Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | spoIISABC/SpoIISA-SpoIISB |
| Location | 1275923..1276840 | Replicon | chromosome |
| Accession | NC_023073 | ||
| Organism | Bacillus amyloliquefaciens LFB112 | ||
Toxin (Protein)
| Gene name | spoIISA | Uniprot ID | A0A6A8LGT8 |
| Locus tag | U722_RS06410 | Protein ID | WP_003154806.1 |
| Coordinates | 1276094..1276840 (-) | Length | 249 a.a. |
Antitoxin (Protein)
| Gene name | spoIISB | Uniprot ID | I2HQ14 |
| Locus tag | U722_RS06405 | Protein ID | WP_003154807.1 |
| Coordinates | 1275923..1276093 (-) | Length | 57 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| U722_RS06370 | 1271155..1272777 | + | 1623 | WP_024085192.1 | pyocin knob domain-containing protein | - |
| U722_RS06375 | 1272790..1273161 | + | 372 | WP_024085193.1 | hypothetical protein | - |
| U722_RS06380 | 1273167..1273364 | + | 198 | WP_003154819.1 | XkdX family protein | - |
| U722_RS06385 | 1273421..1274182 | + | 762 | WP_024085194.1 | hypothetical protein | - |
| U722_RS06390 | 1274234..1274497 | + | 264 | WP_003154815.1 | hemolysin XhlA family protein | - |
| U722_RS06395 | 1274511..1274774 | + | 264 | WP_003154813.1 | phage holin | - |
| U722_RS06400 | 1274788..1275666 | + | 879 | WP_024085195.1 | N-acetylmuramoyl-L-alanine amidase | - |
| U722_RS06405 | 1275923..1276093 | - | 171 | WP_003154807.1 | type II toxin-antitoxin system SpoIISB family antitoxin | Antitoxin |
| U722_RS06410 | 1276094..1276840 | - | 747 | WP_003154806.1 | type II toxin-antitoxin system SpoIISA family toxin | Toxin |
| U722_RS06415 | 1276945..1277943 | - | 999 | WP_003154805.1 | inorganic phosphate transporter | - |
| U722_RS06420 | 1277956..1278573 | - | 618 | WP_003154804.1 | DUF47 domain-containing protein | - |
| U722_RS06425 | 1278859..1280175 | - | 1317 | WP_003154801.1 | amino acid permease | - |
| U722_RS06430 | 1280499..1281449 | + | 951 | WP_024085196.1 | ring-cleaving dioxygenase | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 249 a.a. Molecular weight: 29034.50 Da Isoelectric Point: 4.6947
>T30427 WP_003154806.1 NC_023073:c1276840-1276094 [Bacillus amyloliquefaciens LFB112]
MLLFFQIMVWTMAAALILYVYASWRYEAKVKEKMFAIRKTWYLLFVVGAMVYWTYDPESLFAAWRQYLIVAVCFALIDAF
IFLSAYIKKLAGNELETDTREILEENNEMLHSYLEKLKTYQYLLKNEPIHVYYGSTEAYAEGISRLLAAYGEKMNVTASL
CDYSAQSDKDRLTEHMPDAADVQSRLNRKDVYYDQKGRLVLIPFTVQDRHYVIKLTSENLLTEFDYLLFTSLTSIYDLML
PIEEEGDG
MLLFFQIMVWTMAAALILYVYASWRYEAKVKEKMFAIRKTWYLLFVVGAMVYWTYDPESLFAAWRQYLIVAVCFALIDAF
IFLSAYIKKLAGNELETDTREILEENNEMLHSYLEKLKTYQYLLKNEPIHVYYGSTEAYAEGISRLLAAYGEKMNVTASL
CDYSAQSDKDRLTEHMPDAADVQSRLNRKDVYYDQKGRLVLIPFTVQDRHYVIKLTSENLLTEFDYLLFTSLTSIYDLML
PIEEEGDG
Download Length: 747 bp
>T30427 NC_023073:c1276840-1276094 [Bacillus amyloliquefaciens LFB112]
ATGCTTTTGTTTTTTCAGATCATGGTGTGGACGATGGCGGCCGCACTCATTTTATATGTGTACGCATCCTGGCGGTATGA
AGCAAAGGTAAAAGAAAAAATGTTTGCCATCCGTAAAACGTGGTATTTACTATTTGTAGTAGGCGCCATGGTCTATTGGA
CATATGATCCCGAATCGCTCTTTGCCGCATGGAGACAATATTTGATTGTTGCCGTCTGCTTTGCCCTTATTGATGCGTTT
ATCTTTTTGAGCGCGTATATCAAAAAGCTCGCCGGCAATGAATTAGAGACGGATACGCGGGAAATTTTAGAAGAAAATAA
CGAAATGCTTCACTCCTATCTTGAAAAACTGAAAACCTATCAATATCTGTTGAAAAATGAACCGATCCATGTATATTATG
GAAGTACAGAAGCTTATGCGGAAGGCATCAGCAGACTGCTGGCCGCTTATGGAGAAAAAATGAATGTCACGGCTTCCTTG
TGCGACTATTCCGCACAATCGGATAAGGACCGCTTGACGGAGCATATGCCTGATGCGGCGGACGTTCAGTCCCGTCTTAA
CCGTAAAGACGTCTACTATGATCAAAAAGGCAGGCTTGTGCTTATTCCGTTTACAGTCCAGGACCGCCATTACGTGATCA
AACTCACGTCGGAAAACCTGTTGACGGAATTCGATTATTTGCTTTTCACATCGCTAACGAGCATTTATGATTTAATGCTG
CCAATAGAAGAGGAAGGTGACGGTTAA
ATGCTTTTGTTTTTTCAGATCATGGTGTGGACGATGGCGGCCGCACTCATTTTATATGTGTACGCATCCTGGCGGTATGA
AGCAAAGGTAAAAGAAAAAATGTTTGCCATCCGTAAAACGTGGTATTTACTATTTGTAGTAGGCGCCATGGTCTATTGGA
CATATGATCCCGAATCGCTCTTTGCCGCATGGAGACAATATTTGATTGTTGCCGTCTGCTTTGCCCTTATTGATGCGTTT
ATCTTTTTGAGCGCGTATATCAAAAAGCTCGCCGGCAATGAATTAGAGACGGATACGCGGGAAATTTTAGAAGAAAATAA
CGAAATGCTTCACTCCTATCTTGAAAAACTGAAAACCTATCAATATCTGTTGAAAAATGAACCGATCCATGTATATTATG
GAAGTACAGAAGCTTATGCGGAAGGCATCAGCAGACTGCTGGCCGCTTATGGAGAAAAAATGAATGTCACGGCTTCCTTG
TGCGACTATTCCGCACAATCGGATAAGGACCGCTTGACGGAGCATATGCCTGATGCGGCGGACGTTCAGTCCCGTCTTAA
CCGTAAAGACGTCTACTATGATCAAAAAGGCAGGCTTGTGCTTATTCCGTTTACAGTCCAGGACCGCCATTACGTGATCA
AACTCACGTCGGAAAACCTGTTGACGGAATTCGATTATTTGCTTTTCACATCGCTAACGAGCATTTATGATTTAATGCTG
CCAATAGAAGAGGAAGGTGACGGTTAA
Antitoxin
Download Length: 57 a.a. Molecular weight: 6676.74 Da Isoelectric Point: 10.9273
>AT30427 WP_003154807.1 NC_023073:c1276093-1275923 [Bacillus amyloliquefaciens LFB112]
MEQAFQNGQPEQRAKPFKMFKKRSMTSIASYQVSPHTARIFKENERLIDKYKQKKA
MEQAFQNGQPEQRAKPFKMFKKRSMTSIASYQVSPHTARIFKENERLIDKYKQKKA
Download Length: 171 bp
>AT30427 NC_023073:c1276093-1275923 [Bacillus amyloliquefaciens LFB112]
ATGGAGCAGGCATTTCAAAACGGGCAGCCGGAACAAAGAGCGAAACCGTTCAAAATGTTTAAAAAACGTTCAATGACAAG
TATCGCAAGTTACCAGGTCAGCCCTCATACCGCCAGAATTTTCAAAGAAAACGAGCGGCTGATTGACAAGTATAAACAAA
AAAAAGCTTGA
ATGGAGCAGGCATTTCAAAACGGGCAGCCGGAACAAAGAGCGAAACCGTTCAAAATGTTTAAAAAACGTTCAATGACAAG
TATCGCAAGTTACCAGGTCAGCCCTCATACCGCCAGAATTTTCAAAGAAAACGAGCGGCTGATTGACAAGTATAAACAAA
AAAAAGCTTGA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
Structures
Toxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A6A8LGT8 |
Antitoxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | I2HQ14 |