Detailed information of TA system TA030549

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/YafQ-DinJ
• Location: 1001789..1002339
• Replicon: chromosome
• Accession: NZ_AP012334
• Organism: Scardovia inopinata JCM 12537

Toxin (Protein)

• Gene name: relE
• Uniprot ID: W5IK21
• Locus tag: SCIP_RS04100
• Protein ID: WP_006293259.1
• Coordinates: 1001789..1002070 (-)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: W5IJR0
• Locus tag: SCIP_RS04105
• Protein ID: WP_006293260.1
• Coordinates: 1002067..1002339 (-)
• Length: 91 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
SCIP_RS08180	996951..998165	-	1215	WP_006293256.1	alpha/beta fold hydrolase	-
SCIP_RS04080	998219..999229	-	1011	WP_006293257.1	NADP-dependent oxidoreductase	-
SCIP_RS08185	999352..1000605	-	1254	WP_196792696.1	hypothetical protein	-
SCIP_RS07775	1000852..1001139	-	288	WP_171821033.1	hypothetical protein	-
SCIP_RS08050	1001180..1001284	-	105	WP_171821034.1	hypothetical protein	-
SCIP_RS08055	1001296..1001682	-	387	WP_040590642.1	hypothetical protein	-
SCIP_RS04100	1001789..1002070	-	282	WP_006293259.1	type II toxin-antitoxin system YafQ family toxin	Toxin
SCIP_RS04105	1002067..1002339	-	273	WP_006293260.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	Antitoxin
SCIP_RS08190	1002455..1002979	-	525	WP_006293261.1	hypothetical protein	-
SCIP_RS04115	1003344..1003940	-	597	WP_006293262.1	class I SAM-dependent methyltransferase	-
SCIP_RS04120	1003968..1004567	-	600	WP_006293263.1	alpha/beta hydrolase	-
SCIP_RS04125	1004613..1005746	-	1134	WP_006293264.1	glycosyl transferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, YafQ-like toxin (3-91)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (1-68)

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 11057.71 Da
• Isoelectric Point: 6.2273

>T30549 WP_006293259.1 NZ_AP012334:c1002070-1001789 [Scardovia inopinata JCM 12537]
MKYEIKYTNQFKKDLKLAKKQDKNLDKLFDAVNILAEGRTLEARYSDHDLSGNYKGTRECHIEPDWLLIYEIMDDILVLM
LYRLGSHSELFDK

Nucleotide

• Length: 282 bp

>T30549 NZ_AP012334:c1002070-1001789 [Scardovia inopinata JCM 12537]
ATGAAGTACGAAATAAAATATACAAACCAATTCAAAAAAGACCTGAAGCTTGCGAAGAAGCAGGACAAGAACCTGGATAA
ACTTTTTGACGCAGTGAATATCCTTGCCGAAGGCAGAACGCTGGAGGCAAGATATTCTGACCATGATTTGTCTGGAAATT
ATAAAGGTACCCGGGAATGCCATATAGAACCAGATTGGCTTTTAATTTATGAGATTATGGATGATATACTTGTTCTGATG
TTGTATCGTCTTGGATCGCATTCGGAATTATTTGACAAATGA

Antitoxin

Protein

• Length: 91 a.a.
• Molecular weight: 10126.52 Da
• Isoelectric Point: 4.4692

>AT30549 WP_006293260.1 NZ_AP012334:c1002339-1002067 [Scardovia inopinata JCM 12537]
MTTTNINIRMDKEIKDEAEQIFSELGLNMTTAVNIFFRTTIRENGIPFSLKLDIPNETTTAAIEEGKRIASDRNIKGYVS
MDDLKASLDV

Nucleotide

• Length: 273 bp

>AT30549 NZ_AP012334:c1002339-1002067 [Scardovia inopinata JCM 12537]
ATGACAACAACAAATATTAACATACGCATGGATAAGGAAATAAAAGACGAGGCAGAACAGATTTTTTCTGAACTAGGCTT
GAATATGACAACCGCTGTCAATATTTTTTTCAGGACCACGATTCGTGAGAATGGCATTCCCTTTTCATTGAAACTCGATA
TTCCGAATGAGACGACTACTGCGGCAATCGAAGAAGGCAAACGTATTGCTTCGGACCGAAATATCAAGGGGTATGTCAGC
ATGGATGATCTGAAAGCATCTCTTGACGTATGA

Structures

Toxin

• AlphaFold DB: W5IK21

Antitoxin

• AlphaFold DB: W5IJR0