Detailed information of TA system TA030334

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RelE(toxin)
• Location: 143071..143552
• Replicon: plasmid pQY182
• Accession: NC_022879
• Organism: Enterococcus mundtii QU 25

Toxin (Protein)

• Gene name: relE
• Uniprot ID: V5XUP7
• Locus tag: EMQU_RS14980
• Protein ID: WP_023520737.1
• Coordinates: 143283..143552 (+)
• Length: 90 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: V5XSV2
• Locus tag: EMQU_RS14975
• Protein ID: WP_023520736.1
• Coordinates: 143071..143286 (+)
• Length: 72 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EMQU_RS14935	138809..139111	+	303	Protein_129	DNA/RNA non-specific endonuclease	-
EMQU_RS14940	139550..139990	+	441	WP_023520729.1	JAB domain-containing protein	-
EMQU_RS14945	140055..140510	+	456	WP_023520730.1	PcfK-like family protein	-
EMQU_RS14950	140507..141847	+	1341	WP_023520731.1	PcfJ domain-containing protein	-
EMQU_RS14955	142006..142188	+	183	WP_023520732.1	hypothetical protein	-
EMQU_RS14960	142172..142381	+	210	WP_023520733.1	hypothetical protein	-
EMQU_RS14965	142519..142767	+	249	WP_023520734.1	hypothetical protein	-
EMQU_RS14970	142773..143009	+	237	WP_148294505.1	hypothetical protein	-
EMQU_RS14975	143071..143286	+	216	WP_023520736.1	hypothetical protein	Antitoxin
EMQU_RS14980	143283..143552	+	270	WP_023520737.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
EMQU_RS14985	144330..147212	+	2883	WP_023520738.1	BspA family leucine-rich repeat surface protein	-
EMQU_RS14990	147248..147766	+	519	WP_023520739.1	hypothetical protein	-
EMQU_RS14995	147791..148354	+	564	WP_023520740.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..181920	181920

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-83)

Antitoxin

• Protein of unknown function DUF6290 (1-67)

Sequences

Toxin

Protein

• Length: 90 a.a.
• Molecular weight: 10601.43 Da
• Isoelectric Point: 10.1737

>T30334 WP_023520737.1 NC_022879:143283-143552 [Enterococcus mundtii QU 25]
MKYHVEFDKRAQKEFLKLDKPIRKQILLWLNKNIEGCENPRWTGKGLTADKSGLWRYRIGKYRLICDIRDDYALVLVIKA
GKRETIYDG

Nucleotide

• Length: 270 bp

>T30334 NC_022879:143283-143552 [Enterococcus mundtii QU 25]
ATGAAGTATCACGTTGAATTTGATAAACGCGCACAAAAAGAATTTTTGAAGTTAGATAAACCCATTCGAAAGCAGATTCT
GCTTTGGTTGAATAAGAATATCGAGGGTTGTGAAAATCCGAGATGGACTGGAAAAGGACTTACCGCTGATAAAAGTGGCT
TGTGGCGATATCGAATAGGGAAATATAGATTAATATGTGATATTCGTGATGATTATGCTTTGGTTCTAGTTATAAAAGCT
GGCAAGCGAGAAACGATTTATGACGGCTAG

Antitoxin

Protein

• Length: 72 a.a.
• Molecular weight: 7872.92 Da
• Isoelectric Point: 4.0863

>AT30334 WP_023520736.1 NC_022879:143071-143286 [Enterococcus mundtii QU 25]
MSVTTIRLNEQEEAFFQSYAELTGQPLSTLMKQALTEKIEDYLDLQAGSEALKNLSGESVSLKDMMKAEGL

Nucleotide

• Length: 216 bp

>AT30334 NC_022879:143071-143286 [Enterococcus mundtii QU 25]
ATGAGCGTTACAACGATAAGATTGAATGAACAAGAAGAGGCATTTTTTCAAAGCTATGCTGAGTTAACTGGTCAACCTTT
ATCAACTTTAATGAAACAAGCATTAACTGAAAAAATTGAAGATTACCTGGACTTGCAAGCGGGATCTGAAGCTCTAAAGA
ATCTATCTGGTGAATCTGTCTCTCTTAAAGACATGATGAAGGCTGAAGGTTTATGA

Structures

Toxin

• AlphaFold DB: V5XUP7

Antitoxin

• AlphaFold DB: V5XSV2