Detailed information of TA system TA030412

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/LOTUS_5_Limkain_b1-Phd
• Location: 717063..717730
• Replicon: chromosome
• Accession: NC_023065
• Organism: Magnetospirillum gryphiswaldense MSR-1 v2

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: MGMSRV2_RS03395
• Protein ID: WP_024078932.1
• Coordinates: 717326..717730 (+)
• Length: 135 a.a.

Antitoxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: MGMSRV2_RS03390
• Protein ID: WP_084028218.1
• Coordinates: 717063..717326 (+)
• Length: 88 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MGMSRV2_RS03350	712383..712811	-	429	WP_024078923.1	hypothetical protein	-
MGMSRV2_RS03355	712862..713119	+	258	WP_024078924.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	-
MGMSRV2_RS03360	713116..713388	+	273	WP_024078925.1	type II toxin-antitoxin system YafQ family toxin	-
MGMSRV2_RS03365	713395..714033	-	639	WP_024078926.1	hypothetical protein	-
MGMSRV2_RS03370	714030..714326	-	297	WP_024078927.1	hypothetical protein	-
MGMSRV2_RS03375	714326..715348	-	1023	WP_024078928.1	major capsid protein	-
MGMSRV2_RS03380	715362..715751	-	390	WP_024078929.1	head decoration protein	-
MGMSRV2_RS03385	715764..716990	-	1227	WP_024078930.1	S49 family peptidase	-
MGMSRV2_RS03390	717063..717326	+	264	WP_084028218.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
MGMSRV2_RS03395	717326..717730	+	405	WP_024078932.1	type II toxin-antitoxin system VapC family toxin	Toxin
MGMSRV2_RS03400	717711..719144	-	1434	WP_024078933.1	phage portal protein	-
MGMSRV2_RS03405	719147..719359	-	213	WP_024078934.1	hypothetical protein	-
MGMSRV2_RS03410	719365..721248	-	1884	WP_197538567.1	phage terminase large subunit family protein	-
MGMSRV2_RS03415	721292..721837	-	546	WP_024078936.1	hypothetical protein	-
MGMSRV2_RS03420	721936..722292	+	357	WP_024078937.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	675809..726787	50978

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (3-120)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (7-53)

Sequences

Toxin

Protein

• Length: 135 a.a.
• Molecular weight: 15053.23 Da
• Isoelectric Point: 5.2314

>T30412 WP_024078932.1 NC_023065:717326-717730 [Magnetospirillum gryphiswaldense MSR-1 v2]
MFLLDTVVLSELRKRDRSPNVVRWLTDKAADDIFLSSVTIGEIERGIVRQRVKDPAFAEALESWLDRTIQIYGDRILPVD
TQIARRWGSLSARIGNDGADLLIAATALEHGLTVVTRNIRHFEPTGVALIDPFE

Nucleotide

• Length: 405 bp

>T30412 NC_023065:717326-717730 [Magnetospirillum gryphiswaldense MSR-1 v2]
ATGTTTCTGCTCGACACCGTGGTCCTGTCCGAACTGCGCAAGCGGGACCGGAGCCCCAACGTCGTTCGCTGGCTGACCGA
CAAGGCGGCGGATGACATCTTCCTGAGTTCGGTGACCATCGGCGAGATCGAGCGGGGCATTGTCCGCCAGAGGGTCAAGG
ATCCTGCCTTCGCCGAAGCCCTAGAATCATGGCTCGATCGCACCATCCAGATTTACGGTGACCGCATCCTGCCGGTGGAT
ACCCAGATCGCCCGCCGGTGGGGCAGTTTGAGCGCCCGTATCGGCAACGATGGGGCCGATCTGCTGATCGCCGCCACCGC
CCTGGAACATGGGCTGACCGTGGTCACCCGCAACATCCGGCATTTCGAACCTACTGGCGTCGCGCTTATTGATCCGTTTG
AATAG

Antitoxin

Protein

• Length: 88 a.a.
• Molecular weight: 9890.24 Da
• Isoelectric Point: 6.4819

>AT30412 WP_084028218.1 NC_023065:717063-717326 [Magnetospirillum gryphiswaldense MSR-1 v2]
VMPEPRWSLQDAKNSFSAVVDAALHGRPQTVTKRGKPAVVVLSVQEYERLHQRHDVGTPSFVEHLLAMPQGEVEFERQPV
TLREVEF

Nucleotide

• Length: 264 bp

>AT30412 NC_023065:717063-717326 [Magnetospirillum gryphiswaldense MSR-1 v2]
GTGATGCCGGAACCGCGTTGGTCCCTTCAAGATGCCAAGAACAGCTTCAGCGCCGTGGTCGACGCGGCCCTGCATGGGCG
TCCGCAAACCGTCACCAAGCGAGGCAAACCAGCCGTCGTCGTTTTGTCGGTTCAGGAATACGAGCGACTGCATCAGCGAC
ACGACGTCGGCACACCGTCGTTCGTCGAGCACCTTCTGGCCATGCCGCAAGGTGAAGTCGAATTTGAACGCCAGCCGGTC
ACGTTGCGTGAGGTCGAGTTCTGA