAACCCACGTCGAAATCGATGCAAAACGCTACGGTTTTGAAAGAGATTCCGTTATTTTGCTGGAGCAAATT
CGGACGATTGACAAGCAAAGGTTAACGGATAAGATTACTCATCTGGATGATGAAATGATGGATAAGGTTGATGAAGCCTT
ACAAATCAGTTTGGCACTCATTGATTTTTAG

Antitoxin


Download         Length: 94 a.a.        Molecular weight: 10546.92 Da        Isoelectric Point: 6.4708

>AT30551 WP_003225183.1 NZ_AP012496:518401-518682 [Bacillus subtilis BEST7003]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHFAEC
EAETTVERLVSGG

Download         Length: 282 bp

>AT30551 NZ_AP012496:518401-518682 [Bacillus subtilis BEST7003]
TTGTCTGAATCCAGCGCAAGAACCGAAATGAAAATCAGCTTGCCCGAAAACCTAGTAGCTGAATTGGATGGTGTAGCGAT
GCGGGAGAAACGAAGCAGAAACGAACTGATATCACAAGCAGTGAGAGCGTATGTCAGCGAACGAACAACTCGTCACAACC
GTGATTTGATGAGACGCGGCTATATGGAAATGGCGAAAATCAACCTGAATATTTCTTCTGAGGCTCACTTTGCGGAGTGC
GAGGCTGAAACGACAGTTGAGCGCTTAGTCAGCGGAGGATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4MDX
PDB 4ME7
PDB 1NE8

Antitoxin

Source ID Structure
PDB 4ME7
AlphaFold DB G4NU32

References