Detailed information of TA system TA030330

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RelE(toxin)
• Location: 2666022..2666512
• Replicon: chromosome
• Accession: NC_022878
• Organism: Enterococcus mundtii QU 25

Toxin (Protein)

• Gene name: relE
• Uniprot ID: V5XU14
• Locus tag: EMQU_RS12610
• Protein ID: WP_010734226.1
• Coordinates: 2666022..2666294 (-)
• Length: 91 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: V5XRI7
• Locus tag: EMQU_RS12615
• Protein ID: WP_010734225.1
• Coordinates: 2666291..2666512 (-)
• Length: 74 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EMQU_RS12595	2661433..2663199	-	1767	WP_010734229.1	aspartate--tRNA ligase	-
EMQU_RS12600	2663199..2664506	-	1308	WP_023520426.1	histidine--tRNA ligase	-
EMQU_RS12605	2664914..2665864	+	951	WP_023520427.1	aldo/keto reductase	-
EMQU_RS12610	2666022..2666294	-	273	WP_010734226.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
EMQU_RS12615	2666291..2666512	-	222	WP_010734225.1	hypothetical protein	Antitoxin
EMQU_RS12620	2666605..2667864	-	1260	WP_010734224.1	cytochrome P450	-
EMQU_RS12625	2668444..2668890	-	447	WP_023520428.1	D-tyrosyl-tRNA(Tyr) deacylase	-
EMQU_RS12630	2668902..2671115	-	2214	WP_010734222.1	bifunctional (p)ppGpp synthetase/guanosine-3',5'-bis(diphosphate) 3'-pyrophosphohydrolase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (7-89)

Antitoxin

• Protein of unknown function DUF6290 (1-71)

Sequences

Toxin

Protein

• Length: 91 a.a.
• Molecular weight: 10642.34 Da
• Isoelectric Point: 10.3153

>T30330 WP_010734226.1 NC_022878:c2666294-2666022 [Enterococcus mundtii QU 25]
MRKQYLVRYEKGAQKALKKMDKFQASLILSWIEKNLINTDNPRQYGKGLVANRSGEWRYRVGNYRIIADIDDEVITILIV
QIGHRNDIYK

Nucleotide

• Length: 273 bp

>T30330 NC_022878:c2666294-2666022 [Enterococcus mundtii QU 25]
ATGAGGAAGCAATATCTTGTCCGTTATGAAAAAGGTGCACAAAAAGCATTGAAAAAAATGGATAAATTTCAAGCTTCTTT
GATACTATCATGGATAGAAAAAAATCTTATCAATACAGATAACCCCAGACAATATGGTAAAGGGTTAGTAGCGAATAGGA
GTGGCGAATGGCGTTATAGAGTGGGGAATTATCGGATCATTGCAGATATAGATGATGAAGTGATAACGATTTTGATCGTA
CAAATTGGTCATCGAAATGACATCTATAAATAG

Antitoxin

Protein

• Length: 74 a.a.
• Molecular weight: 8382.54 Da
• Isoelectric Point: 4.2571

>AT30330 WP_010734225.1 NC_022878:c2666512-2666291 [Enterococcus mundtii QU 25]
MSNITFRVSDEEKAFMLAMADLNGMTVSELARTTLLETLEDQIDMDIYNKAMKDHKSLDESISHEEMKRELGL

Nucleotide

• Length: 222 bp

>AT30330 NC_022878:c2666512-2666291 [Enterococcus mundtii QU 25]
ATGAGTAATATTACGTTTCGTGTATCAGACGAAGAAAAAGCATTTATGTTAGCAATGGCTGATTTGAATGGAATGACCGT
TTCAGAATTGGCAAGGACAACTTTATTAGAAACGCTAGAAGATCAGATTGATATGGATATCTATAATAAAGCGATGAAAG
ATCATAAGAGTCTTGATGAAAGTATCTCGCATGAAGAAATGAAAAGAGAACTAGGATTATGA

Structures

Toxin

• AlphaFold DB: V5XU14

Antitoxin

• AlphaFold DB: V5XRI7