Detailed information of TA system TA030374

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicA-HicB
• Location: 4697440..4698097
• Replicon: chromosome
• Accession: NC_023019
• Organism: Pseudomonas aeruginosa MTB-1

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: A0A8G3IXE4
• Locus tag: U769_RS21700
• Protein ID: WP_003098540.1
• Coordinates: 4697440..4697622 (+)
• Length: 61 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: U769_RS21705
• Protein ID: WP_023875730.1
• Coordinates: 4697669..4698097 (+)
• Length: 143 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
U769_RS21670	4694183..4694728	-	546	WP_023114595.1	terminase small subunit	-
U769_RS21675	4694848..4695591	-	744	WP_023114596.1	hypothetical protein	-
U769_RS21680	4695588..4696058	-	471	WP_023875727.1	lysis system i-spanin subunit Rz	-
U769_RS21685	4696055..4696297	-	243	WP_023875728.1	hypothetical protein	-
U769_RS21690	4696294..4696911	-	618	WP_023875729.1	glycoside hydrolase family 19 protein	-
U769_RS21695	4696908..4697240	-	333	WP_003129668.1	phage holin, lambda family	-
U769_RS21700	4697440..4697622	+	183	WP_003098540.1	type II toxin-antitoxin system HicA family toxin	Toxin
U769_RS21705	4697669..4698097	+	429	WP_023875730.1	transcriptional regulator	Antitoxin
U769_RS21710	4698530..4698919	-	390	WP_004353175.1	hypothetical protein	-
U769_RS21715	4698916..4699194	-	279	WP_015648203.1	hypothetical protein	-
U769_RS21720	4699191..4699880	-	690	WP_023875731.1	metallophosphoesterase	-
U769_RS21725	4699877..4700248	-	372	WP_023124260.1	hypothetical protein	-
U769_RS21730	4700245..4701675	-	1431	WP_023875733.1	replicative DNA helicase	-
U769_RS21735	4701672..4702460	-	789	WP_023875734.1	ATP-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	4674062..4730196	56134

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (4-59)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (7-78)

Sequences

Toxin

Protein

• Length: 61 a.a.
• Molecular weight: 6746.86 Da
• Isoelectric Point: 11.0775

>T30374 WP_003098540.1 NC_023019:4697440-4697622 [Pseudomonas aeruginosa MTB-1]
MKFSEFRRWLKAQGVTFEAGKGSHFKITAPNGKQTTFADHGAKEMPEPTRKAIIKQLGLK

Nucleotide

• Length: 183 bp

>T30374 NC_023019:4697440-4697622 [Pseudomonas aeruginosa MTB-1]
ATGAAGTTCAGCGAATTCAGACGATGGTTGAAGGCCCAAGGGGTGACCTTCGAAGCCGGCAAAGGAAGCCACTTCAAGAT
CACCGCCCCGAACGGCAAACAGACCACCTTCGCGGACCACGGAGCTAAGGAAATGCCAGAACCGACCCGCAAGGCGATCA
TCAAGCAACTGGGGCTCAAATGA

Antitoxin

Protein

• Length: 143 a.a.
• Molecular weight: 15758.92 Da
• Isoelectric Point: 4.6562

>AT30374 WP_023875730.1 NC_023019:4697669-4698097 [Pseudomonas aeruginosa MTB-1]
MYDYAIRFEQDDSAPGVAVFCRDLPELNSYGDDKAHAISEAVDAIESTLSLYVDQRREIPAASQAQPGERVIHLPAVTVA
KIALWNEMVRRDMRKADLCRLLGIAQTQGDRLVDFLHNTKMEAMENALSALGLRLSVNIEAA

Nucleotide

• Length: 429 bp

>AT30374 NC_023019:4697669-4698097 [Pseudomonas aeruginosa MTB-1]
ATGTACGACTATGCAATCCGTTTCGAACAGGACGATAGCGCTCCTGGCGTCGCCGTTTTCTGCAGAGACCTGCCGGAACT
GAACAGCTACGGCGACGATAAGGCCCATGCAATCAGCGAGGCGGTTGACGCCATCGAGTCAACCCTCTCGCTGTATGTTG
ATCAGCGCCGAGAAATCCCCGCAGCCAGCCAGGCACAACCAGGCGAGCGCGTTATCCATCTGCCGGCAGTTACCGTTGCG
AAGATCGCGCTCTGGAACGAAATGGTTCGTCGAGATATGCGAAAGGCTGACCTCTGCCGGCTCCTCGGTATCGCACAAAC
CCAGGGCGACAGGCTCGTCGACTTCCTCCACAACACTAAGATGGAAGCCATGGAGAACGCGCTATCCGCCCTCGGCCTCC
GCCTCTCAGTGAATATTGAGGCGGCATGA