Detailed information of TA system TA030398

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PemK-MazE
• Location: 3131789..3132324
• Replicon: chromosome
• Accession: NC_023036
• Organism: Mycobacterium neoaurum VKM Ac-1815D

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: V5XEF3
• Locus tag: D174_RS14610
• Protein ID: WP_023985812.1
• Coordinates: 3131992..3132324 (+)
• Length: 111 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: V5XDA1
• Locus tag: D174_RS14605
• Protein ID: WP_019512785.1
• Coordinates: 3131789..3131995 (+)
• Length: 69 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
D174_RS14590	3127560..3129542	-	1983	WP_019512789.1	FAD-dependent monooxygenase	-
D174_RS14595	3129637..3130224	+	588	WP_019512788.1	TetR/AcrR family transcriptional regulator	-
D174_RS14600	3130646..3131419	+	774	WP_023985811.1	HAD-IIA family hydrolase	-
D174_RS14605	3131789..3131995	+	207	WP_019512785.1	antitoxin MazE family protein	Antitoxin
D174_RS14610	3131992..3132324	+	333	WP_023985812.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
D174_RS14615	3132589..3133299	+	711	WP_131701367.1	hypothetical protein	-
D174_RS14620	3133758..3134684	-	927	WP_019512782.1	class I SAM-dependent methyltransferase	-
D174_RS14625	3134746..3135318	-	573	WP_019512781.1	TetR/AcrR family transcriptional regulator	-
D174_RS14630	3135448..3135882	-	435	WP_019512780.1	MarR family winged helix-turn-helix transcriptional regulator	-
D174_RS14635	3135945..3136952	+	1008	WP_019512779.1	amidohydrolase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	3122195..3141482	19287
	-	flank	IS/Tn	-	-	3126640..3127497	857

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (2-108)

Antitoxin

• Antitoxin MazE-like (4-66)

Sequences

Toxin

Protein

• Length: 111 a.a.
• Molecular weight: 11770.61 Da
• Isoelectric Point: 6.4638

>T30398 WP_023985812.1 NC_023036:3131992-3132324 [Mycolicibacterium neoaurum VKM Ac-1815D]
VNRGEIWTVAGGVYAAKPRPAVIVQDDLFDATSSVTVAPMTSTLLDAPLMRIRIAGGADLLSGLDHDSDVMIDKLTTVRK
SNVHARVGRLTAEQVVEVERALMAFLGLAR

Nucleotide

• Length: 333 bp

>T30398 NC_023036:3131992-3132324 [Mycolicibacterium neoaurum VKM Ac-1815D]
GTGAACCGAGGAGAGATCTGGACCGTAGCGGGGGGCGTCTATGCAGCGAAGCCGCGTCCGGCAGTGATTGTTCAGGATGA
CCTCTTCGACGCGACAAGTTCAGTGACCGTGGCGCCGATGACCAGCACGCTGTTGGATGCACCTTTGATGCGCATTCGGA
TAGCCGGCGGAGCAGACCTGCTGTCCGGACTTGACCACGACAGTGACGTGATGATCGACAAGCTCACCACCGTGAGAAAA
TCGAATGTCCATGCTCGTGTCGGCCGTCTGACTGCCGAGCAAGTGGTCGAGGTCGAGCGAGCCTTGATGGCGTTCCTCGG
CCTCGCGCGATGA

Antitoxin

Protein

• Length: 69 a.a.
• Molecular weight: 7924.77 Da
• Isoelectric Point: 4.4805

>AT30398 WP_019512785.1 NC_023036:3131789-3131995 [Mycolicibacterium neoaurum VKM Ac-1815D]
VAVRERVGEYRRRMRERGLRPLQVWVPDVRTETFAAEAHRQASLVAQAEDVSDDQDFVEAISVPWDEE

Nucleotide

• Length: 207 bp

>AT30398 NC_023036:3131789-3131995 [Mycolicibacterium neoaurum VKM Ac-1815D]
GTGGCGGTGCGGGAACGGGTTGGCGAGTATCGCCGGCGGATGCGGGAGCGGGGCTTGCGACCTCTGCAGGTCTGGGTGCC
TGATGTGCGGACGGAGACCTTCGCGGCCGAGGCTCACCGTCAGGCGTCGTTGGTTGCGCAAGCCGAAGACGTCAGCGACG
ACCAGGATTTCGTTGAGGCGATCTCGGTGCCTTGGGACGAGGAGTGA

Structures

Toxin

• AlphaFold DB: V5XEF3

Antitoxin

• AlphaFold DB: V5XDA1