Detailed information of TA system TA030352

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): BrnTA/BrnT-BrnA
• Location: 1044845..1045404
• Replicon: chromosome
• Accession: NC_022907
• Organism: Brucella ceti TE28753-12

Toxin (Protein)

• Gene name: brnT
• Uniprot ID: D6LP74
• Locus tag: MR74_RS05415
• Protein ID: WP_004688321.1
• Coordinates: 1044845..1045090 (+)
• Length: 82 a.a.

Antitoxin (Protein)

• Gene name: brnA
• Uniprot ID: D0RHG9
• Locus tag: MR74_RS05420
• Protein ID: WP_006161014.1
• Coordinates: 1045090..1045404 (+)
• Length: 105 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MR74_RS05390	1040693..1042338	-	1646	Protein_983	hypothetical protein	-
MR74_RS05395	1042473..1043246	-	774	WP_023598555.1	fibronectin type III domain-containing protein	-
MR74_RS05410	1044074..1044712	-	639	Protein_986	tRNA-guanine transglycosylase	-
MR74_RS05415	1044845..1045090	+	246	WP_004688321.1	BrnT family toxin	Toxin
MR74_RS05420	1045090..1045404	+	315	WP_006161014.1	BrnA antitoxin family protein	Antitoxin
MR74_RS05430	1045752..1045922	+	171	WP_002964095.1	DUF1508 domain-containing protein	-
MR74_RS05435	1046270..1046782	-	513	WP_158332263.1	porin family protein	-
MR74_RS16875	1046783..1046981	-	199	Protein_991	porin family protein	-
MR74_RS05440	1047216..1047803	-	588	WP_004683738.1	hypothetical protein	-
MR74_RS05445	1047826..1048101	-	276	WP_002964092.1	hypothetical protein	-
MR74_RS05450	1048098..1048328	-	231	WP_002964091.1	hypothetical protein	-
MR74_RS05455	1048325..1049042	-	718	Protein_995	hypothetical protein	-
MR74_RS05460	1049092..1049295	+	204	WP_002964088.1	hypothetical protein	-
MR74_RS05465	1049292..1049506	+	215	Protein_997	hypothetical protein	-
MR74_RS05470	1049539..1049739	+	201	WP_006641932.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	wbdA / wboA	1038050..1051508	13458

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (5-78)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (28-102)

Sequences

Toxin

Protein

• Length: 82 a.a.
• Molecular weight: 9172.86 Da
• Isoelectric Point: 10.3158

>T30352 WP_004688321.1 NC_022907:1044845-1045090 [Brucella ceti TE28753-12]
MKIIWDEPKRQTNIAKHGLDFADLHFEFFLSAKVFPTKADRLMAIGEFNGLIIIAVIFKPVGSEALSVISMRPASQKERK
L

Nucleotide

• Length: 246 bp

>T30352 NC_022907:1044845-1045090 [Brucella ceti TE28753-12]
ATGAAGATCATCTGGGACGAACCGAAGCGACAGACCAACATTGCCAAGCACGGCTTGGACTTCGCTGACCTGCATTTCGA
ATTCTTCCTGTCGGCTAAGGTCTTCCCCACCAAGGCAGATCGCCTGATGGCAATCGGAGAATTCAACGGCCTGATTATCA
TCGCCGTCATTTTCAAGCCGGTTGGTTCGGAAGCCCTCTCCGTGATCTCCATGCGTCCAGCAAGCCAGAAGGAAAGGAAG
CTCTGA

Antitoxin

Protein

• Length: 105 a.a.
• Molecular weight: 11668.45 Da
• Isoelectric Point: 10.0945

>AT30352 WP_006161014.1 NC_022907:1045090-1045404 [Brucella ceti TE28753-12]
MGIKFSSKRPLTQEEEAEIQKMIASDPDAPEATDEQLAKAKPFKEAFPDMAAKMEKAIRGWPRIDNPKTPVTIRLDQDVV
QRFKATGKGWQGRMNDALRKAVGL

Nucleotide

• Length: 315 bp

>AT30352 NC_022907:1045090-1045404 [Brucella ceti TE28753-12]
ATGGGTATCAAATTTTCCTCTAAGCGCCCTCTCACTCAGGAAGAAGAGGCAGAAATTCAGAAGATGATCGCAAGCGATCC
CGATGCACCGGAAGCCACCGATGAACAGCTTGCCAAGGCCAAGCCTTTCAAGGAAGCCTTTCCAGATATGGCCGCCAAGA
TGGAGAAGGCCATTCGAGGCTGGCCGCGCATCGACAATCCCAAGACGCCGGTTACTATCCGGCTCGATCAGGATGTAGTT
CAACGCTTCAAGGCCACCGGCAAAGGCTGGCAGGGCCGTATGAACGATGCCCTGCGCAAAGCTGTCGGGCTTTGA

Structures

Antitoxin

• AlphaFold DB: D0RHG9