Detailed information of TA system TA030543

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PanAT/DUF4065(antitoxin)
• Location: 2081932..2082803
• Replicon: chromosome
• Accession: NZ_AP012327
• Organism: Bifidobacterium catenulatum subsp. kashiwanohense JCM 15439 = DSM 21854 strain JCM 15439

Toxin (Protein)

• Gene name: panT
• Uniprot ID: A0A087B0P1
• Locus tag: BBKW_RS08850
• Protein ID: WP_033501486.1
• Coordinates: 2082486..2082803 (+)
• Length: 106 a.a.

Antitoxin (Protein)

• Gene name: panA
• Uniprot ID: A0A087B0P0
• Locus tag: BBKW_RS08845
• Protein ID: WP_051920327.1
• Coordinates: 2081932..2082486 (+)
• Length: 185 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BBKW_RS08835	2078040..2078990	+	951	WP_033501484.1	glycosyltransferase family 2 protein	-
BBKW_RS08840	2079059..2081662	-	2604	WP_033501485.1	RICIN domain-containing protein	-
BBKW_RS08845	2081932..2082486	+	555	WP_051920327.1	DUF4065 domain-containing protein	Antitoxin
BBKW_RS08850	2082486..2082803	+	318	WP_033501486.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
BBKW_RS08855	2083081..2084007	+	927	WP_034255162.1	hypothetical protein	-
BBKW_RS08860	2084168..2085793	+	1626	WP_033501512.1	sialate O-acetylesterase	-
BBKW_RS08865	2085916..2086278	-	363	WP_033501487.1	hypothetical protein	-
BBKW_RS10625	2086653..2087156	-	504	WP_126016617.1	Na+:H+ dicarboxylate symporter	-
BBKW_RS08870	2087156..2087476	-	321	WP_033501488.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	2059824..2097707	37883

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (6-100)

Antitoxin

• Antitoxin SocA-like, Panacea domain (31-126)

Sequences

Toxin

Protein

• Length: 106 a.a.
• Molecular weight: 12637.75 Da
• Isoelectric Point: 9.3211

>T30543 WP_033501486.1 NZ_AP012327:2082486-2082803 [Bifidobacterium catenulatum subsp. kashiwanohense JCM 15439 = DSM]
MQPRPWQIWKVWFPFRENPSTGKYRPVVIKEVTEDNCVVIYVTSQVEKTHFPDFMLIHNWDEAGLTKASAIRYRKMLKIP
IDQLENYMGTLSPYDRIELQMKYLK

Nucleotide

• Length: 318 bp

>T30543 NZ_AP012327:2082486-2082803 [Bifidobacterium catenulatum subsp. kashiwanohense JCM 15439 = DSM]
ATGCAGCCGCGTCCTTGGCAAATTTGGAAAGTTTGGTTCCCATTCAGAGAAAACCCTTCCACAGGAAAATATCGGCCCGT
TGTTATCAAAGAAGTCACCGAAGATAATTGCGTAGTAATCTACGTTACGTCCCAAGTTGAAAAAACGCATTTTCCAGACT
TCATGCTTATCCACAATTGGGATGAAGCAGGTCTCACAAAGGCTTCAGCCATCCGCTATAGAAAGATGTTGAAAATCCCA
ATCGATCAGTTGGAAAACTACATGGGAACACTTTCGCCATACGATCGTATCGAACTCCAAATGAAATATCTGAAGTAA

Antitoxin

Protein

• Length: 185 a.a.
• Molecular weight: 21162.03 Da
• Isoelectric Point: 4.3019

>AT30543 WP_051920327.1 NZ_AP012327:2081932-2082486 [Bifidobacterium catenulatum subsp. kashiwanohense JCM 15439 = DSM]
MLNVMDVADFFIATANEGDPEEDDGMTNMKLNKLLFFAQAASLQRFGKPLFDAPLEAWKYGPVVKSVYRTFKECQRDSIA
KVAQPFDWQTIPPETLDLLCDVYRTYARDYSAMGLMRKTHQPDTPWSETYVPNENKVIPVESIQKWVQKQPLPIDMQPEP
DKNIIVAELDSQGRPFLPEGWEDD

Nucleotide

• Length: 555 bp

>AT30543 NZ_AP012327:2081932-2082486 [Bifidobacterium catenulatum subsp. kashiwanohense JCM 15439 = DSM]
ATGCTGAACGTTATGGACGTTGCCGATTTCTTCATCGCAACCGCCAACGAAGGCGATCCGGAAGAAGATGACGGCATGAC
CAATATGAAACTCAACAAGCTCCTGTTTTTTGCACAGGCAGCAAGCCTGCAACGATTCGGCAAACCCTTGTTCGACGCAC
CCCTCGAAGCTTGGAAATATGGCCCTGTCGTCAAAAGCGTATATCGGACGTTTAAGGAATGTCAGCGCGATTCCATCGCG
AAAGTCGCCCAACCGTTCGACTGGCAGACGATTCCACCGGAAACTTTGGATCTGCTGTGCGACGTATACCGCACATACGC
ACGGGATTATTCCGCAATGGGACTCATGCGCAAAACACACCAGCCTGACACCCCTTGGTCGGAAACTTATGTCCCTAATG
AGAACAAAGTCATCCCAGTTGAAAGCATCCAAAAATGGGTCCAAAAACAGCCTTTGCCTATCGACATGCAGCCCGAACCC
GATAAAAACATCATCGTTGCCGAGCTTGACTCGCAAGGGCGTCCGTTCCTCCCCGAGGGTTGGGAGGATGACTGA

Structures

Toxin

• AlphaFold DB: A0A087B0P1

Antitoxin

• AlphaFold DB: A0A087B0P0