Detailed information of TA system TA030321

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): parDE/RHH(antitoxin)
• Location: 152806..153311
• Replicon: chromosome
• Accession: NC_022808
• Organism: Pseudomonas aeruginosa PA1

Toxin (Protein)

• Gene name: parE
• Uniprot ID: -
• Locus tag: PA1S_RS00665
• Protein ID: WP_023518248.1
• Coordinates: 152806..153087 (-)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: parD
• Uniprot ID: A0A1C7BDS9
• Locus tag: PA1S_RS00670
• Protein ID: WP_003083775.1
• Coordinates: 153084..153311 (-)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
PA1S_RS00640 (PA1S_00635)	148057..149406	+	1350	WP_003142411.1	C4-dicarboxylate transporter DctA	-
PA1S_RS00645 (PA1S_00640)	149455..150141	+	687	WP_003083762.1	FadR family transcriptional regulator	-
PA1S_RS00650 (PA1S_00645)	150242..150976	+	735	WP_003117208.1	GntR family transcriptional regulator	-
PA1S_RS00655 (PA1S_00650)	151180..151566	+	387	WP_014602345.1	aegerolysin family protein	-
PA1S_RS00660 (PA1S_00655)	151598..152506	-	909	WP_003083769.1	LysR family transcriptional regulator	-
PA1S_RS00665 (PA1S_00660)	152806..153087	-	282	WP_023518248.1	type II toxin-antitoxin system toxin ParE	Toxin
PA1S_RS00670 (PA1S_00665)	153084..153311	-	228	WP_003083775.1	CopG family ribbon-helix-helix protein	Antitoxin
PA1S_RS00675 (PA1S_00670)	153487..154107	-	621	WP_003101226.1	hypothetical protein	-
PA1S_RS00680 (PA1S_00675)	154208..154708	+	501	WP_003101228.1	LEA type 2 family protein	-
PA1S_RS00685 (PA1S_00680)	154781..155122	+	342	WP_003101229.1	alkylphosphonate utilization protein	-
PA1S_RS00690 (PA1S_00685)	155204..156631	-	1428	WP_003083784.1	GABA permease	-
PA1S_RS00695 (PA1S_00690)	156800..158293	-	1494	WP_003083788.1	CoA-acylating methylmalonate-semialdehyde dehydrogenase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (3-87)

Antitoxin

• Ribbon-helix-helix protein, CopG (5-42)

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 10472.23 Da
• Isoelectric Point: 10.0435

>T30321 WP_023518248.1 NC_022808:c153087-152806 [Pseudomonas aeruginosa PA1]
MSLKWTRKAAADLDAIYDHYVVLIGPEKALKAVQDIVEQVKPLQQVANQGAGRPSEVPGVRTLPLERWPFSAPFRVKGKE
IQILRIDRIEITP

Nucleotide

• Length: 282 bp

>T30321 NC_022808:c153087-152806 [Pseudomonas aeruginosa PA1]
ATGAGCCTGAAGTGGACCCGCAAGGCGGCCGCCGACCTGGACGCCATCTACGACCATTACGTCGTGCTGATCGGCCCGGA
AAAAGCTCTGAAAGCCGTTCAGGACATTGTCGAGCAGGTGAAACCGCTGCAGCAGGTAGCCAACCAGGGGGCAGGGCGGC
CCAGCGAGGTGCCAGGCGTACGCACCCTGCCCCTGGAGCGCTGGCCGTTCAGCGCCCCGTTTCGGGTTAAAGGCAAGGAA
ATCCAGATTTTGCGCATCGACAGGATCGAAATTACCCCCTGA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8556.58 Da
• Isoelectric Point: 5.5740

>AT30321 WP_003083775.1 NC_022808:c153311-153084 [Pseudomonas aeruginosa PA1]
MSTVVSFRADDALVAALDELARATHRDRPYHLRQALAQYLERQQWQVAAIDEGLADANAGRLLEHTEIEKRWGLQ

Nucleotide

• Length: 228 bp

>AT30321 NC_022808:c153311-153084 [Pseudomonas aeruginosa PA1]
ATGAGCACCGTAGTCTCGTTCCGCGCCGATGACGCCCTGGTCGCGGCCCTCGACGAACTGGCCCGCGCCACCCACCGCGA
CCGCCCCTACCACCTGCGGCAGGCGCTCGCGCAGTACCTGGAAAGGCAGCAGTGGCAGGTCGCTGCCATCGATGAAGGCT
TGGCCGATGCCAATGCCGGTCGCCTGCTGGAACACACCGAGATCGAGAAGCGCTGGGGGCTGCAATGA

Structures

Antitoxin

• AlphaFold DB: A0A1C7BDS9