Detailed information of TA system TA030438

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PP_4151-4152/excise(antitoxin)
• Location: 2460706..2461748
• Replicon: chromosome
• Accession: NC_023149
• Organism: Pseudomonas aeruginosa SCV20265

Toxin (Protein)

• Gene name: PP_4152
• Uniprot ID: -
• Locus tag: SCV20265_RS11635
• Protein ID: WP_003109777.1
• Coordinates: 2461173..2461748 (+)
• Length: 192 a.a.

Antitoxin (Protein)

• Gene name: PP_4151
• Uniprot ID: I3TV68
• Locus tag: SCV20265_RS11630
• Protein ID: WP_003050245.1
• Coordinates: 2460706..2461176 (+)
• Length: 157 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
SCV20265_RS11595	2456098..2457516	-	1419	WP_020924934.1	TIGR03752 family integrating conjugative element protein	-
SCV20265_RS11600	2457506..2458417	-	912	WP_003105643.1	TIGR03749 family integrating conjugative element protein	-
SCV20265_RS11605	2458414..2459106	-	693	WP_003105641.1	TIGR03746 family integrating conjugative element protein	-
SCV20265_RS11610	2459103..2459501	-	399	WP_003105639.1	TIGR03750 family conjugal transfer protein	-
SCV20265_RS11615	2459513..2459872	-	360	WP_003090173.1	TIGR03745 family integrating conjugative element membrane protein	-
SCV20265_RS11620	2459889..2460122	-	234	WP_003050225.1	TIGR03758 family integrating conjugative element protein	-
SCV20265_RS11625	2460119..2460502	-	384	WP_003120001.1	RAQPRD family integrative conjugative element protein	-
SCV20265_RS11630	2460706..2461176	+	471	WP_003050245.1	helix-turn-helix domain-containing protein	Antitoxin
SCV20265_RS11635	2461173..2461748	+	576	WP_003109777.1	PIN domain-containing protein	Toxin
SCV20265_RS11640	2461766..2462680	+	915	WP_003105629.1	AAA family ATPase	-
SCV20265_RS11645	2462677..2463147	+	471	WP_003105626.1	hypothetical protein	-
SCV20265_RS11650	2463144..2463644	+	501	WP_003090159.1	hypothetical protein	-
SCV20265_RS11655	2463644..2464546	+	903	WP_003105624.1	nucleotidyltransferase domain-containing protein	-
SCV20265_RS11660	2464585..2465310	+	726	WP_003050273.1	CBASS effector endonuclease NucC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	2412167..2511282	99115

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (9-114)

Antitoxin

• Helix-turn-helix domain, group 17 (85-133)

Sequences

Toxin

Protein

• Length: 192 a.a.
• Molecular weight: 21644.79 Da
• Isoelectric Point: 5.6172

>T30438 WP_003109777.1 NC_023149:2461173-2461748 [Pseudomonas aeruginosa SCV20265]
MRHSPFTAVYDACVLYPAPLRDFLMWLGLSGRFRARWSQAIHEEWKRNLLINRPDLTRVQVDRTSDLMDRAIPDGLVEGY
EALVAGLTLPDPDDRHVLAAAIRCGASVIVTFNERDFPNDLLAPYGIESQHPDEFVDNLLDLDAAAVVSAAQRQRAQLKH
PPIEVDRYLEILLRQGLVQTTKVLATYRTIL

Nucleotide

• Length: 576 bp

>T30438 NC_023149:2461173-2461748 [Pseudomonas aeruginosa SCV20265]
ATGAGGCATTCCCCATTCACCGCCGTCTACGACGCGTGCGTTCTATATCCCGCGCCACTGCGGGATTTCCTGATGTGGCT
CGGCCTGTCTGGCCGCTTCCGGGCGCGGTGGAGCCAAGCCATTCATGAGGAGTGGAAACGCAACCTGTTGATCAACCGCC
CCGATCTCACCCGGGTTCAGGTCGACAGGACGTCGGATCTCATGGACCGGGCAATTCCGGACGGCTTGGTGGAGGGCTAC
GAAGCGCTCGTGGCAGGCCTGACATTGCCCGATCCGGACGACCGGCACGTCCTGGCTGCGGCGATTCGCTGCGGTGCGAG
CGTGATTGTGACGTTCAACGAACGGGATTTCCCGAACGATCTGCTGGCTCCGTACGGCATCGAATCGCAGCACCCCGATG
AGTTTGTGGACAACCTACTGGATCTGGACGCGGCCGCCGTGGTGTCGGCTGCGCAGCGCCAGCGTGCCCAACTCAAGCAT
CCGCCGATCGAGGTGGACCGCTATCTCGAAATCCTGCTGCGCCAGGGCCTTGTGCAAACGACCAAGGTGCTGGCGACCTA
TCGCACCATTCTCTGA

Antitoxin

Protein

• Length: 157 a.a.
• Molecular weight: 17245.62 Da
• Isoelectric Point: 6.3803

>AT30438 WP_003050245.1 NC_023149:2460706-2461176 [Pseudomonas aeruginosa SCV20265]
MTTATAQSKMTLPAAGEVKAAVQGQRALAAYLATQFETQHIQIFDDHKQAHQVELPTSALRLLVDILAELADGNAVKVVP
VHAELTTQEAADLLNVSRPHFVKLLEDGVLAFHRTGKHRRVRFADLMQYKEARERASEQAMAELAQQSQELGMGYE

Nucleotide

• Length: 471 bp

>AT30438 NC_023149:2460706-2461176 [Pseudomonas aeruginosa SCV20265]
ATGACCACCGCCACTGCCCAATCCAAGATGACCCTTCCCGCCGCGGGAGAGGTGAAGGCAGCCGTCCAGGGTCAACGTGC
CTTGGCGGCTTACCTCGCAACCCAGTTCGAGACGCAGCACATCCAGATCTTTGATGACCACAAACAGGCTCATCAGGTGG
AACTGCCTACCTCGGCTTTACGCCTGCTGGTCGACATCCTGGCCGAGTTGGCCGATGGCAATGCAGTAAAGGTGGTGCCC
GTCCATGCAGAGCTGACGACCCAAGAGGCGGCGGACTTGCTCAACGTGTCCCGTCCCCATTTCGTCAAGCTGCTCGAAGA
TGGGGTGTTGGCATTTCATCGCACCGGCAAGCACCGCAGGGTGAGGTTCGCCGATCTGATGCAGTACAAGGAAGCGCGCG
AGCGCGCCAGTGAGCAGGCGATGGCCGAACTCGCTCAGCAGTCGCAAGAGTTGGGAATGGGATACGAATGA

Structures

Antitoxin

• AlphaFold DB: I3TV68