Detailed information of TA system TA030417

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ccdAB/CcdA(antitoxin)
• Location: 3000226..3000767
• Replicon: chromosome
• Accession: NC_023065
• Organism: Magnetospirillum gryphiswaldense MSR-1 v2

Toxin (Protein)

• Gene name: ccdB
• Uniprot ID: -
• Locus tag: MGMSRV2_RS14295
• Protein ID: WP_024081066.1
• Coordinates: 3000456..3000767 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: ccdA
• Uniprot ID: -
• Locus tag: MGMSRV2_RS14290
• Protein ID: WP_024081065.1
• Coordinates: 3000226..3000456 (+)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MGMSRV2_RS14270	2995227..2995859	-	633	WP_041634556.1	endonuclease III	-
MGMSRV2_RS14275	2995893..2996804	-	912	WP_024081062.1	DUF1848 domain-containing protein	-
MGMSRV2_RS14280	2996950..2998857	+	1908	WP_024081063.1	molecular chaperone DnaK	-
MGMSRV2_RS14285	2999017..3000165	+	1149	WP_024081064.1	molecular chaperone DnaJ	-
MGMSRV2_RS14290	3000226..3000456	+	231	WP_024081065.1	type II toxin-antitoxin system CcdA family antitoxin	Antitoxin
MGMSRV2_RS14295	3000456..3000767	+	312	WP_024081066.1	CcdB family protein	Toxin
MGMSRV2_RS14300	3000785..3001582	+	798	WP_024081067.1	4-hydroxy-tetrahydrodipicolinate reductase	-
MGMSRV2_RS14305	3001552..3002745	-	1194	WP_024081068.1	PQQ-dependent sugar dehydrogenase	-
MGMSRV2_RS14310	3002795..3003385	-	591	WP_024081069.1	nucleotide exchange factor GrpE	-
MGMSRV2_RS14315	3003406..3004458	-	1053	WP_024081070.1	heat-inducible transcriptional repressor HrcA	-
MGMSRV2_RS14320	3004592..3005317	+	726	WP_024081071.1	ribonuclease PH	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin CcdB (3-102)

Antitoxin

• Post-segregation antitoxin CcdA (6-76)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 11052.89 Da
• Isoelectric Point: 4.2309

>T30417 WP_024081066.1 NC_023065:3000456-3000767 [Magnetospirillum gryphiswaldense MSR-1 v2]
MAQFDVYANPVAGMEGGAPFVVDVQSDHLAGLPTRMIMPLAALDDCQPLRHLNPPMEVGDERFALMTQFMAAVPRQVLGT
PVGSLADQRALILAALDFLFTGI

Nucleotide

• Length: 312 bp

>T30417 NC_023065:3000456-3000767 [Magnetospirillum gryphiswaldense MSR-1 v2]
ATGGCTCAGTTCGACGTTTATGCCAATCCGGTCGCAGGGATGGAAGGTGGCGCCCCTTTTGTCGTCGATGTGCAATCCGA
CCATCTGGCCGGCTTGCCCACACGCATGATCATGCCCTTGGCCGCCCTCGATGATTGCCAGCCCCTTCGTCACCTTAACC
CGCCGATGGAGGTAGGTGACGAACGCTTTGCCTTGATGACCCAATTCATGGCAGCCGTCCCCCGTCAGGTGCTGGGCACC
CCGGTGGGCAGTCTGGCCGACCAGCGCGCCCTTATCCTCGCCGCCCTGGATTTCCTGTTCACCGGCATCTGA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8797.03 Da
• Isoelectric Point: 8.0531

>AT30417 WP_024081065.1 NC_023065:3000226-3000456 [Magnetospirillum gryphiswaldense MSR-1 v2]
MNAVPKKSCNLSINAELLREAKGLGINLSQTLEVQLEKLVREARAKAWAEENRDAIESQNRWIEKHGLFSDHFRFF

Nucleotide

• Length: 231 bp

>AT30417 NC_023065:3000226-3000456 [Magnetospirillum gryphiswaldense MSR-1 v2]
ATGAACGCAGTGCCGAAAAAGTCCTGCAATCTCAGCATCAATGCCGAGCTACTGCGCGAGGCCAAGGGCTTGGGCATCAA
TCTGTCCCAGACCCTGGAAGTGCAGTTGGAAAAGCTGGTGCGCGAGGCCCGCGCCAAGGCCTGGGCCGAGGAAAACCGCG
ACGCCATCGAAAGCCAGAACCGCTGGATCGAGAAACACGGTTTGTTTTCTGATCATTTCCGGTTCTTCTGA