Detailed information of TA system TA030418

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): shpA-brnA/BrnT_toxin-BrnA
• Location: 3406960..3407539
• Replicon: chromosome
• Accession: NC_023065
• Organism: Magnetospirillum gryphiswaldense MSR-1 v2

Toxin (Protein)

• Gene name: shpA
• Uniprot ID: A4U0V7
• Locus tag: MGMSRV2_RS16405
• Protein ID: WP_041634718.1
• Coordinates: 3407273..3407539 (-)
• Length: 89 a.a.

Antitoxin (Protein)

• Gene name: brnA
• Uniprot ID: -
• Locus tag: MGMSRV2_RS16400
• Protein ID: WP_024081481.1
• Coordinates: 3406960..3407286 (-)
• Length: 109 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MGMSRV2_RS16375	3402660..3403016	+	357	WP_024081474.1	hypothetical protein	-
MGMSRV2_RS16380	3403039..3403512	+	474	WP_024081475.1	YchJ family protein	-
MGMSRV2_RS16385	3403521..3404465	+	945	WP_024081476.1	glycosyltransferase family 2 protein	-
MGMSRV2_RS16390	3404604..3404924	-	321	WP_144084388.1	hypothetical protein	-
MGMSRV2_RS16395	3405073..3406677	+	1605	WP_024081478.1	CHASE domain-containing protein	-
MGMSRV2_RS16400	3406960..3407286	-	327	WP_024081481.1	BrnA antitoxin family protein	Antitoxin
MGMSRV2_RS16405	3407273..3407539	-	267	WP_041634718.1	BrnT family toxin	Toxin
MGMSRV2_RS16410	3407680..3407934	+	255	WP_041633694.1	hypothetical protein	-
MGMSRV2_RS16415	3407959..3408318	+	360	WP_024081483.1	hemerythrin domain-containing protein	-
MGMSRV2_RS16420	3408346..3409248	+	903	Protein_3208	IS1595 family transposase	-
MGMSRV2_RS16425	3409598..3409861	-	264	WP_024081485.1	hypothetical protein	-
MGMSRV2_RS16430	3409918..3410367	-	450	WP_024081486.1	hypothetical protein	-
MGMSRV2_RS16435	3410485..3410703	+	219	WP_041633696.1	hypothetical protein	-
MGMSRV2_RS21200	3410875..3411246	-	372	WP_024081487.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	3408334..3409434	1100

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (5-81)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (28-104)

Sequences

Toxin

Protein

• Length: 89 a.a.
• Molecular weight: 10060.48 Da
• Isoelectric Point: 7.2028

>T30418 WP_041634718.1 NC_023065:c3407539-3407273 [Magnetospirillum gryphiswaldense MSR-1 v2]
MPIEFDAVKNAINVAKHGVDFKVVDAFEWDTAVVVEDTRREYGETRLIALGYIGDRLHVLAFTIRDGNIRPISLRKANSR
EIRRYAEA

Nucleotide

• Length: 267 bp

>T30418 NC_023065:c3407539-3407273 [Magnetospirillum gryphiswaldense MSR-1 v2]
ATGCCTATAGAATTCGACGCCGTTAAAAATGCCATCAACGTCGCCAAGCATGGCGTCGATTTTAAGGTGGTCGACGCATT
TGAATGGGACACAGCTGTGGTGGTCGAAGATACCCGCCGGGAATATGGCGAAACACGTCTAATCGCCCTTGGGTATATCG
GTGACCGCTTGCACGTACTGGCTTTTACCATCCGTGACGGGAACATTCGTCCCATCTCTTTGCGTAAAGCCAATTCCAGG
GAGATTAGGCGTTATGCCGAAGCTTAA

Antitoxin

Protein

• Length: 109 a.a.
• Molecular weight: 11692.27 Da
• Isoelectric Point: 6.3867

>AT30418 WP_024081481.1 NC_023065:c3407286-3406960 [Magnetospirillum gryphiswaldense MSR-1 v2]
MPKLKPSTIIPTDEEDAAISAAISTDPDSPELTDANFANARPASEVLANYLGATETEALLRRRGRPLKADRKVDIKLRID
PDVVEALRSGGKGWQTRANEILRKGVGL

Nucleotide

• Length: 327 bp

>AT30418 NC_023065:c3407286-3406960 [Magnetospirillum gryphiswaldense MSR-1 v2]
ATGCCGAAGCTTAAGCCATCGACCATCATACCGACCGACGAGGAAGATGCTGCAATTTCCGCTGCAATCAGCACCGATCC
AGATTCGCCAGAGTTGACCGATGCCAATTTTGCCAATGCCCGTCCTGCTTCCGAGGTTCTGGCAAATTATCTTGGTGCCA
CCGAGACTGAGGCGCTTTTGCGTCGTCGTGGTCGTCCGTTGAAAGCCGACCGCAAGGTGGACATCAAGCTGCGCATTGAT
CCTGATGTTGTTGAGGCTCTGCGGTCTGGTGGAAAGGGTTGGCAGACCCGTGCCAATGAGATTTTGCGTAAAGGCGTTGG
GCTGTAG

Structures

Toxin

• AlphaFold DB: A4U0V7