Detailed information of TA system TA030332

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PRK09907(toxin)
• Location: 95974..96545
• Replicon: plasmid pQY182
• Accession: NC_022879
• Organism: Enterococcus mundtii QU 25

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: V5XTY4
• Locus tag: EMQU_RS14690
• Protein ID: WP_023520686.1
• Coordinates: 96204..96545 (+)
• Length: 114 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: V5XUJ2
• Locus tag: EMQU_RS14685
• Protein ID: WP_023520685.1
• Coordinates: 95974..96204 (+)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EMQU_RS16555	91816..94641	+	2826	WP_080674338.1	BspA family leucine-rich repeat surface protein	-
EMQU_RS14675	94807..95130	+	324	WP_023520683.1	hypothetical protein	-
EMQU_RS14680	95159..95734	-	576	WP_023520684.1	recombinase family protein	-
EMQU_RS14685	95974..96204	+	231	WP_023520685.1	hypothetical protein	Antitoxin
EMQU_RS14690	96204..96545	+	342	WP_023520686.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
EMQU_RS14695	96796..97026	+	231	WP_041684655.1	hypothetical protein	-
EMQU_RS14700	97086..98339	+	1254	WP_023520605.1	efflux RND transporter periplasmic adaptor subunit	-
EMQU_RS14705	98326..99009	+	684	WP_023519171.1	ABC transporter ATP-binding protein	-
EMQU_RS14710	99016..100247	+	1232	Protein_86	ABC transporter permease	-
EMQU_RS14715	100260..100916	+	657	WP_023520608.1	response regulator transcription factor	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..181920	181920
	-	flank	IS/Tn	-	-	95159..95734	575

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (10-113)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 13210.52 Da
• Isoelectric Point: 9.9049

>T30332 WP_023520686.1 NC_022879:96204-96545 [Enterococcus mundtii QU 25]
VLEDKNYIPKKGDIVWIDFDPLIGNEIQKRRPGLVVSRYEFNRKTMFAVICPITSTIKNLPTRYSLPEDLDTNGQVLISQ
LKSLDFKKRKLKKVENLPLQDMAKIDQIIQYIF

Nucleotide

• Length: 342 bp

>T30332 NC_022879:96204-96545 [Enterococcus mundtii QU 25]
GTGTTAGAAGATAAAAATTACATTCCTAAAAAAGGGGATATAGTTTGGATTGATTTTGATCCTTTAATAGGAAACGAAAT
TCAAAAGAGACGTCCAGGATTAGTTGTTTCTCGATATGAGTTTAATCGGAAGACAATGTTTGCGGTAATTTGTCCAATTA
CTTCTACTATAAAGAATCTACCGACGCGCTATTCTCTTCCTGAAGATTTAGATACAAATGGGCAAGTACTTATTTCGCAA
TTAAAATCACTTGATTTCAAGAAAAGAAAACTCAAAAAGGTAGAAAATTTACCTTTACAAGATATGGCTAAAATTGATCA
GATTATCCAGTATATATTTTAA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8523.39 Da
• Isoelectric Point: 3.8852

>AT30332 WP_023520685.1 NC_022879:95974-96204 [Enterococcus mundtii QU 25]
MLITKSRLQGSSVVITLPSDNGKKPSDNQEYIVVYSDDGTITLVPKIDDPFSGGEEAEYYEKDEWEDLTPEGREIL

Nucleotide

• Length: 231 bp

>AT30332 NC_022879:95974-96204 [Enterococcus mundtii QU 25]
ATGCTTATTACAAAATCTAGATTACAAGGAAGTTCTGTAGTGATTACTCTTCCGTCTGATAATGGTAAAAAACCTTCAGA
TAACCAAGAATATATCGTTGTATATTCTGATGATGGAACAATTACGTTAGTTCCTAAAATCGATGATCCATTTAGTGGAG
GAGAAGAAGCTGAGTATTATGAAAAAGATGAATGGGAAGATTTAACTCCTGAAGGGAGAGAAATTTTATAG

Structures

Toxin

• AlphaFold DB: V5XTY4

Antitoxin

• AlphaFold DB: V5XUJ2