Detailed information of TA system TA030548

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/YafQ-DinJ
• Location: 763110..763675
• Replicon: chromosome
• Accession: NZ_AP012334
• Organism: Scardovia inopinata JCM 12537

Toxin (Protein)

• Gene name: relE
• Uniprot ID: W5IJM5
• Locus tag: SCIP_RS03075
• Protein ID: WP_006293058.1
• Coordinates: 763385..763675 (+)
• Length: 97 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: W5IJK9
• Locus tag: SCIP_RS03070
• Protein ID: WP_040590572.1
• Coordinates: 763110..763388 (+)
• Length: 93 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
SCIP_RS03055	759775..760272	+	498	WP_081442808.1	type II 3-dehydroquinate dehydratase	-
SCIP_RS03060	760398..762080	+	1683	WP_006293054.1	CTP synthase	-
SCIP_RS03065	762270..762476	-	207	WP_006293055.1	transposon-encoded TnpW family protein	-
SCIP_RS07735	762595..762957	-	363	WP_115672902.1	cysteine-rich VLP domain-containing protein	-
SCIP_RS03070	763110..763388	+	279	WP_040590572.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	Antitoxin
SCIP_RS03075	763385..763675	+	291	WP_006293058.1	type II toxin-antitoxin system YafQ family toxin	Toxin
SCIP_RS03080	763712..764683	-	972	WP_196792690.1	MobA/MobL family protein	-
SCIP_RS08170	764989..765168	+	180	WP_196792704.1	hypothetical protein	-
SCIP_RS03085	765336..765587	-	252	WP_196792691.1	DUF3847 domain-containing protein	-
SCIP_RS03090	765584..766015	-	432	WP_006293061.1	sigma-70 family RNA polymerase sigma factor	-
SCIP_RS07740	766117..766533	-	417	WP_006293062.1	hypothetical protein	-
SCIP_RS03100	766922..767452	-	531	WP_196792692.1	hypothetical protein	-
SCIP_RS08175	767486..767686	-	201	WP_196792693.1	hypothetical protein	-
SCIP_RS03105	767683..768480	-	798	Protein_610	ABC transporter ATP-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	755685..773499	17814

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, YafQ-like toxin (5-94)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (7-70)

Sequences

Toxin

Protein

• Length: 97 a.a.
• Molecular weight: 11008.79 Da
• Isoelectric Point: 9.5079

>T30548 WP_006293058.1 NZ_AP012334:763385-763675 [Scardovia inopinata JCM 12537]
MRKTKYTVKFTTQFRKDYKLAIKRGLKIESLEEVVALLATGTPLPEKNKDHALSGDWAGHRDCHILPDWLLIYRIEDDVL
VLTLARTGTHSDLFGK

Nucleotide

• Length: 291 bp

>T30548 NZ_AP012334:763385-763675 [Scardovia inopinata JCM 12537]
ATGAGGAAAACAAAGTACACGGTCAAGTTCACCACGCAATTTCGTAAGGATTACAAGTTGGCGATAAAGCGCGGCCTGAA
AATCGAATCGCTGGAAGAGGTTGTTGCATTGCTGGCGACAGGTACGCCGCTGCCGGAGAAGAACAAAGACCATGCGCTTT
CCGGTGATTGGGCCGGACATCGGGACTGCCACATTCTCCCGGACTGGCTGTTGATTTACCGCATCGAGGATGATGTGCTG
GTGTTGACTTTGGCCCGAACCGGGACGCACAGCGACTTATTCGGAAAATAA

Antitoxin

Protein

• Length: 93 a.a.
• Molecular weight: 10162.58 Da
• Isoelectric Point: 4.4563

>AT30548 WP_040590572.1 NZ_AP012334:763110-763388 [Scardovia inopinata JCM 12537]
MAGNTTNISIRIDSDLKTQADALFGELGMNLTTAFNIFVRQSLREGGIPFDIKLDQPNKETIAAMLEAERIAKDPSVKGY
SDLDELFAELKK

Nucleotide

• Length: 279 bp

>AT30548 NZ_AP012334:763110-763388 [Scardovia inopinata JCM 12537]
ATGGCTGGGAATACAACGAATATCAGCATCCGCATAGATTCAGATTTGAAAACGCAGGCCGATGCCCTGTTCGGTGAGCT
TGGCATGAATTTGACCACTGCTTTTAATATTTTTGTTCGCCAATCGCTCCGGGAGGGCGGCATTCCCTTTGACATCAAAC
TGGATCAGCCCAACAAGGAAACGATAGCCGCCATGCTGGAGGCGGAAAGGATTGCGAAAGACCCGTCCGTAAAGGGCTAC
AGCGACTTGGATGAGCTGTTCGCGGAACTTAAAAAATGA

Structures

Toxin

• AlphaFold DB: W5IJM5

Antitoxin

• AlphaFold DB: W5IJK9