Detailed information of TA system TA030329

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicA-HicB
• Location: 2657819..2658428
• Replicon: chromosome
• Accession: NC_022878
• Organism: Enterococcus mundtii QU 25

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: -
• Locus tag: EMQU_RS12580
• Protein ID: WP_010734231.1
• Coordinates: 2658240..2658428 (-)
• Length: 63 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: V5XTD1
• Locus tag: EMQU_RS12575
• Protein ID: WP_010734232.1
• Coordinates: 2657819..2658205 (-)
• Length: 129 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EMQU_RS12555	2653593..2654228	-	636	WP_010734236.1	1-acyl-sn-glycerol-3-phosphate acyltransferase	-
EMQU_RS12560	2654360..2655094	+	735	WP_010734235.1	tRNA1(Val) (adenine(37)-N6)-methyltransferase	-
EMQU_RS12565	2655084..2655371	+	288	WP_010734234.1	GIY-YIG nuclease family protein	-
EMQU_RS12570	2655493..2657424	-	1932	WP_010734233.1	acetyltransferase	-
EMQU_RS12575	2657819..2658205	-	387	WP_010734232.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
EMQU_RS12580	2658240..2658428	-	189	WP_010734231.1	type II toxin-antitoxin system HicA family toxin	Toxin
EMQU_RS16845	2659954..2660100	-	147	WP_023520425.1	hypothetical protein	-
EMQU_RS12590	2660350..2661225	-	876	WP_010734230.1	YitT family protein	-
EMQU_RS12595	2661433..2663199	-	1767	WP_010734229.1	aspartate--tRNA ligase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (6-62)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (5-101)

Sequences

Toxin

Protein

• Length: 63 a.a.
• Molecular weight: 6983.18 Da
• Isoelectric Point: 11.0565

>T30329 WP_010734231.1 NC_022878:c2658428-2658240 [Enterococcus mundtii QU 25]
MPMTIREAEKLLKDAGFVEVKGGKGSHRKFTKKDHPRPVILTSHSKELSRRVEDSVRKAVGI

Nucleotide

• Length: 189 bp

>T30329 NC_022878:c2658428-2658240 [Enterococcus mundtii QU 25]
ATGCCCATGACAATCAGAGAAGCAGAAAAGCTACTGAAAGATGCTGGCTTTGTTGAAGTGAAAGGTGGGAAGGGCTCACA
TAGGAAATTCACTAAAAAGGATCATCCTAGACCTGTTATCCTTACTAGCCATTCAAAAGAGTTATCTAGAAGAGTAGAAG
ATTCTGTTCGAAAAGCAGTGGGCATTTAA

Antitoxin

Protein

• Length: 129 a.a.
• Molecular weight: 14293.41 Da
• Isoelectric Point: 4.4851

>AT30329 WP_010734232.1 NC_022878:c2658205-2657819 [Enterococcus mundtii QU 25]
MKKVYPAIFEKDPVGYGIYFPDIEGAVTQSKDIIEGLEVASDALGIMLGDLVENNKPLPKPTNINDLSIDSEREFATLIS
VDLNDYLKDVQLDKKTIKIPHWLNVRATNEGINFSKTMSEALVQKLNI

Nucleotide

• Length: 387 bp

>AT30329 NC_022878:c2658205-2657819 [Enterococcus mundtii QU 25]
ATGAAAAAAGTTTATCCAGCTATTTTTGAAAAGGATCCTGTTGGATATGGAATTTATTTTCCTGATATCGAAGGAGCAGT
GACACAATCAAAAGATATTATCGAGGGGTTAGAAGTTGCATCGGATGCTTTAGGGATCATGCTCGGTGATTTAGTTGAGA
ATAATAAACCTTTGCCAAAGCCAACTAATATCAATGATCTTTCGATTGATTCTGAGCGTGAATTTGCTACTTTGATTTCT
GTGGATCTAAATGATTACTTAAAAGATGTCCAACTAGATAAAAAGACAATAAAAATTCCTCATTGGTTAAATGTGCGCGC
AACAAATGAAGGGATAAATTTTTCAAAAACAATGTCTGAAGCTTTGGTTCAAAAATTAAATATATGA

Structures

Antitoxin

• AlphaFold DB: V5XTD1