Detailed information of TA system TA030462

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/-
• Location: 128004..128563
• Replicon: plasmid pRSB225
• Accession: NC_025100
• Organism: uncultured bacterium

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: pRSB225_174
• Protein ID: YP_009066129.1
• Coordinates: 128288..128563 (+)
• Length: 92 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: pRSB225_173
• Protein ID: YP_009066128.1
• Coordinates: 128004..128288 (+)
• Length: 95 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
pRSB225_167	123467..123694	-	228	YP_009066122.1	oxidoreductase	-
pRSB225_168	123725..123907	-	183	YP_009066123.1	oxidoreductase	-
pRSB225_169	124308..125198	+	891	YP_009066124.1	dihydrodipicolinate synthase	-
pRSB225_170	125223..125603	+	381	YP_009066125.1	translation initiation inhibitor protein	-
pRSB225_171	125636..126601	+	966	YP_009066126.1	2-keto-3-deoxygluconate permease	-
pRSB225_172	126647..127399	-	753	YP_009066127.1	HTH-type transcriptional regulator YfaX	-
pRSB225_173	128004..128288	+	285	YP_009066128.1	transcriptional regulator	Antitoxin
pRSB225_174	128288..128563	+	276	YP_009066129.1	plasmid stabilization system protein	Toxin
pRSB225_175	128669..128962	+	294	YP_009066130.1	hypothetical protein	-
pRSB225_176	129158..130327	-	1170	YP_009066131.1	hypothetical protein	-
pRSB225_177	130859..131041	+	183	YP_009066132.1	hypothetical protein	-
pRSB225_178	130997..131293	-	297	YP_009066133.1	hypothetical protein	-
pRSB225_179	131773..132513	+	741	YP_009066134.1	integrase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	aph(3')-Ia / aph(6)-Id / aph(3'')-Ib / sul2 / blaTEM-1B / mph(A) / dfrA14 / catA1 / tet(B) / sitABCD	iutA / iucD / iucC / iucB / iucA	1..164550	164550

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (3-90)

Antitoxin

• Ribbon-helix-helix protein, CopG (14-51)

Sequences

Toxin

Protein

• Length: 92 a.a.
• Molecular weight: 10749.45 Da
• Isoelectric Point: 10.0871

>T30462 YP_009066129.1 NC_025100:128288-128563 [uncultured bacterium]
MELKWTSKALSDLSRLFDFLVLASKPAAARTVQSLTQAPVILLTHPRMGEQLFQFEPREVRRIFAGEYEIRYEINGQTIY
VLRLWHTRENR

Nucleotide

• Length: 276 bp

>T30462 NC_025100:128288-128563 [uncultured bacterium]
ATGGAACTGAAGTGGACCAGTAAGGCGCTTTCTGATTTGTCGCGGTTATTTGATTTTCTGGTGCTGGCCAGTAAACCTGC
TGCCGCCAGAACGGTACAGTCCCTGACACAGGCACCGGTCATTCTGTTAACTCATCCACGTATGGGAGAACAGTTGTTTC
AGTTTGAACCCAGGGAGGTCAGACGGATTTTTGCTGGCGAGTACGAAATCCGTTACGAAATTAATGGCCAGACTATTTAT
GTATTGCGTCTGTGGCACACACGAGAAAACAGGTAG

Antitoxin

Protein

• Length: 95 a.a.
• Molecular weight: 10460.87 Da
• Isoelectric Point: 5.6558

>AT30462 YP_009066128.1 NC_025100:128004-128288 [uncultured bacterium]
MQMKNNAAQATKVITAHVPLPMADKVDQMAARLERSRGWVIKQALSAWLAQEEERNRLTLEALDDVTSGQVIDHQAVQAW
SDSLSTDNPLPVPR

Nucleotide

• Length: 285 bp

>AT30462 NC_025100:128004-128288 [uncultured bacterium]
ATGCAAATGAAAAACAATGCCGCACAAGCAACAAAAGTAATTACCGCGCATGTGCCATTACCTATGGCTGATAAAGTCGA
CCAGATGGCCGCCAGACTGGAACGTTCCCGGGGCTGGGTTATCAAACAGGCGCTTTCTGCATGGCTTGCCCAGGAGGAGG
AGCGTAATCGCCTGACGCTGGAAGCCCTGGACGATGTGACATCCGGACAGGTTATCGACCATCAGGCTGTACAGGCCTGG
TCGGACAGCCTCAGTACTGACAATCCGTTACCGGTGCCACGCTGA