Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemIK/MazF(toxin)
Location 244426..245068 Replicon chromosome
Accession NC_022873
Organism Bacillus thuringiensis YBT-1518

Toxin (Protein)


Gene name pemK Uniprot ID R8I8B8
Locus tag YBT1518_RS01385 Protein ID WP_000635965.1
Coordinates 244718..245068 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID R8I8H2
Locus tag YBT1518_RS01380 Protein ID WP_000004570.1
Coordinates 244426..244713 (+) Length 96 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
YBT1518_RS01355 239743..240705 + 963 WP_000961144.1 UV DNA damage repair endonuclease UvsE -
YBT1518_RS01360 240698..241270 - 573 WP_000908526.1 rhomboid family intramembrane serine protease -
YBT1518_RS01365 241364..241723 + 360 WP_000583419.1 holo-ACP synthase -
YBT1518_RS01370 241880..242830 + 951 WP_002094231.1 outer membrane lipoprotein carrier protein LolA -
YBT1518_RS01375 242948..244117 + 1170 WP_000390593.1 alanine racemase -
YBT1518_RS01380 244426..244713 + 288 WP_000004570.1 antitoxin EndoAI Antitoxin
YBT1518_RS01385 244718..245068 + 351 WP_000635965.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
YBT1518_RS01390 245137..247305 + 2169 WP_000426207.1 RNA-binding transcriptional accessory protein -
YBT1518_RS34010 247363..247479 - 117 WP_001143640.1 cortex morphogenetic protein CmpA -
YBT1518_RS01395 247675..248133 + 459 WP_000503730.1 SprT family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12948.04 Da        Isoelectric Point: 5.7168

>T30327 WP_000635965.1 NC_022873:244718-245068 [Bacillus thuringiensis YBT-1518]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTVIVAAITAQIQKAKLPTHVEIDAKKYGFERDSVILLEQI
RTIDKQRLTDKITHLDEVMMSRVDEALQISLGLIDF

Download         Length: 351 bp

>T30327 NC_022873:244718-245068 [Bacillus thuringiensis YBT-1518]
TTGATTGTAAAACGCGGCGACGTGTATTTTGCAGACCTTTCCCCAGTTGTTGGTTCTGAGCAAGGAGGCGTTCGTCCGGT
TCTTGTCATTCAAAATGACATCGGAAATCGTTTTAGTCCAACGGTGATTGTAGCGGCTATTACTGCACAGATTCAAAAAG
CGAAATTACCCACTCATGTGGAAATTGATGCGAAAAAGTATGGTTTTGAGAGAGATTCTGTTATTTTACTTGAGCAGATT
CGAACAATCGATAAGCAACGCTTAACGGACAAAATCACTCATTTAGATGAAGTGATGATGAGTCGTGTAGATGAAGCGTT
ACAAATTAGTTTAGGACTAATAGATTTTTAA

Antitoxin


Download         Length: 96 a.a.        Molecular weight: 10780.36 Da        Isoelectric Point: 8.9605

>AT30327 WP_000004570.1 NC_022873:244426-244713 [Bacillus thuringiensis YBT-1518]
VSESSVTTEIVVRLPKQMVTELDGIGKQENKNRHELICQATQLLLRQHKTKKRYQHESMRRGYIEMGKINLGIASEAFLA
EYEAAHTVERLVSGG

Download         Length: 288 bp

>AT30327 NC_022873:244426-244713 [Bacillus thuringiensis YBT-1518]
GTGTCCGAATCAAGTGTAACTACTGAAATCGTGGTTCGGTTGCCAAAGCAAATGGTAACGGAATTGGACGGAATTGGAAA
ACAAGAGAATAAGAATCGCCATGAACTAATTTGCCAGGCAACACAATTGTTATTGCGTCAACATAAGACGAAGAAACGCT
ACCAACATGAATCAATGCGACGTGGGTATATTGAAATGGGAAAAATTAATCTTGGTATTGCATCTGAAGCTTTCTTAGCG
GAGTATGAAGCAGCTCATACAGTAGAACGCTTAGTTAGCGGGGGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A366G118

Antitoxin

Source ID Structure
AlphaFold DB A0A366FY90

References