Detailed information of TA system TA030328

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /SpoIISA(toxin)
• Location: 2642197..2643175
• Replicon: chromosome
• Accession: NC_022873
• Organism: Bacillus thuringiensis YBT-1518

Toxin (Protein)

• Gene name: spoIISA
• Uniprot ID: -
• Locus tag: YBT1518_RS13500
• Protein ID: WP_023521938.1
• Coordinates: 2642197..2642934 (+)
• Length: 246 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: W8YZL5
• Locus tag: YBT1518_RS13505
• Protein ID: WP_000237818.1
• Coordinates: 2643047..2643175 (+)
• Length: 43 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
YBT1518_RS13480	2637839..2638621	+	783	WP_000355672.1	class I SAM-dependent methyltransferase	-
YBT1518_RS13485	2638779..2640464	-	1686	WP_043924657.1	alpha-keto acid decarboxylase family protein	-
YBT1518_RS13490	2640572..2641054	+	483	WP_000191889.1	MarR family winged helix-turn-helix transcriptional regulator	-
YBT1518_RS13495	2641221..2641958	+	738	WP_000594152.1	2,3-diphosphoglycerate-dependent phosphoglycerate mutase	-
YBT1518_RS13500	2642197..2642934	+	738	WP_023521938.1	type II toxin-antitoxin system SpoIISA family toxin	Toxin
YBT1518_RS13505	2643047..2643175	+	129	WP_000237818.1	hypothetical protein	Antitoxin
YBT1518_RS13510	2643248..2643424	+	177	WP_000852629.1	stage II sporulation protein SB	-
YBT1518_RS13515	2643444..2643833	-	390	WP_023521939.1	YxeA family protein	-
YBT1518_RS13520	2644045..2645514	+	1470	WP_023521940.1	beta-Ala-His dipeptidase	-
YBT1518_RS13525	2645760..2646569	+	810	WP_023521941.1	C39 family peptidase	-
YBT1518_RS13530	2646595..2647197	+	603	WP_023521942.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin SpoIISA, type II toxin-antitoxin system (10-243)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 246 a.a.
• Molecular weight: 28309.00 Da
• Isoelectric Point: 8.2998

>T30328 WP_023521938.1 NC_022873:2642197-2642934 [Bacillus thuringiensis YBT-1518]
MISNIRIGLFVLAIVFVVLVFFYWKNEELYEEKKQRIRKTWYGLFIISVTVYFMIKGIDLTLWKNLLMFTAMVIFVDIAF
ILTPNISEIWGAKFSDIGKTVQSIKRSLIASKARGEIYTTIIQNVNPSVFGTMEWHTEEEYTKSLNAFLDSYGEKIGAKI
VVFEAAKELNTNFRGIRSQFSIIVPLEHIEQLNEQKAVQVENVGVIPAKIVSDVFIVIDGKKNNLQDRDFENVYNLTIHH
SYFSK

Nucleotide

• Length: 738 bp

>T30328 NC_022873:2642197-2642934 [Bacillus thuringiensis YBT-1518]
ATGATCTCTAACATTCGAATTGGCCTATTTGTTTTAGCGATTGTCTTTGTAGTCCTTGTTTTCTTTTATTGGAAAAATGA
GGAGTTGTACGAGGAGAAAAAGCAACGTATTAGAAAGACTTGGTATGGTTTGTTTATCATATCTGTCACCGTGTATTTCA
TGATAAAAGGAATTGATTTAACCCTCTGGAAAAATCTTTTAATGTTTACTGCAATGGTTATTTTTGTTGATATTGCGTTC
ATTTTAACACCTAATATTTCAGAAATATGGGGTGCGAAATTTAGTGATATTGGCAAGACAGTTCAATCAATAAAACGATC
ATTAATTGCATCTAAAGCGAGAGGAGAAATATACACGACAATTATTCAAAATGTTAATCCATCAGTATTCGGTACGATGG
AATGGCATACGGAAGAGGAATATACAAAGAGCTTAAATGCATTTTTAGATTCATATGGGGAAAAGATTGGTGCGAAAATT
GTTGTTTTTGAAGCCGCGAAGGAATTAAATACAAACTTTCGTGGTATTCGCTCGCAATTTAGTATTATCGTTCCGTTAGA
ACATATCGAGCAATTGAATGAGCAGAAAGCGGTGCAAGTAGAAAATGTCGGGGTTATACCAGCAAAAATAGTAAGTGACG
TTTTCATTGTTATTGATGGAAAGAAAAATAACCTGCAAGATCGGGATTTTGAAAATGTATATAATTTAACAATACATCAT
AGTTATTTTAGTAAATAA

Antitoxin

Protein

• Length: 43 a.a.
• Molecular weight: 5011.40 Da
• Isoelectric Point: 3.9150

>AT30328 WP_000237818.1 NC_022873:2643047-2643175 [Bacillus thuringiensis YBT-1518]
VVKDYDNDFFKEDNEDTTDFSLIKGDTLQQVEELEEELKRKS

Nucleotide

• Length: 129 bp

>AT30328 NC_022873:2643047-2643175 [Bacillus thuringiensis YBT-1518]
GTGGTAAAAGATTATGACAATGATTTTTTTAAAGAAGATAACGAAGATACAACAGATTTCTCTTTAATAAAAGGGGATAC
GTTGCAGCAAGTAGAAGAATTAGAAGAAGAATTAAAAAGAAAATCATAA

Structures

Antitoxin

• AlphaFold DB: W8YZL5