Detailed information of TA system TA030426

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 507723..508360
• Replicon: chromosome
• Accession: NC_023073
• Organism: Bacillus amyloliquefaciens LFB112

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: G4NU33
• Locus tag: U722_RS02450
• Protein ID: WP_003156187.1
• Coordinates: 508010..508360 (+)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: I2HMS5
• Locus tag: U722_RS02445
• Protein ID: WP_003156188.1
• Coordinates: 507723..508004 (+)
• Length: 94 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
U722_RS02425	504088..504687	-	600	WP_003156193.1	rhomboid family intramembrane serine protease	-
U722_RS02430	504780..505145	+	366	WP_003156192.1	holo-ACP synthase	-
U722_RS02435	505310..506317	+	1008	WP_003156191.1	outer membrane lipoprotein carrier protein LolA	-
U722_RS02440	506434..507603	+	1170	WP_003156189.1	alanine racemase	-
U722_RS02445	507723..508004	+	282	WP_003156188.1	type II toxin-antitoxin system antitoxin EndoAI	Antitoxin
U722_RS02450	508010..508360	+	351	WP_003156187.1	type II toxin-antitoxin system endoribonuclease NdoA	Toxin
U722_RS02455	508478..509299	+	822	WP_014304404.1	STAS domain-containing protein	-
U722_RS02460	509304..509669	+	366	WP_003156180.1	RsbT antagonist protein RsbS	-
U722_RS02465	509672..510073	+	402	WP_003156178.1	anti-sigma regulatory factor	-
U722_RS02470	510085..511092	+	1008	WP_003156177.1	PP2C family protein-serine/threonine phosphatase	-
U722_RS02475	511156..511485	+	330	WP_003156176.1	anti-sigma factor antagonist RsbV	-
U722_RS02480	511482..511964	+	483	WP_003156173.1	anti-sigma B factor RsbW	-
U722_RS02485	511930..512718	+	789	WP_003156171.1	RNA polymerase sigma factor SigB	-
U722_RS02490	512718..513320	+	603	WP_003156170.1	SpoIIE family protein phosphatase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (4-111)

Antitoxin

• Ribbon-helix-helix protein, CopG (12-45)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12977.98 Da
• Isoelectric Point: 4.8781

>T30426 WP_003156187.1 NC_023073:508010-508360 [Bacillus amyloliquefaciens LFB112]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Nucleotide

• Length: 351 bp

>T30426 NC_023073:508010-508360 [Bacillus amyloliquefaciens LFB112]
TTGATTGTAAAGCGCGGCGATGTTTATTTTGCTGATTTATCTCCTGTTGTTGGCTCAGAGCAAGGCGGCGTACGCCCGGT
TTTAGTGATCCAGAATGATATCGGAAATCGCTTTAGCCCGACTGCTATTGTTGCAGCCATAACAGCCCAAATACAGAAAG
CGAAATTACCAACCCACGTCGAAATTGATGCAAAACGCTACGGTTTTGAAAGAGATTCCGTTATTTTGCTGGAGCAAATT
CGGACGATTGACAAGCAAAGATTAACGGATAAAATAACTCATCTGGATGATGAAATGATGGATAAGGTTGATGAAGCCCT
GCAAATCAGTTTGGCACTCATTGATTTTTAG

Antitoxin

Protein

• Length: 94 a.a.
• Molecular weight: 10562.92 Da
• Isoelectric Point: 6.4706

>AT30426 WP_003156188.1 NC_023073:507723-508004 [Bacillus amyloliquefaciens LFB112]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHYAEC
EAETTVERLVSGG

Nucleotide

• Length: 282 bp

>AT30426 NC_023073:507723-508004 [Bacillus amyloliquefaciens LFB112]
TTGTCTGAATCCAGCGCAAGAACCGAAATGAAAATCAGCTTGCCCGAAAACTTAGTAGCTGAATTGGATGGTGTAGCGAT
GCGGGAGAAACGAAGCAGAAACGAACTGATATCACAAGCAGTGAGAGCGTATGTCAGCGAACGGACAACTCGCCATAACC
GTGATTTGATGAGACGCGGGTATATGGAAATGGCGAAAATCAACTTGAATATCTCTTCTGAGGCTCACTATGCTGAGTGC
GAAGCAGAAACGACCGTTGAGCGCTTAGTCAGCGGAGGATAA

Structures

Toxin

• PDB: 4MDX
• PDB: 4ME7
• PDB: 1NE8

Antitoxin

• AlphaFold DB: I2HMS5