Detailed information of TA system TA030432

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-couple_hipB
• Location: 1077039..1078593
• Replicon: chromosome
• Accession: NC_023137
• Organism: Phaeobacter gallaeciensis DSM 26640

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: V9WF80
• Locus tag: GAL_RS05155
• Protein ID: WP_024096523.1
• Coordinates: 1077292..1078593 (+)
• Length: 434 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: -
• Locus tag: GAL_RS05150
• Protein ID: WP_024096522.1
• Coordinates: 1077039..1077290 (+)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GAL_RS05140	1074688..1075470	+	783	WP_024096519.1	hypothetical protein	-
GAL_RS05145	1076035..1076898	+	864	WP_024096521.1	hypothetical protein	-
GAL_RS05150	1077039..1077290	+	252	WP_024096522.1	helix-turn-helix domain-containing protein	Antitoxin
GAL_RS05155	1077292..1078593	+	1302	WP_024096523.1	type II toxin-antitoxin system HipA family toxin	Toxin
GAL_RS05160	1078864..1079061	+	198	WP_024096524.1	hypothetical protein	-
GAL_RS05165	1079329..1080231	-	903	WP_024096525.1	LysR family transcriptional regulator	-
GAL_RS21890	1080328..1080933	+	606	WP_024096526.1	putative protein-disulfide isomerase	-
GAL_RS05175	1080951..1081814	+	864	WP_024096527.1	MBL fold metallo-hydrolase	-
GAL_RS05180	1082076..1082465	+	390	WP_040103922.1	nuclear transport factor 2 family protein	-
GAL_RS05185	1082547..1082948	+	402	Protein_1008	DoxX family protein	-
GAL_RS05190	1082964..1083587	+	624	WP_024096530.1	protocatechuate 3,4-dioxygenase beta subunit	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	katA	1075737..1177498	101761

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA, N-terminal subdomain 1 (10-109)

Antitoxin

• Cro/C1-type helix-turn-helix domain (12-67)

Sequences

Toxin

Protein

• Length: 434 a.a.
• Molecular weight: 48048.86 Da
• Isoelectric Point: 5.5137

>T30432 WP_024096523.1 NC_023137:1077292-1078593 [Phaeobacter gallaeciensis DSM 26640]
MGRKRSYTPLNVFLNSRLVGQLVREPSGGVSFAYTRDWLEWEHRMPVSLSLPLQENRYIGAPVMAVFDNLLPDSDLIRRK
VAERVGAEGVDAFSLLSQIGRDCIGALQFLPDGQEPQPMSELTGEGVNEAQIGAILSDLDLAPLGIRRENDFRISVAGAQ
EKTALLRKDGQWIEPTGTTPTTHIIKPQIGRLPNGMDLSDSVENEYLCLKLMEAFGLRVANAEMAQFGEKKALVVERFDR
RWTSVGRLIRLPQEDCCQALSVVPTQKYQNEGGPGISDIMDLLLGSDDPNKDRYDFFKANVLFWLLGATDGHGKNFSVSL
LPGGRFRMTPLYDVLTVQPTVDARQIERKYFKLAMNFGNSNHYKVSNIVGRHIVETGVQSGLSRAVVQGLFEELQETSHA
IVEATFNQLPADLPESLLASIDGALKARLHLLN

Nucleotide

• Length: 1302 bp

>T30432 NC_023137:1077292-1078593 [Phaeobacter gallaeciensis DSM 26640]
ATGGGCCGCAAGCGCAGCTACACACCGCTGAACGTTTTCCTGAACTCGCGGCTCGTGGGCCAATTGGTACGTGAGCCCTC
GGGCGGCGTCAGTTTCGCCTATACGCGAGACTGGCTCGAATGGGAGCACCGGATGCCTGTGTCGCTATCATTGCCATTGC
AGGAAAACCGCTACATCGGCGCACCTGTGATGGCCGTCTTTGACAACCTGTTGCCCGACAGTGACCTCATTCGACGAAAA
GTCGCAGAGCGCGTCGGGGCCGAGGGCGTCGATGCCTTTAGCTTGCTGTCCCAAATTGGGCGCGACTGCATTGGTGCGCT
GCAGTTCCTTCCCGACGGGCAAGAGCCACAGCCAATGTCCGAGCTGACTGGAGAAGGTGTGAATGAAGCTCAGATTGGGG
CAATTCTGAGCGACCTCGATCTCGCGCCACTGGGTATTCGCCGCGAAAATGACTTTCGCATTTCTGTCGCGGGCGCGCAG
GAAAAAACAGCGCTGCTTCGTAAAGATGGCCAGTGGATTGAGCCAACCGGTACGACGCCAACCACGCACATCATCAAGCC
GCAAATCGGCAGGCTGCCCAATGGCATGGATCTGTCTGACAGTGTAGAAAACGAATACCTTTGCCTCAAGTTGATGGAGG
CGTTTGGGCTTCGGGTTGCGAATGCCGAGATGGCGCAATTCGGCGAAAAGAAAGCCTTGGTCGTTGAGCGTTTTGATCGG
CGCTGGACGTCAGTTGGCCGCTTGATCCGTCTGCCGCAGGAGGATTGCTGTCAAGCATTGTCCGTTGTGCCTACCCAGAA
GTACCAGAACGAGGGCGGCCCAGGAATCTCAGATATTATGGATCTATTGTTGGGAAGTGATGACCCCAACAAAGACCGGT
ACGATTTTTTCAAAGCGAATGTCCTGTTCTGGTTGCTGGGCGCGACAGACGGACATGGTAAAAACTTCAGCGTCAGCCTC
TTACCCGGCGGTCGCTTTCGCATGACACCGCTTTACGACGTTTTGACGGTGCAGCCGACTGTCGATGCGCGACAGATCGA
GCGAAAGTACTTCAAACTGGCCATGAATTTCGGCAACTCAAACCACTACAAGGTTTCGAATATCGTTGGTCGGCACATCG
TGGAGACTGGAGTTCAATCCGGCTTATCCCGCGCTGTCGTACAGGGCCTATTCGAAGAACTGCAGGAGACCTCGCACGCG
ATCGTTGAGGCCACGTTCAACCAGCTTCCAGCGGATCTCCCAGAGTCGTTGCTTGCTTCGATCGATGGCGCGCTGAAGGC
CAGGTTGCACCTGTTGAACTGA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9128.43 Da
• Isoelectric Point: 8.7279

>AT30432 WP_024096522.1 NC_023137:1077039-1077290 [Phaeobacter gallaeciensis DSM 26640]
MKHLVRTSKNLGHAIRQARKAKNLTQKELASMSGVWQETISKIENGSGGTKLETVFDLIAALELEITVTERSKGTSDGLE
DIF

Nucleotide

• Length: 252 bp

>AT30432 NC_023137:1077039-1077290 [Phaeobacter gallaeciensis DSM 26640]
ATGAAGCACCTCGTTCGTACATCAAAAAACTTGGGCCACGCAATTCGCCAGGCTCGGAAAGCAAAGAACCTTACCCAGAA
GGAACTCGCCTCGATGAGCGGTGTCTGGCAGGAGACAATTTCCAAGATCGAGAACGGCAGTGGGGGAACCAAGCTGGAAA
CGGTATTCGATTTGATCGCGGCCCTGGAGCTCGAAATTACGGTGACCGAGCGCAGCAAGGGCACATCAGACGGGCTTGAG
GACATTTTCTGA

Structures

Toxin

• AlphaFold DB: V9WF80