Detailed information of TA system TA030351

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): BrnTA/BrnT-BrnA
• Location: 1045642..1046201
• Replicon: chromosome
• Accession: NC_022905
• Organism: Brucella ceti TE10759-12

Toxin (Protein)

• Gene name: brnT
• Uniprot ID: D6LP74
• Locus tag: V910_RS05010
• Protein ID: WP_004688321.1
• Coordinates: 1045642..1045887 (+)
• Length: 82 a.a.

Antitoxin (Protein)

• Gene name: brnA
• Uniprot ID: D0RHG9
• Locus tag: V910_RS05015
• Protein ID: WP_006161014.1
• Coordinates: 1045887..1046201 (+)
• Length: 105 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
V910_RS04985	1041489..1043135	-	1647	WP_004689673.1	hypothetical protein	-
V910_RS04990	1043270..1044043	-	774	WP_023598555.1	fibronectin type III domain-containing protein	-
V910_RS05005	1044871..1045509	-	639	Protein_981	tRNA-guanine transglycosylase	-
V910_RS05010	1045642..1045887	+	246	WP_004688321.1	BrnT family toxin	Toxin
V910_RS05015	1045887..1046201	+	315	WP_006161014.1	BrnA antitoxin family protein	Antitoxin
V910_RS05020	1046549..1046719	+	171	WP_002964095.1	DUF1508 domain-containing protein	-
V910_RS16280	1047067..1047579	-	513	WP_158332263.1	porin family protein	-
V910_RS17720	1047580..1047778	-	199	Protein_986	porin family protein	-
V910_RS05035	1048013..1048600	-	588	WP_004683738.1	hypothetical protein	-
V910_RS05040	1048623..1048898	-	276	WP_002964092.1	hypothetical protein	-
V910_RS05045	1048895..1049125	-	231	WP_002964091.1	hypothetical protein	-
V910_RS05050	1049122..1049841	-	720	WP_006072696.1	hypothetical protein	-
V910_RS05055	1049891..1050094	+	204	WP_002964088.1	hypothetical protein	-
V910_RS05060	1050091..1050306	+	216	WP_002971459.1	hypothetical protein	-
V910_RS05065	1050319..1050519	+	201	WP_006641932.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	wbdA / wboA	1038375..1052290	13915

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (5-78)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (28-102)

Sequences

Toxin

Protein

• Length: 82 a.a.
• Molecular weight: 9172.86 Da
• Isoelectric Point: 10.3158

>T30351 WP_004688321.1 NC_022905:1045642-1045887 [Brucella ceti TE10759-12]
MKIIWDEPKRQTNIAKHGLDFADLHFEFFLSAKVFPTKADRLMAIGEFNGLIIIAVIFKPVGSEALSVISMRPASQKERK
L

Nucleotide

• Length: 246 bp

>T30351 NC_022905:1045642-1045887 [Brucella ceti TE10759-12]
ATGAAGATCATCTGGGACGAACCGAAGCGACAGACCAACATTGCCAAGCACGGCTTGGACTTCGCTGACCTGCATTTCGA
ATTCTTCCTGTCGGCTAAGGTCTTCCCCACCAAGGCAGATCGCCTGATGGCAATCGGAGAATTCAACGGCCTGATTATCA
TCGCCGTCATTTTCAAGCCGGTTGGTTCGGAAGCCCTCTCCGTGATCTCCATGCGTCCAGCAAGCCAGAAGGAAAGGAAG
CTCTGA

Antitoxin

Protein

• Length: 105 a.a.
• Molecular weight: 11668.45 Da
• Isoelectric Point: 10.0945

>AT30351 WP_006161014.1 NC_022905:1045887-1046201 [Brucella ceti TE10759-12]
MGIKFSSKRPLTQEEEAEIQKMIASDPDAPEATDEQLAKAKPFKEAFPDMAAKMEKAIRGWPRIDNPKTPVTIRLDQDVV
QRFKATGKGWQGRMNDALRKAVGL

Nucleotide

• Length: 315 bp

>AT30351 NC_022905:1045887-1046201 [Brucella ceti TE10759-12]
ATGGGTATCAAATTTTCCTCTAAGCGCCCTCTCACTCAGGAAGAAGAGGCAGAAATTCAGAAGATGATCGCAAGCGATCC
CGATGCACCGGAAGCCACCGATGAACAGCTTGCCAAGGCCAAGCCTTTCAAGGAAGCCTTTCCAGATATGGCCGCCAAGA
TGGAGAAGGCCATTCGAGGCTGGCCGCGCATCGACAATCCCAAGACGCCGGTTACTATCCGGCTCGATCAGGATGTAGTT
CAACGCTTCAAGGCCACCGGCAAAGGCTGGCAGGGCCGTATGAACGATGCCCTGCGCAAAGCTGTCGGGCTTTGA

Structures

Antitoxin

• AlphaFold DB: D0RHG9