Detailed information of TA system TA030422

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PP_4151-4152/excise(antitoxin)
• Location: 2815039..2816081
• Replicon: chromosome
• Accession: NC_023066
• Organism: Pseudomonas aeruginosa LES431

Toxin (Protein)

• Gene name: PP_4152
• Uniprot ID: -
• Locus tag: T223_RS13495
• Protein ID: WP_003153636.1
• Coordinates: 2815506..2816081 (+)
• Length: 192 a.a.

Antitoxin (Protein)

• Gene name: PP_4151
• Uniprot ID: I3TV68
• Locus tag: T223_RS13490
• Protein ID: WP_003050245.1
• Coordinates: 2815039..2815509 (+)
• Length: 157 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
T223_RS13455	2810418..2811836	-	1419	WP_004350605.1	TIGR03752 family integrating conjugative element protein	-
T223_RS13460	2811826..2812737	-	912	WP_004350604.1	TIGR03749 family integrating conjugative element protein	-
T223_RS13465	2812734..2813426	-	693	WP_012614071.1	TIGR03746 family integrating conjugative element protein	-
T223_RS13470	2813423..2813833	-	411	WP_003821108.1	TIGR03750 family conjugal transfer protein	-
T223_RS13475	2813846..2814205	-	360	WP_003153634.1	TIGR03745 family integrating conjugative element membrane protein	-
T223_RS13480	2814222..2814455	-	234	WP_003050225.1	TIGR03758 family integrating conjugative element protein	-
T223_RS13485	2814452..2814835	-	384	WP_003090167.1	RAQPRD family integrative conjugative element protein	-
T223_RS13490	2815039..2815509	+	471	WP_003050245.1	helix-turn-helix domain-containing protein	Antitoxin
T223_RS13495	2815506..2816081	+	576	WP_003153636.1	PIN domain-containing protein	Toxin
T223_RS13500	2816099..2817013	+	915	WP_003050256.1	AAA family ATPase	-
T223_RS13505	2817010..2817480	+	471	WP_003153638.1	hypothetical protein	-
T223_RS13510	2817477..2817977	+	501	WP_003090159.1	hypothetical protein	-
T223_RS13515	2817977..2818879	+	903	WP_003153640.1	nucleotidyltransferase domain-containing protein	-
T223_RS13520	2818918..2819643	+	726	WP_003050273.1	CBASS effector endonuclease NucC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	2754491..2865608	111117

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (9-114)

Antitoxin

• Helix-turn-helix domain, group 17 (85-133)

Sequences

Toxin

Protein

• Length: 192 a.a.
• Molecular weight: 21629.78 Da
• Isoelectric Point: 5.9995

>T30422 WP_003153636.1 NC_023066:2815506-2816081 [Pseudomonas aeruginosa LES431]
MRHSPFTAVYDACVLYPAPLRDFLMWLGLSGRFRARWSQAIHEEWKRNLLINRPDLTRVQVDRTSDLMDRAIPDGLVEGY
EALVAGLTLPDPNDRHVLAAAIRCGASVIVTFNERDFPNDLLAPYGIESQHPDEFVDNLLDLDAAAVVSAAQRQRAQLKH
PPIDVDRYLEILLRQGLVQTTKVLATYRTIL

Nucleotide

• Length: 576 bp

>T30422 NC_023066:2815506-2816081 [Pseudomonas aeruginosa LES431]
ATGAGGCATTCCCCATTCACCGCCGTCTACGACGCGTGCGTTCTATATCCCGCGCCACTGCGGGATTTCCTGATGTGGCT
CGGCCTGTCTGGCCGCTTCCGGGCGCGGTGGAGCCAAGCCATTCATGAGGAGTGGAAACGCAATCTGTTGATCAACCGCC
CCGATCTCACCCGGGTTCAGGTCGACAGGACGTCGGATCTCATGGACAGGGCCATTCCGGACGGCTTGGTGGAGGGCTAC
GAAGCGCTCGTGGCAGGCCTGACATTACCCGATCCGAACGATCGGCACGTCCTGGCTGCGGCGATTCGCTGCGGTGCGAG
CGTGATTGTGACGTTCAACGAACGTGATTTCCCGAACGATCTGCTGGCTCCGTACGGCATCGAATCGCAGCACCCCGATG
AGTTTGTGGACAACCTGCTGGATCTGGATGCGGCCGCCGTAGTGTCGGCTGCGCAGCGCCAGCGTGCCCAACTCAAGCAT
CCGCCGATCGATGTGGACCGCTATCTCGAAATCCTGCTGCGCCAAGGCCTTGTGCAAACGACCAAGGTGCTGGCGACCTA
TCGCACCATTCTCTGA

Antitoxin

Protein

• Length: 157 a.a.
• Molecular weight: 17245.62 Da
• Isoelectric Point: 6.3803

>AT30422 WP_003050245.1 NC_023066:2815039-2815509 [Pseudomonas aeruginosa LES431]
MTTATAQSKMTLPAAGEVKAAVQGQRALAAYLATQFETQHIQIFDDHKQAHQVELPTSALRLLVDILAELADGNAVKVVP
VHAELTTQEAADLLNVSRPHFVKLLEDGVLAFHRTGKHRRVRFADLMQYKEARERASEQAMAELAQQSQELGMGYE

Nucleotide

• Length: 471 bp

>AT30422 NC_023066:2815039-2815509 [Pseudomonas aeruginosa LES431]
ATGACCACCGCCACTGCCCAATCCAAGATGACCCTTCCCGCCGCGGGAGAGGTTAAGGCAGCCGTCCAGGGTCAACGTGC
CTTGGCGGCTTACCTCGCAACCCAGTTCGAGACGCAGCACATCCAGATCTTTGATGACCACAAACAGGCTCATCAGGTGG
AACTGCCTACCTCGGCTTTACGCCTGCTGGTCGACATCCTGGCCGAGTTGGCCGATGGCAATGCAGTAAAGGTGGTGCCC
GTCCATGCAGAGCTGACGACCCAAGAGGCGGCGGACTTGCTCAACGTGTCCCGTCCCCATTTCGTCAAGCTGCTAGAAGA
TGGGGTGTTGGCATTTCATCGCACCGGCAAGCACCGCAGGGTGAGGTTCGCCGATCTGATGCAGTACAAGGAAGCGCGCG
AGCGCGCCAGTGAGCAGGCGATGGCCGAACTCGCTCAGCAGTCGCAAGAGTTGGGAATGGGATACGAATGA

Structures

Antitoxin

• AlphaFold DB: I3TV68