Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 6960..7603 Replicon plasmid p2
Accession NC_023025
Organism Cronobacter sakazakii CMCC 45402

Toxin (Protein)


Gene name vagD Uniprot ID V5U5J6
Locus tag P262_RS21090 Protein ID WP_007778986.1
Coordinates 6960..7376 (-) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID V5U780
Locus tag P262_RS21095 Protein ID WP_007778988.1
Coordinates 7373..7603 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
P262_RS21065 2227..2631 - 405 WP_000839182.1 IS66 family insertion sequence hypothetical protein -
P262_RS21070 3268..4005 + 738 WP_029039441.1 DUF4145 domain-containing protein -
P262_RS21075 4483..5208 - 726 WP_042391453.1 conjugal transfer protein TraX -
P262_RS23095 5712..6311 - 600 WP_105166309.1 hypothetical protein -
P262_RS21085 6414..6911 - 498 WP_007778984.1 hypothetical protein -
P262_RS21090 6960..7376 - 417 WP_007778986.1 type II toxin-antitoxin system VapC family toxin Toxin
P262_RS21095 7373..7603 - 231 WP_007778988.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
P262_RS21100 7783..7995 + 213 WP_012815904.1 hypothetical protein -
P262_RS21105 8081..8641 - 561 WP_012815905.1 hypothetical protein -
P262_RS21110 8708..10903 - 2196 WP_007778991.1 phage T7 exclusion protein -
P262_RS21115 10904..11989 - 1086 WP_007889785.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..55913 55913


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-48)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 14946.37 Da        Isoelectric Point: 7.8858

>T30378 WP_007778986.1 NC_023025:c7376-6960 [Cronobacter malonaticus]
VKKTYMLDTNICSFIMREQPEAVLKQLEQTVLRGHRIVVSAITYAEMRFGAIGKKASPRHAVLVEAFCARLDAVLAWDRA
AVDATTEIKAALTAAGTPIGPNDTAIAGHAIAAGAVLVTNNVREFERVPGLMLEDWTK

Download         Length: 417 bp

>T30378 NC_023025:c7376-6960 [Cronobacter malonaticus]
GTGAAAAAGACCTATATGCTCGATACCAATATCTGTTCATTCATCATGCGTGAACAGCCCGAAGCGGTACTGAAGCAGCT
GGAGCAGACGGTACTGCGGGGACACCGGATTGTCGTCTCAGCCATTACCTATGCCGAAATGCGGTTCGGTGCCATCGGCA
AAAAAGCCTCCCCGCGTCACGCGGTGCTGGTTGAGGCATTCTGTGCGCGGCTTGATGCCGTTCTGGCGTGGGACCGGGCC
GCGGTGGACGCGACCACAGAAATCAAGGCGGCGCTGACCGCGGCCGGCACCCCGATAGGTCCGAATGATACGGCGATTGC
CGGGCATGCCATTGCAGCCGGCGCCGTACTGGTTACCAATAATGTGCGTGAATTTGAACGTGTTCCGGGACTGATGCTGG
AAGACTGGACGAAATAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8683.80 Da        Isoelectric Point: 4.4432

>AT30378 WP_007778988.1 NC_023025:c7603-7373 [Cronobacter malonaticus]
MRTVSVFKNGQNRAIRLPRDMDFEGVSELEIIREGDSIILRPVRPTWGSFAQLDKADPDFMAERADVVSDEGRFDL

Download         Length: 231 bp

>AT30378 NC_023025:c7603-7373 [Cronobacter malonaticus]
ATGCGTACTGTGTCTGTATTTAAAAATGGCCAGAACCGGGCTATCCGGCTGCCGCGGGATATGGATTTTGAGGGCGTCAG
CGAGCTGGAAATCATCCGGGAAGGCGACAGTATCATCCTGCGTCCCGTCCGCCCGACGTGGGGCTCCTTCGCGCAGCTCG
ACAAAGCAGATCCGGACTTTATGGCGGAGCGCGCCGACGTGGTCAGCGACGAAGGACGATTTGACCTGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7V7URM1

Antitoxin

Source ID Structure
AlphaFold DB A0A7V7RF87

References