Detailed information of TA system TA030393

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): Hha-TomB/-
• Location: 3132777..3133396
• Replicon: chromosome
• Accession: NC_023032
• Organism: Cronobacter sakazakii CMCC 45402

Toxin (Protein)

• Gene name: Hha
• Uniprot ID: V5U1U2
• Locus tag: P262_RS14660
• Protein ID: WP_007781479.1
• Coordinates: 3133178..3133396 (+)
• Length: 73 a.a.

Antitoxin (Protein)

• Gene name: TomB
• Uniprot ID: V5U2H5
• Locus tag: P262_RS14655
• Protein ID: WP_007781481.1
• Coordinates: 3132777..3133151 (+)
• Length: 125 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
P262_RS14645	3127937..3129142	+	1206	WP_007781488.1	multidrug efflux RND transporter periplasmic adaptor subunit AcrA	-
P262_RS14650	3129164..3132313	+	3150	WP_007781486.1	multidrug efflux RND transporter permease subunit	-
P262_RS14655	3132777..3133151	+	375	WP_007781481.1	Hha toxicity modulator TomB	Antitoxin
P262_RS14660	3133178..3133396	+	219	WP_007781479.1	hemolysin expression modulator Hha	Toxin
P262_RS14665	3133601..3134152	+	552	WP_007781478.1	maltose O-acetyltransferase	-
P262_RS14670	3134273..3134782	+	510	WP_007791512.1	YlaC family protein	-
P262_RS22245	3134814..3134954	-	141	WP_004386911.1	type B 50S ribosomal protein L36	-
P262_RS14675	3134958..3135218	-	261	WP_004386912.1	type B 50S ribosomal protein L31	-
P262_RS14680	3135358..3137259	-	1902	WP_007781473.1	methyl-accepting chemotaxis protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Haemolysin expression modulating, HHA (13-69)

Antitoxin

• Biofilm formation regulator YbaJ (1-117)

Sequences

Toxin

Protein

• Length: 73 a.a.
• Molecular weight: 8614.02 Da
• Isoelectric Point: 9.4828

>T30393 WP_007781479.1 NC_023032:3133178-3133396 [Cronobacter malonaticus]
MTTKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFVR

Nucleotide

• Length: 219 bp

>T30393 NC_023032:3133178-3133396 [Cronobacter malonaticus]
ATGACGACCAAACCATTAACTAAAACCGATTATTTAATGCGACTGAGACGCTGTCAGACAATTGACACGCTTGAGCGCGT
TATTGAAAAAAATAAATATGAATTGTCCGACAATGAACTGGCGGTATTTTATTCAGCAGCGGATCATCGTCTGGCTGAAC
TGACCATGAATAAGCTTTACGATAAAATCCCGACCTCCGTGTGGAAATTCGTTCGTTAA

Antitoxin

Protein

• Length: 125 a.a.
• Molecular weight: 14357.08 Da
• Isoelectric Point: 4.7168

>AT30393 WP_007781481.1 NC_023032:3132777-3133151 [Cronobacter malonaticus]
MDEYSPKRHDIAQLKFLCESLYHDCLATLGENQHGWVNDPTSAVNLQLNDLIEHIASFGLNYKIKHEDDAALIEQLDEYL
DDTFMLFSNYGINAQDLQKWRKSGNRLFRCFINASKENPVSLSF

Nucleotide

• Length: 375 bp

>AT30393 NC_023032:3132777-3133151 [Cronobacter malonaticus]
ATGGACGAATACTCACCAAAACGGCATGATATAGCGCAGCTTAAATTTCTCTGTGAGAGCCTGTATCATGATTGCCTCGC
CACGCTAGGCGAAAACCAGCATGGCTGGGTTAACGACCCCACTTCCGCCGTTAACCTGCAACTGAACGACCTGATTGAGC
ATATTGCCAGCTTCGGTCTTAATTATAAAATTAAGCATGAAGATGACGCCGCTCTCATTGAACAATTAGATGAATATCTG
GACGATACCTTTATGCTGTTCAGCAATTACGGTATCAATGCGCAAGATTTACAAAAATGGCGCAAGTCCGGAAACAGGCT
TTTCCGGTGTTTCATTAACGCCAGTAAAGAAAATCCTGTGAGCTTGTCTTTTTAA

Structures

Toxin

• AlphaFold DB: V5U1U2

Antitoxin

• AlphaFold DB: V5U2H5