Detailed information of TA system TA030550

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): parDE/RelB(antitoxin)
• Location: 1580143..1580755
• Replicon: chromosome
• Accession: NZ_AP012335
• Organism: Streptococcus pyogenes JRS4

Toxin (Protein)

• Gene name: parE
• Uniprot ID: Q5X9X8
• Locus tag: SPYJRS4_RS07895
• Protein ID: WP_011018227.1
• Coordinates: 1580420..1580755 (+)
• Length: 112 a.a.

Antitoxin (Protein)

• Gene name: parD
• Uniprot ID: Q5X9X9
• Locus tag: SPYJRS4_RS07890
• Protein ID: WP_002988079.1
• Coordinates: 1580143..1580430 (+)
• Length: 96 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
SPYJRS4_RS07865	1575334..1576317	-	984	WP_011184964.1	tagatose-bisphosphate aldolase	-
SPYJRS4_RS07870	1576321..1577250	-	930	WP_011184965.1	tagatose-6-phosphate kinase	-
SPYJRS4_RS07875	1577296..1577811	-	516	WP_011184966.1	galactose-6-phosphate isomerase subunit LacB	-
SPYJRS4_RS07880	1577846..1578274	-	429	WP_002982747.1	galactose-6-phosphate isomerase subunit LacA	-
SPYJRS4_RS07885	1578720..1579493	+	774	WP_011184967.1	DeoR/GlpR family DNA-binding transcription regulator	-
SPYJRS4_RS07890	1580143..1580430	+	288	WP_002988079.1	DNA-damage-inducible protein J	Antitoxin
SPYJRS4_RS07895	1580420..1580755	+	336	WP_011018227.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
SPYJRS4_RS09550	1580853..1581891	+	1039	Protein_1510	site-specific integrase	-
SPYJRS4_RS07905	1582023..1582415	-	393	WP_002982716.1	30S ribosomal protein S9	-
SPYJRS4_RS07910	1582436..1582882	-	447	WP_011018228.1	50S ribosomal protein L13	-
SPYJRS4_RS07915	1583100..1583306	-	207	WP_002982695.1	helix-turn-helix transcriptional regulator	-
SPYJRS4_RS07920	1583303..1584001	-	699	WP_021340263.1	hypothetical protein	-
SPYJRS4_RS07925	1584137..1584997	-	861	WP_002982687.1	DegV family protein	-
SPYJRS4_RS07930	1585094..1585612	-	519	WP_011055010.1	NYN domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (8-105)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 112 a.a.
• Molecular weight: 13171.97 Da
• Isoelectric Point: 5.2144

>T30550 WP_011018227.1 NZ_AP012335:1580420-1580755 [Streptococcus pyogenes JRS4]
MDYKKYQIIYAPDVLEKLKEIRDYISQNYSSTSGQRKMEQIISDIEKLEVFPEVGFDADEKYGSKIIHYHSTKGYTLSKD
YIVLYHIEGEENRVVIDYLLPTQSDYIKLFK

Nucleotide

• Length: 336 bp

>T30550 NZ_AP012335:1580420-1580755 [Streptococcus pyogenes JRS4]
TTGGACTATAAGAAATATCAGATTATCTATGCTCCTGATGTTTTAGAGAAGCTAAAAGAAATTCGTGATTATATTTCTCA
AAACTATTCCTCAACATCAGGTCAGCGTAAAATGGAGCAAATCATTAGCGACATAGAAAAACTCGAGGTTTTCCCAGAAG
TTGGTTTTGATGCCGATGAAAAATATGGTTCAAAAATCATCCATTATCACAGCACTAAAGGTTATACCTTAAGTAAAGAT
TATATTGTCCTATACCATATCGAGGGGGAAGAAAATAGGGTCGTTATTGATTACTTGCTTCCTACTCAAAGCGACTATAT
AAAGTTATTCAAATAA

Antitoxin

Protein

• Length: 96 a.a.
• Molecular weight: 10934.56 Da
• Isoelectric Point: 5.2318

>AT30550 WP_002988079.1 NZ_AP012335:1580143-1580430 [Streptococcus pyogenes JRS4]
MTTVKKNRAVTFQANKELVSEAMTVLNKKNLTLSSALRLFLQNVVVTNEVDLLTEEELEKEKLFKQFQAEINKNIEDVRQ
GKFYTSEEVRAELGL

Nucleotide

• Length: 288 bp

>AT30550 NZ_AP012335:1580143-1580430 [Streptococcus pyogenes JRS4]
ATGACTACAGTAAAAAAAAACAGAGCGGTTACCTTTCAAGCTAATAAAGAATTGGTAAGCGAAGCAATGACAGTATTAAA
CAAAAAAAATTTAACCTTATCATCTGCTTTAAGATTATTCCTACAAAATGTCGTTGTCACAAACGAGGTTGACTTATTGA
CGGAAGAGGAGTTAGAGAAAGAAAAACTTTTCAAACAATTTCAAGCTGAAATCAACAAAAATATTGAGGATGTTCGTCAA
GGGAAGTTTTATACCTCTGAAGAAGTGAGGGCTGAACTTGGACTATAA

Structures

Toxin

• AlphaFold DB: A0A7Z2QKE7

Antitoxin

• AlphaFold DB: A0A4U7GX94