Detailed information of TA system TA030488

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 756373..756913
• Replicon: chromosome
• Accession: NZ_AM412059
• Organism: Mycobacterium tuberculosis variant bovis BCG str. Moreau RDJ isolate SL21 FAP RJ Passage B8S2 vaccine culture

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: P9WII0
• Locus tag: H056_RS03455
• Protein ID: WP_003403376.1
• Coordinates: 756373..756681 (-)
• Length: 103 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: G0TQE0
• Locus tag: H056_RS03460
• Protein ID: WP_003403381.1
• Coordinates: 756668..756913 (-)
• Length: 82 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H056_RS03430	751688..753193	+	1506	WP_003403360.1	carotenoid cleavage oxygenase	-
H056_RS03435	753274..754284	+	1011	WP_003911248.1	ABC transporter ATP-binding protein	-
H056_RS03440	754672..755055	-	384	WP_003403365.1	ribonuclease VapC6	-
H056_RS03445	755150..755305	-	156	WP_003403368.1	type II toxin-antitoxin system VapB family antitoxin	-
H056_RS03450	755381..756097	-	717	WP_003403371.1	CPBP family intramembrane metalloprotease	-
H056_RS03455	756373..756681	-	309	WP_003403376.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
H056_RS03460	756668..756913	-	246	WP_003403381.1	ribbon-helix-helix protein, CopG family	Antitoxin
H056_RS03465	757023..757460	-	438	WP_003403386.1	type II toxin-antitoxin system VapC family toxin	-
H056_RS03470	757457..757711	-	255	WP_003911263.1	antitoxin VapB7	-
H056_RS03475	757825..760188	+	2364	WP_003403397.1	arylsulfatase AtsD	-
H056_RS03480	760251..760577	+	327	WP_003403401.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-
H056_RS03485	760489..760827	+	339	WP_003403405.1	PIN domain-containing protein	-
H056_RS03490	760824..760997	+	174	WP_003898549.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (1-101)

Antitoxin

• Ribbon-helix-helix protein, CopG (2-38)

Sequences

Toxin

Protein

• Length: 103 a.a.
• Molecular weight: 11320.10 Da
• Isoelectric Point: 11.6554

>T30488 WP_003403376.1 NZ_AM412059:c756681-756373 [Mycobacterium tuberculosis variant bovis BCG str. Moreau RDJ]
MRRGELWFAATPGGDRPVLVLTRDPVADRIGAVVVVALTRTRRGLVSELELTAVENRVPSDCVVNFDNIHTLPRTAFRRR
ITRLSPARLHEACQTLRASTGC

Nucleotide

• Length: 309 bp

>T30488 NZ_AM412059:c756681-756373 [Mycobacterium tuberculosis variant bovis BCG str. Moreau RDJ]
ATGCGGCGCGGTGAATTGTGGTTTGCCGCCACACCTGGTGGTGACAGACCAGTACTTGTCCTTACCAGAGATCCGGTGGC
AGACCGCATCGGCGCGGTCGTTGTGGTGGCCCTAACCCGCACCCGCCGAGGCCTGGTGTCGGAATTGGAGCTCACGGCCG
TCGAAAACCGTGTTCCGAGCGACTGCGTCGTCAACTTCGACAACATTCATACGTTGCCACGCACCGCATTCCGACGCCGC
ATCACCCGGCTGTCCCCGGCCCGCCTGCACGAAGCCTGTCAAACACTCCGGGCGAGCACGGGGTGTTGA

Antitoxin

Protein

• Length: 82 a.a.
• Molecular weight: 9169.01 Da
• Isoelectric Point: 4.2070

>AT30488 WP_003403381.1 NZ_AM412059:c756913-756668 [Mycobacterium tuberculosis variant bovis BCG str. Moreau RDJ]
MLSFRADDHDVDLADAWARRLHIGRSELLRDALRRHLAALAADQDVQAYTERPLTDDENALAEIADWGPAEDWADWADAA
R

Nucleotide

• Length: 246 bp

>AT30488 NZ_AM412059:c756913-756668 [Mycobacterium tuberculosis variant bovis BCG str. Moreau RDJ]
ATGCTCAGCTTCCGCGCCGACGACCACGACGTCGACCTGGCCGACGCGTGGGCGCGTCGCCTACACATCGGCCGCTCCGA
GCTGCTGCGCGATGCGCTGCGACGTCACTTGGCCGCGCTGGCCGCGGACCAGGATGTCCAGGCCTACACCGAGCGACCGT
TGACGGACGACGAGAACGCACTGGCTGAGATCGCCGACTGGGGGCCGGCGGAGGACTGGGCCGACTGGGCCGATGCGGCG
CGGTGA

Structures

Toxin

• AlphaFold DB: A0A7U4BSE5

Antitoxin

• AlphaFold DB: G0TQE0