Detailed information of TA system TA030431

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): parDE/ParE-CC2985
• Location: 59600..60131
• Replicon: plasmid unnamed
• Accession: NC_023136
• Organism: Leisingera methylohalidivorans DSM 14336

Toxin (Protein)

• Gene name: parE
• Uniprot ID: V9W094
• Locus tag: METH_RS21440
• Protein ID: WP_024092391.1
• Coordinates: 59600..59902 (-)
• Length: 101 a.a.

Antitoxin (Protein)

• Gene name: parD
• Uniprot ID: V9W0R4
• Locus tag: METH_RS21445
• Protein ID: WP_024092392.1
• Coordinates: 59895..60131 (-)
• Length: 79 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
METH_RS21420	56004..56687	+	684	WP_024092387.1	hypothetical protein	-
METH_RS21425	56793..58067	+	1275	WP_024092388.1	Vi polysaccharide biosynthesis UDP-N-acetylglucosamine C-6 dehydrogenase TviB	-
METH_RS21430	58133..58567	+	435	WP_024092389.1	GFA family protein	-
METH_RS21435	58627..59538	-	912	WP_024092390.1	MurR/RpiR family transcriptional regulator	-
METH_RS21440	59600..59902	-	303	WP_024092391.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
METH_RS21445	59895..60131	-	237	WP_024092392.1	type II toxin-antitoxin system ParD family antitoxin	Antitoxin
METH_RS21450	60289..64353	-	4065	WP_044008825.1	YidC/Oxa1 family membrane protein insertase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	clpV1 / hsiC1/vipB / hsiB1/vipA	1..285395	285395

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (7-93)

Antitoxin

• Antitoxin ParD (3-70)

Sequences

Toxin

Protein

• Length: 101 a.a.
• Molecular weight: 11477.20 Da
• Isoelectric Point: 6.7260

>T30431 WP_024092391.1 NC_023136:c59902-59600 [Leisingera methylohalidivorans DSM 14336]
MAEPRWRLRPAARADLAAIWRYGLETWGETQADAYADSLFALFDLIAGFPGMARERTEFTPPVRIHPTGAHLVIYRLEQG
RPEIIRILHARQDLMAFLSE

Nucleotide

• Length: 303 bp

>T30431 NC_023136:c59902-59600 [Leisingera methylohalidivorans DSM 14336]
ATGGCTGAACCGCGCTGGCGCCTGCGCCCCGCCGCCCGTGCCGACCTCGCCGCCATCTGGCGCTACGGGCTGGAAACCTG
GGGTGAAACACAGGCCGATGCCTATGCCGACAGCCTGTTTGCCCTGTTCGATCTGATCGCCGGCTTCCCCGGAATGGCCC
GCGAACGGACTGAATTCACCCCGCCGGTGCGCATTCACCCGACCGGCGCGCATCTGGTGATCTACCGGCTGGAGCAGGGA
CGCCCCGAGATCATCCGCATCCTGCACGCCCGCCAGGACCTGATGGCCTTCCTTTCGGAATAG

Antitoxin

Protein

• Length: 79 a.a.
• Molecular weight: 8372.34 Da
• Isoelectric Point: 4.6544

>AT30431 WP_024092392.1 NC_023136:c60131-59895 [Leisingera methylohalidivorans DSM 14336]
MATMNVSLPDQMKDWVEDQARAGTYANSSDYVRDLIRRDQVRAAAIGELQSAIDAGLASGPAEALTAEGFKAAMRRNG

Nucleotide

• Length: 237 bp

>AT30431 NC_023136:c60131-59895 [Leisingera methylohalidivorans DSM 14336]
ATGGCCACCATGAACGTATCCTTGCCGGATCAGATGAAAGACTGGGTCGAAGACCAGGCCCGCGCCGGCACCTATGCCAA
TTCCAGCGACTATGTCCGCGACCTGATCCGCCGCGACCAGGTCCGCGCGGCCGCCATTGGCGAACTGCAATCCGCCATCG
ACGCAGGCCTTGCCAGCGGCCCGGCAGAGGCGCTGACAGCCGAAGGGTTCAAGGCCGCTATGCGCCGCAATGGCTGA

Structures

Toxin

• AlphaFold DB: V9W094

Antitoxin

• AlphaFold DB: V9W0R4