Detailed information of TA system TA030331

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicA-HicB
• Location: 72444..73053
• Replicon: plasmid pQY182
• Accession: NC_022879
• Organism: Enterococcus mundtii QU 25

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: -
• Locus tag: EMQU_RS14575
• Protein ID: WP_023520664.1
• Coordinates: 72444..72632 (+)
• Length: 63 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: V5XUH4
• Locus tag: EMQU_RS14580
• Protein ID: WP_023520665.1
• Coordinates: 72667..73053 (+)
• Length: 129 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EMQU_RS14550	68312..69985	+	1674	WP_023520660.1	hypothetical protein	-
EMQU_RS14555	69969..70250	+	282	WP_023520661.1	hypothetical protein	-
EMQU_RS14565	70488..70874	+	387	WP_023520662.1	plasmid mobilization relaxosome protein MobC	-
EMQU_RS14570	70876..72378	+	1503	WP_023520663.1	relaxase/mobilization nuclease domain-containing protein	-
EMQU_RS14575	72444..72632	+	189	WP_023520664.1	type II toxin-antitoxin system HicA family toxin	Toxin
EMQU_RS14580	72667..73053	+	387	WP_023520665.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
EMQU_RS14585	73046..73855	+	810	WP_080674337.1	Fic family protein	-
EMQU_RS14590	73956..74622	+	667	Protein_63	DNA/RNA non-specific endonuclease	-
EMQU_RS14595	74909..75121	+	213	WP_023520668.1	hypothetical protein	-
EMQU_RS14600	75513..76685	+	1173	WP_041684687.1	hypothetical protein	-
EMQU_RS14605	77629..77904	+	276	WP_041684689.1	LPXTG cell wall anchor domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..181920	181920

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (6-60)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (5-100)

Sequences

Toxin

Protein

• Length: 63 a.a.
• Molecular weight: 7019.30 Da
• Isoelectric Point: 11.3094

>T30331 WP_023520664.1 NC_022879:72444-72632 [Enterococcus mundtii QU 25]
MPMTIREAEKLLKDAGFVEVKGGKGSHRKFIKKNFPRPVILTSHSKELSRRVEDSVRKAVGK

Nucleotide

• Length: 189 bp

>T30331 NC_022879:72444-72632 [Enterococcus mundtii QU 25]
ATGCCCATGACAATTAGAGAAGCAGAGAAATTACTCAAAGATGCTGGCTTTGTTGAAGTGAAGGGTGGAAAGGGGTCACA
CAGGAAATTTATAAAGAAAAATTTTCCTAGACCAGTTATTCTTACCAGTCATTCAAAAGAGTTATCTAGAAGAGTAGAGG
ATTCTGTTCGAAAAGCAGTGGGCAAATAA

Antitoxin

Protein

• Length: 129 a.a.
• Molecular weight: 14064.19 Da
• Isoelectric Point: 4.3217

>AT30331 WP_023520665.1 NC_022879:72667-73053 [Enterococcus mundtii QU 25]
MKKVYPAIFEKDPVGYGIYFPDIEGAVTQSEDIAQGLELAADALGIMLGDLVEKNMPLPTPTAINALSVNPETEFATLIS
VDLNDYLKDVQLDKKTIKIPHWLNVRATNEGINFSKTMTEALAQKLNV

Nucleotide

• Length: 387 bp

>AT30331 NC_022879:72667-73053 [Enterococcus mundtii QU 25]
ATGAAAAAAGTTTATCCAGCTATATTTGAAAAAGATCCTGTTGGATACGGAATTTATTTTCCTGATATTGAAGGAGCGGT
CACGCAATCGGAAGATATCGCCCAAGGATTAGAACTTGCGGCAGATGCGTTAGGTATTATGCTTGGAGACCTAGTCGAAA
AAAACATGCCTTTGCCAACACCTACAGCTATCAATGCCCTTTCTGTAAACCCTGAAACAGAATTTGCGACATTGATTTCT
GTCGACTTAAATGATTATTTAAAAGATGTCCAACTTGATAAAAAAACAATTAAAATTCCACATTGGTTAAACGTGCGTGC
GACGAATGAAGGAATTAATTTTTCTAAAACGATGACTGAAGCATTAGCTCAAAAATTAAATGTCTGA

Structures

Antitoxin

• AlphaFold DB: V5XUH4