Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mbcAT/RES-TIGR02293
Location 2204098..2204996 Replicon chromosome
Accession NZ_AM412059
Organism Mycobacterium tuberculosis variant bovis BCG str. Moreau RDJ isolate SL21 FAP RJ Passage B8S2 vaccine culture

Toxin (Protein)


Gene name mbcT Uniprot ID P64908
Locus tag H056_RS10255 Protein ID WP_003410001.1
Coordinates 2204098..2204658 (-) Length 187 a.a.

Antitoxin (Protein)


Gene name mbcA Uniprot ID P64910
Locus tag H056_RS10260 Protein ID WP_003410003.1
Coordinates 2204655..2204996 (-) Length 114 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H056_RS10225 2199267..2199920 - 654 WP_003409976.1 cutinase Cfp21 -
H056_RS10230 2200035..2200265 + 231 WP_003409980.1 DUF167 domain-containing protein -
H056_RS10235 2200350..2201261 - 912 WP_003409986.1 ArgP/LysG family DNA-binding transcriptional regulator -
H056_RS10240 2201370..2201969 + 600 WP_003409989.1 L-lysine exporter -
H056_RS10245 2202385..2202813 + 429 WP_003409992.1 cellulose-binding protein -
H056_RS10250 2203039..2203578 + 540 WP_003900446.1 23S rRNA (adenine(2058)-N(6))-methyltransferase Erm(37) -
H056_RS10255 2204098..2204658 - 561 WP_003410001.1 RES family NAD+ phosphorylase Toxin
H056_RS10260 2204655..2204996 - 342 WP_003410003.1 DUF2384 domain-containing protein Antitoxin
H056_RS10265 2205082..2205339 + 258 WP_003410006.1 hypothetical protein -
H056_RS10270 2205240..2205575 - 336 WP_003410009.1 dehydrogenase -
H056_RS10275 2205664..2206008 - 345 WP_003410010.1 type II toxin-antitoxin system toxin endoribonuclease MazF6 -
H056_RS10280 2206002..2206250 - 249 WP_003410014.1 ribbon-helix-helix protein, CopG family -
H056_RS10285 2206350..2208665 - 2316 WP_003899120.1 cation transporter ATPase CptG -
H056_RS10290 2208662..2208934 - 273 WP_003410017.1 DUF1490 family protein -
H056_RS10295 2208987..2209343 - 357 WP_003410018.1 Cd(II)/Pb(II)-sensing metalloregulatory transcriptional regulator CmtR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

RES domain

(25-148)

Antitoxin

Antitoxin Xre/MbcA/ParS-like, toxin-binding domain

(69-110)


Sequences

Toxin        


Download         Length: 187 a.a.        Molecular weight: 20248.70 Da        Isoelectric Point: 4.5091

>T30509 WP_003410001.1 NZ_AM412059:c2204658-2204098 [Mycobacterium tuberculosis variant bovis BCG str. Moreau RDJ]
VSDALDEGLVQRIDARGTIEWSETCYRYTGAHRDALSGEGARRFGGRWNPPLLFPAIYLADSAQACMVEVERAAQAASTT
AEKMLEAAYRLHTIDVTDLAVLDLTTPQAREAVGLENDDIYGDDWSGCQAVGHAAWFLHMQGVLVPAAGGVGLVVTAYEQ
RTRPGQLQLRQSVDLTPALYQELRAT

Download         Length: 561 bp

>T30509 NZ_AM412059:c2204658-2204098 [Mycobacterium tuberculosis variant bovis BCG str. Moreau RDJ]
GTGAGCGATGCCCTCGATGAAGGGCTCGTCCAGCGTATCGACGCACGCGGAACAATTGAGTGGTCGGAAACGTGCTACCG
GTATACCGGCGCGCACCGTGACGCCTTGTCCGGTGAGGGCGCGCGCAGATTCGGAGGCAGGTGGAATCCGCCGCTGCTCT
TTCCGGCGATCTATCTTGCTGATTCCGCCCAAGCCTGCATGGTTGAGGTGGAACGGGCGGCGCAAGCGGCTTCAACGACC
GCAGAGAAGATGCTCGAGGCGGCCTACCGACTACACACGATCGACGTCACGGACCTGGCCGTCCTCGATCTGACAACCCC
GCAAGCTCGGGAAGCCGTGGGGCTCGAGAACGACGACATCTATGGCGACGACTGGTCAGGGTGCCAGGCGGTCGGACATG
CGGCCTGGTTCTTGCACATGCAAGGTGTCCTCGTGCCGGCGGCGGGCGGTGTCGGCCTCGTTGTCACCGCGTATGAACAG
CGAACTCGGCCGGGCCAACTACAACTGCGACAAAGCGTCGATCTGACGCCTGCTCTTTACCAAGAACTTCGAGCCACGTA
G

Antitoxin


Download         Length: 114 a.a.        Molecular weight: 12488.33 Da        Isoelectric Point: 4.8359

>AT30509 WP_003410003.1 NZ_AM412059:c2204996-2204655 [Mycobacterium tuberculosis variant bovis BCG str. Moreau RDJ]
MGVNVLASTVSGAIERLGLTYEEVGDIVDASPRSVARWTAGQVVPQRLNKQRLIELAYVADALAEVLPRDQANVWMFSPN
RLLEHRKPADLVRDGEYQRVLALIDAMAEGVFV

Download         Length: 342 bp

>AT30509 NZ_AM412059:c2204996-2204655 [Mycobacterium tuberculosis variant bovis BCG str. Moreau RDJ]
ATGGGAGTAAATGTGCTCGCCTCGACCGTGTCGGGTGCGATCGAGCGCTTGGGATTGACCTACGAGGAAGTCGGTGACAT
CGTCGATGCCTCGCCGCGTTCCGTGGCGCGATGGACCGCAGGTCAGGTGGTTCCCCAACGCCTCAACAAGCAACGACTTA
TCGAGCTGGCCTATGTCGCCGACGCCCTCGCGGAAGTGCTGCCGCGTGACCAGGCGAACGTGTGGATGTTTTCGCCGAAT
CGGTTACTGGAACACCGCAAGCCTGCCGACCTCGTGCGAGACGGCGAGTACCAACGCGTGTTGGCGCTCATCGACGCGAT
GGCGGAGGGAGTGTTCGTGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6FKG

Antitoxin

Source ID Structure
PDB 6FKG
AlphaFold DB A0A7U4FAE4

References