30314

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemIK/MazF(toxin)
Location 2854687..2855329 Replicon chromosome
Accession NC_022781
Organism Bacillus toyonensis BCT-7112

Toxin (Protein)


Gene name pemK Uniprot ID J8CWW5
Locus tag BTOYO_RS14790 Protein ID WP_000635963.1
Coordinates 2854979..2855329 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID R8I8H2
Locus tag BTOYO_RS14785 Protein ID WP_000004570.1
Coordinates 2854687..2854974 (+) Length 96 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BTOYO_RS14760 2850014..2850976 + 963 WP_000961174.1 UV DNA damage repair endonuclease UvsE -
BTOYO_RS14765 2850969..2851541 - 573 WP_000908503.1 rhomboid family intramembrane serine protease -
BTOYO_RS14770 2851634..2851993 + 360 WP_000635048.1 holo-ACP synthase -
BTOYO_RS14775 2852150..2853100 + 951 WP_002037834.1 outer membrane lipoprotein carrier protein LolA -
BTOYO_RS14780 2853218..2854387 + 1170 WP_000390621.1 alanine racemase -
BTOYO_RS14785 2854687..2854974 + 288 WP_000004570.1 antitoxin EndoAI Antitoxin
BTOYO_RS14790 2854979..2855329 + 351 WP_000635963.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
BTOYO_RS14795 2855398..2857566 + 2169 WP_000426215.1 RNA-binding transcriptional accessory protein -
BTOYO_RS26475 2857624..2857740 - 117 WP_001143642.1 cortex morphogenetic protein CmpA -
BTOYO_RS14800 2857948..2858415 + 468 WP_000544525.1 SprT family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(1-113)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12974.12 Da        Isoelectric Point: 5.7168

>T30314 WP_000635963.1 NC_022781:2854979-2855329 [Bacillus toyonensis BCT-7112]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTVIVAAITAQIQKAKLPTHVEIDAKKYGFERDSVILLEQI
RTIDKQRLTDKITHLDEVMMIRVDEALQISLGLIDF

Download         Length: 351 bp

>T30314 NC_022781:2854979-2855329 [Bacillus toyonensis BCT-7112]
TTGATTGTAAAACGCGGCGACGTGTATTTTGCAGACCTTTCCCCAGTTGTTGGTTCTGAGCAAGGAGGCGTTCGTCCGGT
TCTTGTCATTCAAAATGACATCGGAAATCGTTTTAGTCCAACGGTGATTGTAGCGGCTATTACTGCACAGATTCAAAAAG
CGAAATTACCCACTCATGTGGAAATTGATGCGAAAAAGTATGGATTTGAGAGAGATTCTGTTATTTTACTTGAGCAGATT
CGAACAATCGATAAGCAACGCTTAACGGACAAAATCACTCATTTGGATGAAGTGATGATGATTCGTGTAGATGAAGCGTT
ACAAATTAGTTTAGGACTAATAGATTTTTAA

Antitoxin


Download         Length: 96 a.a.        Molecular weight: 10780.36 Da        Isoelectric Point: 8.9605

>AT30314 WP_000004570.1 NC_022781:2854687-2854974 [Bacillus toyonensis BCT-7112]
VSESSVTTEIVVRLPKQMVTELDGIGKQENKNRHELICQATQLLLRQHKTKKRYQHESMRRGYIEMGKINLGIASEAFLA
EYEAAHTVERLVSGG

Download         Length: 288 bp

>AT30314 NC_022781:2854687-2854974 [Bacillus toyonensis BCT-7112]
GTGTCCGAATCAAGTGTAACTACTGAAATCGTGGTTCGGTTGCCAAAGCAAATGGTAACGGAATTGGACGGAATTGGAAA
ACAAGAGAATAAGAATCGCCATGAACTAATTTGCCAGGCAACACAACTGTTATTGCGTCAACATAAGACGAAGAAACGCT
ACCAACATGAATCAATGCGACGTGGGTACATTGAAATGGGAAAAATTAATCTTGGTATTGCATCTGAAGCTTTCTTAGCA
GAGTATGAAGCAGCTCATACGGTAGAACGCTTAGTTAGCGGGGGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4HKE
PDB 7BXY

Antitoxin

Source ID Structure
AlphaFold DB A0A366FY90

References