Detailed information of TA system TA030461

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pemIK/-
• Location: 66974..67565
• Replicon: plasmid pRSB225
• Accession: NC_025100
• Organism: uncultured bacterium

Toxin (Protein)

• Gene name: pemK
• Uniprot ID: -
• Locus tag: pRSB225_93
• Protein ID: YP_009066050.1
• Coordinates: 67233..67565 (+)
• Length: 111 a.a.

Antitoxin (Protein)

• Gene name: pemI
• Uniprot ID: -
• Locus tag: pRSB225_92
• Protein ID: YP_009066049.1
• Coordinates: 66974..67231 (+)
• Length: 86 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
pRSB225_86	62954..63379	+	426	YP_009066043.1	transposase	-
pRSB225_87	63376..63726	+	351	YP_009066044.1	transposase	-
pRSB225_88	63757..65370	+	1614	YP_009066045.1	transposase	-
pRSB225_89	65748..66038	+	291	YP_009066046.1	replication initiation protein RepA4	-
pRSB225_90	65990..66130	+	141	YP_009066047.1	hypothetical protein	-
pRSB225_91	66228..66881	+	654	YP_009066048.1	transfer inhibition protein	-
pRSB225_92	66974..67231	+	258	YP_009066049.1	stable plasmid inheritance protein PemI	Antitoxin
pRSB225_93	67233..67565	+	333	YP_009066050.1	programmed cell death toxin PemK	Toxin
pRSB225_94	67998..68273	+	276	YP_009066051.1	transposase InsA	-
pRSB225_95	68192..68695	+	504	YP_009066052.1	Insertion element IS1 protein insB	-
pRSB225_96	68802..69236	-	435	YP_009066053.1	mercuric resistance operon regulatory protein	-
pRSB225_97	69308..69658	+	351	YP_009066054.1	mercuric ion transport protein MerT	-
pRSB225_98	69672..69947	+	276	YP_009066055.1	periplasmic mercuric ion binding transport protein MerP	-
pRSB225_99	69983..70405	+	423	YP_009066056.1	inner membrane mercuric uptake protein MerC	-
pRSB225_100	70457..72151	+	1695	YP_009066057.1	mercuric ion reductase MerA	-
pRSB225_101	72169..72531	+	363	YP_009066058.1	mercuric resistance transcriptional repressor protein MerD	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	aph(3')-Ia / aph(6)-Id / aph(3'')-Ib / sul2 / blaTEM-1B / mph(A) / dfrA14 / catA1 / tet(B) / sitABCD	iutA / iucD / iucC / iucB / iucA	1..164550	164550
	-	inside	IScluster/Tn	-	-	62954..68695	5741

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (2-108)

Antitoxin

• Antitoxin PemI-like (4-84)

Sequences

Toxin

Protein

• Length: 111 a.a.
• Molecular weight: 11880.78 Da
• Isoelectric Point: 11.1995

>T30461 YP_009066050.1 NC_025100:67233-67565 [uncultured bacterium]
MERGEIWLVSLDPTAGHEQQGTRPVLIVTPAAFNRVTRLPVVVPVTSGGNFARTAGFAVSLDGVGIRTTGVVRCDQPRTI
DMKARGGKRLERVPETIMNEVLGRLSTILT

Nucleotide

• Length: 333 bp

>T30461 NC_025100:67233-67565 [uncultured bacterium]
ATGGAAAGAGGGGAAATCTGGCTTGTCTCGCTTGATCCTACCGCAGGTCATGAGCAGCAGGGAACGCGGCCGGTGCTGAT
TGTCACACCGGCGGCCTTTAATCGCGTGACCCGCCTGCCTGTTGTTGTGCCCGTAACCAGCGGAGGCAATTTTGCCCGCA
CTGCCGGCTTTGCGGTGTCGTTGGATGGTGTTGGCATACGTACCACAGGTGTTGTACGTTGCGATCAACCCCGGACAATT
GATATGAAAGCACGGGGCGGAAAACGACTCGAACGGGTTCCGGAGACTATCATGAACGAAGTTCTTGGCCGCCTGTCCAC
TATTCTGACTTGA

Antitoxin

Protein

• Length: 86 a.a.
• Molecular weight: 9332.61 Da
• Isoelectric Point: 4.2694

>AT30461 YP_009066049.1 NC_025100:66974-67231 [uncultured bacterium]
MHTTRLKRVGGSVMLTVPPALLNALSLGTDNEVGMVIDNGRLIVEPYRRPQYSLAELLAQCDPNAEISAEEREWLDAPAT
GQEEI

Nucleotide

• Length: 258 bp

>AT30461 NC_025100:66974-67231 [uncultured bacterium]
ATGCATACCACCCGACTGAAGAGGGTTGGCGGCTCAGTTATGCTGACCGTCCCACCGGCACTGCTGAATGCGCTGTCTCT
GGGCACAGATAATGAAGTTGGCATGGTCATTGATAATGGCCGGCTGATTGTTGAGCCGTACAGACGCCCGCAATATTCAC
TGGCTGAGCTACTGGCACAGTGTGATCCGAATGCTGAAATATCAGCTGAAGAACGAGAATGGCTGGATGCACCGGCGACT
GGTCAGGAGGAAATCTGA