Detailed information of TA system TA030441

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PumAB/COG3657-dnstrm_HI1420
• Location: 4750717..4751303
• Replicon: chromosome
• Accession: NC_023149
• Organism: Pseudomonas aeruginosa SCV20265

Toxin (Protein)

• Gene name: PumA
• Uniprot ID: G8CP73
• Locus tag: SCV20265_RS22115
• Protein ID: WP_003120987.1
• Coordinates: 4751004..4751303 (-)
• Length: 100 a.a.

Antitoxin (Protein)

• Gene name: PumB
• Uniprot ID: -
• Locus tag: SCV20265_RS22110
• Protein ID: WP_003448662.1
• Coordinates: 4750717..4751007 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
SCV20265_RS22090	4745868..4746077	+	210	WP_003105733.1	cold-shock protein	-
SCV20265_RS22095	4746299..4748188	+	1890	WP_016851610.1	hypothetical protein	-
SCV20265_RS22100	4748185..4750161	+	1977	WP_016851611.1	DEAD/DEAH box helicase	-
SCV20265_RS22105	4750302..4750646	+	345	WP_016851612.1	hypothetical protein	-
SCV20265_RS22110	4750717..4751007	-	291	WP_003448662.1	putative addiction module antidote protein	Antitoxin
SCV20265_RS22115	4751004..4751303	-	300	WP_003120987.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
SCV20265_RS22120	4751505..4752629	+	1125	WP_012076859.1	TcpQ domain-containing protein	-
SCV20265_RS22125	4752629..4754338	+	1710	WP_012076860.1	PilN family type IVB pilus formation outer membrane protein	-
SCV20265_RS22130	4754342..4755667	+	1326	WP_003099758.1	type 4b pilus protein PilO2	-
SCV20265_RS22135	4755657..4756190	+	534	WP_003099760.1	type IV pilus biogenesis protein PilP	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	4723738..4816893	93155

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (3-96)

Antitoxin

• Cro/C1-type helix-turn-helix domain (47-90)

Sequences

Toxin

Protein

• Length: 100 a.a.
• Molecular weight: 11133.88 Da
• Isoelectric Point: 10.4495

>T30441 WP_003120987.1 NC_023149:c4751303-4751004 [Pseudomonas aeruginosa SCV20265]
MVEVKQTATFMAWESKLKDRRAKAVIAARIFRLANGLPGDVSPVGQGVSELRIHYGPGYRVYFQQRGTEIVILLCGGDKS
SQARDIEMAKRLANEWRPQ

Nucleotide

• Length: 300 bp

>T30441 NC_023149:c4751303-4751004 [Pseudomonas aeruginosa SCV20265]
ATGGTCGAAGTCAAACAAACCGCCACTTTCATGGCATGGGAAAGCAAGCTAAAGGATCGGCGGGCAAAGGCCGTGATCGC
CGCCCGTATCTTCCGATTGGCGAATGGACTACCCGGCGATGTGTCACCAGTCGGCCAGGGTGTGAGCGAGTTGAGGATTC
ACTATGGCCCAGGCTATCGGGTGTACTTCCAGCAGCGCGGGACGGAAATCGTCATCCTGCTATGTGGTGGAGACAAGAGC
AGCCAGGCCAGGGATATAGAAATGGCGAAACGCCTGGCAAATGAGTGGAGGCCGCAATGA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10394.91 Da
• Isoelectric Point: 4.7527

>AT30441 WP_003448662.1 NC_023149:c4751007-4750717 [Pseudomonas aeruginosa SCV20265]
MTEQIYDYDPAQALDSPEAMALFIADALDTGDTAYIAKAMGVVARAKGMTELAKETGLAREQLYKSFSERGNPTLKTMLA
VMRALGVDLTARPHAQ

Nucleotide

• Length: 291 bp

>AT30441 NC_023149:c4751007-4750717 [Pseudomonas aeruginosa SCV20265]
ATGACCGAGCAAATTTATGATTATGACCCCGCGCAAGCGCTGGACAGCCCGGAGGCCATGGCTCTGTTCATCGCAGACGC
TTTGGATACTGGAGACACCGCCTATATCGCTAAGGCTATGGGAGTCGTGGCCCGCGCCAAGGGGATGACGGAGTTGGCCA
AGGAAACGGGTTTGGCCAGGGAGCAGCTTTACAAATCGTTTAGTGAGCGCGGGAACCCTACATTGAAAACCATGCTGGCA
GTAATGCGAGCTCTGGGAGTTGATCTGACCGCCCGCCCGCATGCTCAGTGA

Structures

Toxin

• AlphaFold DB: G8CP73