Detailed information of TA system TA030377

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-HipB
• Location: 1470..3073
• Replicon: plasmid p1
• Accession: NC_023024
• Organism: Cronobacter sakazakii CMCC 45402

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: V5U5S2
• Locus tag: P262_RS20540
• Protein ID: WP_023897618.1
• Coordinates: 1470..2789 (-)
• Length: 440 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: -
• Locus tag: P262_RS20545
• Protein ID: WP_007781034.1
• Coordinates: 2792..3073 (-)
• Length: 94 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
P262_RS20540	1470..2789	-	1320	WP_023897618.1	type II toxin-antitoxin system HipA family toxin	Toxin
P262_RS20545	2792..3073	-	282	WP_007781034.1	helix-turn-helix domain-containing protein	Antitoxin
P262_RS20550	3206..4090	-	885	WP_023897619.1	siderophore-interacting protein	-
P262_RS20555	4087..5298	-	1212	WP_007780883.1	MFS transporter	-
P262_RS20560	5434..7182	+	1749	WP_032983474.1	aerobactin synthase IucA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	iucA / iucB / iucC / iutA / mgtB	1..126488	126488

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA-like, C-terminal (153-405)

Antitoxin

• Cro/C1-type helix-turn-helix domain (21-69)

Sequences

Toxin

Protein

• Length: 440 a.a.
• Molecular weight: 49008.97 Da
• Isoelectric Point: 7.2132

>T30377 WP_023897618.1 NC_023024:c2789-1470 [Cronobacter malonaticus]
MRRTSRLALWMNGLPVGYWDKHNGEDRLTYLPEWIADPQGRPLSLSLPFTPGNQSYRGARVRDYFDNLLPDSENIRRRLA
MRYRAQSLEPFDLLAELGRDCVGAIQLLPAGEAPEGLFTIRARPLSTVDIAAMLRHTAEAFPGQHGGEEELRLSIAGAQE
KTALLWHEGQWCLPEGNTPTTHIFKLPLGLVGNMQADMRTSIENEWLCAQLLEAWDIPVARTQMAQFEEQKVLVVERFDR
RLSADGQWWLRLPQEDMCQALGVSPLRKYQADGGPGIGDIMTVLSRSENAQADRANFFRAQIIFWILAATDGHAKNFSIA
HLPGNRYHLTPLYDVLSAWPVIGPGNNQIAWQKCKLAMAVRSGSNYYHLSQIRRRHWLRQGELAGLSAAQVNDMIDALTA
SAPAAIARVAGTLPVDFPASLADVIFNGIRQQCARLAAA

Nucleotide

• Length: 1320 bp

>T30377 NC_023024:c2789-1470 [Cronobacter malonaticus]
ATGCGCCGCACGTCGCGTTTAGCGCTCTGGATGAACGGCCTCCCGGTCGGCTACTGGGATAAACACAATGGCGAAGACCG
GCTGACCTATCTGCCGGAGTGGATTGCCGATCCGCAAGGGCGGCCGCTGTCGCTGTCGCTGCCGTTCACGCCCGGTAATC
AGTCATACCGCGGCGCGCGGGTGCGCGATTACTTCGACAACCTGCTGCCGGACAGCGAAAACATCCGGCGGCGGCTGGCG
ATGCGCTACCGGGCGCAGAGCCTGGAGCCGTTCGATCTGCTGGCGGAGCTGGGACGCGACTGCGTCGGCGCTATTCAGCT
CCTGCCCGCCGGTGAAGCGCCGGAAGGGCTGTTTACCATCCGCGCCCGTCCACTCTCCACAGTGGATATTGCCGCGATGC
TGCGCCACACCGCAGAGGCGTTCCCCGGCCAGCATGGCGGCGAAGAGGAGCTGCGGCTCTCCATTGCGGGCGCGCAGGAG
AAAACCGCGCTGCTGTGGCACGAAGGCCAGTGGTGCCTGCCGGAGGGCAACACGCCCACCACGCATATTTTCAAACTGCC
GCTGGGGCTGGTGGGCAATATGCAGGCGGACATGCGCACCTCGATTGAAAACGAATGGCTCTGCGCGCAGCTGCTGGAAG
CCTGGGATATTCCGGTCGCGCGCACGCAGATGGCGCAGTTCGAAGAGCAAAAAGTGCTGGTTGTCGAGCGCTTCGACCGG
CGTCTCTCGGCCGATGGCCAGTGGTGGCTGCGTCTGCCGCAGGAGGATATGTGCCAGGCGCTGGGCGTCTCGCCGCTGCG
TAAATATCAGGCCGACGGCGGGCCGGGCATCGGCGATATCATGACGGTTCTGAGCCGTTCAGAAAACGCGCAGGCGGATC
GCGCGAATTTCTTCCGCGCGCAGATTATCTTCTGGATCCTCGCCGCGACCGACGGCCACGCCAAGAATTTCAGCATCGCG
CATCTGCCCGGCAACCGCTACCATCTGACGCCGCTTTACGACGTGCTTTCCGCCTGGCCGGTCATCGGGCCGGGTAATAA
CCAGATTGCGTGGCAGAAGTGCAAGCTGGCGATGGCGGTGCGCAGCGGCAGTAACTATTACCACCTGAGCCAGATCCGCC
GCCGTCACTGGCTGCGCCAGGGCGAGCTTGCCGGGCTGTCGGCGGCGCAGGTCAACGACATGATTGATGCGCTGACAGCC
AGCGCGCCCGCGGCGATCGCGCGCGTGGCCGGCACGCTGCCGGTGGATTTCCCCGCTTCTCTGGCAGACGTTATTTTTAA
CGGCATACGCCAGCAGTGCGCGCGCCTCGCGGCCGCATAA

Antitoxin

Protein

• Length: 94 a.a.
• Molecular weight: 10473.89 Da
• Isoelectric Point: 8.0115

>AT30377 WP_007781034.1 NC_023024:c3073-2792 [Cronobacter malonaticus]
MNTHYPLKTLSQLRPLLVGFRKNQGLTQKDVSARLGVTQQTYARLEANPETASIERLFRVFNVLGIEMTLCETQAAEADA
PARDDSPARREAW

Nucleotide

• Length: 282 bp

>AT30377 NC_023024:c3073-2792 [Cronobacter malonaticus]
ATGAATACCCATTATCCACTGAAAACGCTCAGCCAGCTGCGCCCGCTGCTGGTGGGCTTTCGTAAAAATCAGGGGCTGAC
CCAGAAAGACGTCTCCGCGCGGCTTGGCGTCACGCAGCAAACCTATGCGCGGCTGGAGGCGAACCCCGAGACGGCCAGCA
TTGAGCGCCTGTTCCGCGTCTTTAACGTGCTGGGCATTGAGATGACGCTGTGCGAAACGCAGGCGGCAGAAGCTGACGCG
CCCGCGCGTGACGATTCGCCTGCCCGTCGCGAGGCGTGGTAA

Structures

Toxin

• AlphaFold DB: V5U5S2