Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 502200..502836 Replicon chromosome
Accession NC_022898
Organism Bacillus subtilis PY79

Toxin (Protein)


Gene name mazF Uniprot ID G4NU33
Locus tag U712_RS02520 Protein ID WP_003156187.1
Coordinates 502486..502836 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID G4NU32
Locus tag U712_RS02515 Protein ID WP_003225183.1
Coordinates 502200..502481 (+) Length 94 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
U712_RS02495 498559..499158 - 600 WP_003246687.1 rhomboid family intramembrane serine protease -
U712_RS02500 499253..499618 + 366 WP_003234281.1 holo-ACP synthase -
U712_RS02505 499784..500800 + 1017 WP_003234282.1 outer membrane lipoprotein carrier protein LolA -
U712_RS02510 500915..502084 + 1170 WP_003234284.1 alanine racemase -
U712_RS02515 502200..502481 + 282 WP_003225183.1 type II toxin-antitoxin system antitoxin EndoAI Antitoxin
U712_RS02520 502486..502836 + 351 WP_003156187.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
U712_RS02525 502951..503775 + 825 WP_009966610.1 RsbT co-antagonist protein RsbRA -
U712_RS02530 503780..504145 + 366 WP_003225190.1 RsbT antagonist protein RsbS -
U712_RS02535 504149..504550 + 402 WP_003246640.1 serine/threonine-protein kinase RsbT -
U712_RS02540 504562..505569 + 1008 WP_003234295.1 phosphoserine phosphatase RsbU -
U712_RS02545 505631..505960 + 330 WP_003234298.1 anti-sigma factor antagonist RsbV -
U712_RS02550 505957..506439 + 483 WP_003234299.1 anti-sigma B factor RsbW -
U712_RS02555 506405..507193 + 789 WP_003246715.1 RNA polymerase sigma factor SigB -
U712_RS02560 507193..507792 + 600 WP_003246608.1 phosphoserine phosphatase RsbX -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin

Ribbon-helix-helix protein, CopG

(12-45)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12977.98 Da        Isoelectric Point: 4.8781

>T30337 WP_003156187.1 NC_022898:502486-502836 [Bacillus subtilis PY79]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Download         Length: 351 bp

>T30337 NC_022898:502486-502836 [Bacillus subtilis PY79]
TTGATTGTGAAACGCGGCGATGTTTATTTTGCTGATTTATCTCCTGTTGTTGGCTCAGAGCAAGGCGGGGTGCGCCCGGT
TTTAGTGATCCAAAATGACATCGGAAATCGCTTCAGCCCAACTGCTATTGTTGCAGCCATAACAGCACAAATACAGAAAG
CGAAATTACCAACCCACGTCGAAATCGATGCAAAACGCTACGGTTTTGAAAGAGATTCCGTTATTTTGCTGGAGCAAATT
CGGACGATTGACAAGCAAAGGTTAACGGATAAGATTACTCATCTGGATGATGAAATGATGGATAAGGTTGATGAAGCCTT
ACAAATCAGTTTGGCACTCATTGATTTTTAG

Antitoxin


Download         Length: 94 a.a.        Molecular weight: 10546.92 Da        Isoelectric Point: 6.4708

>AT30337 WP_003225183.1 NC_022898:502200-502481 [Bacillus subtilis PY79]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHFAEC
EAETTVERLVSGG

Download         Length: 282 bp

>AT30337 NC_022898:502200-502481 [Bacillus subtilis PY79]
TTGTCTGAATCCAGCGCAAGAACCGAAATGAAAATCAGCTTGCCCGAAAACCTAGTAGCTGAATTGGATGGTGTAGCGAT
GCGGGAGAAACGAAGCAGAAACGAACTGATATCACAAGCAGTGAGAGCGTATGTCAGCGAACGAACAACTCGTCACAACC
GTGATTTGATGAGACGCGGCTATATGGAAATGGCGAAAATCAACCTGAATATTTCTTCTGAGGCTCACTTTGCGGAGTGC
GAGGCTGAAACGACAGTTGAGCGCTTAGTCAGCGGAGGATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4MDX
PDB 4ME7
PDB 1NE8

Antitoxin

Source ID Structure
PDB 4ME7
AlphaFold DB G4NU32

References